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Article: Using isoprostanes as biomarkers of oxidative stress: Some rarely considered issues

TitleUsing isoprostanes as biomarkers of oxidative stress: Some rarely considered issues
Authors
Issue Date2010
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/ars
Citation
Antioxidants And Redox Signaling, 2010, v. 13 n. 2, p. 145-156 How to Cite?
AbstractThe measurement of F2-isoprostanes by methods utilizing mass spectrometry is widely regarded as the best currently available biomarker of lipid peroxidation. F2-isoprostanes and their metabolites can be measured accurately in plasma, urine, and other body fluids using mass spectrometric techniques, and detailed protocols have been published in several papers. However, many clinical studies and intervention studies with diets or supplements, have employed single "spot" measurements of F 2-isoprostanes on either plasma/serum or urine to estimate "oxidative stress." This review examines the validity of the common assumption that plasma and urinary F2-isoprostane measurements are equivalent. It identifies scenarios where they may not be and where "spot" measurements can be misleading, with examples from the literature. We also discuss the controversial issue of whether and how F 2-isoprostane levels in plasma should be standardized against lipids, and, if so, which lipids to use. © Copyright 2010, Mary Ann Liebert, Inc. 2010.
Persistent Identifierhttp://hdl.handle.net/10722/179192
ISSN
2015 Impact Factor: 7.093
2015 SCImago Journal Rankings: 3.134
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHalliwell, Ben_US
dc.contributor.authorLee, CYJen_US
dc.date.accessioned2012-12-19T09:52:44Z-
dc.date.available2012-12-19T09:52:44Z-
dc.date.issued2010en_US
dc.identifier.citationAntioxidants And Redox Signaling, 2010, v. 13 n. 2, p. 145-156en_US
dc.identifier.issn1523-0864en_US
dc.identifier.urihttp://hdl.handle.net/10722/179192-
dc.description.abstractThe measurement of F2-isoprostanes by methods utilizing mass spectrometry is widely regarded as the best currently available biomarker of lipid peroxidation. F2-isoprostanes and their metabolites can be measured accurately in plasma, urine, and other body fluids using mass spectrometric techniques, and detailed protocols have been published in several papers. However, many clinical studies and intervention studies with diets or supplements, have employed single "spot" measurements of F 2-isoprostanes on either plasma/serum or urine to estimate "oxidative stress." This review examines the validity of the common assumption that plasma and urinary F2-isoprostane measurements are equivalent. It identifies scenarios where they may not be and where "spot" measurements can be misleading, with examples from the literature. We also discuss the controversial issue of whether and how F 2-isoprostane levels in plasma should be standardized against lipids, and, if so, which lipids to use. © Copyright 2010, Mary Ann Liebert, Inc. 2010.en_US
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/arsen_US
dc.relation.ispartofAntioxidants and Redox Signalingen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Markers - Chemistry - Metabolismen_US
dc.subject.meshBody Fluids - Chemistryen_US
dc.subject.meshF2-Isoprostanes - Chemistry - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshLipid Peroxidationen_US
dc.subject.meshLipids - Blooden_US
dc.subject.meshMass Spectrometry - Methodsen_US
dc.subject.meshMolecular Structureen_US
dc.subject.meshOxidative Stressen_US
dc.titleUsing isoprostanes as biomarkers of oxidative stress: Some rarely considered issuesen_US
dc.typeArticleen_US
dc.identifier.emailLee, CYJ: jettylee@hku.hken_US
dc.identifier.authorityLee, CYJ=rp01511en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1089/ars.2009.2934en_US
dc.identifier.pmid20001743-
dc.identifier.scopuseid_2-s2.0-77953313356en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953313356&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue2en_US
dc.identifier.spage145en_US
dc.identifier.epage156en_US
dc.identifier.isiWOS:000278527600003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHalliwell, B=7101878919en_US
dc.identifier.scopusauthoridLee, CYJ=13104265200en_US

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