File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Advanced fluorescence in situ hybridization to localize and quantify gene expression in japanese medaka (oryzias latipes) exposed to endocrine-disrupting compounds

TitleAdvanced fluorescence in situ hybridization to localize and quantify gene expression in japanese medaka (oryzias latipes) exposed to endocrine-disrupting compounds
Authors
Issue Date2009
PublisherSociety of Environmental Toxicology and Chemistry. The Journal's web site is located at http://etc.allenpress.com/
Citation
Environmental Toxicology And Chemistry, 2009, v. 28 n. 9, p. 1951-1962 How to Cite?
AbstractIn an earlier study, we described the development of fluorescence in situ hybridization (FISH) using confocal microscopy to localize and quantify gene expression in fish. Here, we report the results of FISH application to investigate effects of model endocrinedisrupting chemicals (EDCs), 17α-ethinylestradiol (EE2) and 17β-trenbolone (TB), on expressions of EDC-responsive genes in Japanese medaka (Oryzias latipes) at the cellular/tissue level paired, with histological observation. Gene expressions of vitellogenin-II (Vit-II), androgen receptor (AR), and cytochrome P450 gonadal aromatase (CYP19a) were determined after exposure to 5, 50, or 500 ng/L of EE2 or 50, 500, or 5,000 ng/L of TB for 7 d. Exposure to the greatest concentration of EE2 or TB significantly reduced fecundity and caused histological alterations in gonads. 17α-Ethinylestradiol induced. Vit-II expression in both male gonads and liver relative to controls and resulted in greater intensity of hematoxylin staining in hepatocytes, which was significantly correlated, with VitII induction in liver. When exposed to EE 2 at less than 50 ng/L, CYP19α expression associated with early stage oocytes was greater than that in controls. However, at 500 ng/L, this trend was reversed. The greater Vit-II expression in testis from all EE 2 groups, and the lesser expression of CYP19a in ovaries from the 500 ng/L group, likely is related to changes in the number of cells in which these genes are predominantly expressed rather than to an increase in expression per cell. 17β-Trenbolone significantly induced AR expression in ovaries but did not alter AR expression in female liver. It was concluded that FISH combined with histology enables advanced elucidation of molecular effects of chemicals by associating changes in gene expression with certain tissues and/or cell types and allows these changes to be related to histological effects. © 2009 SETAC.
Persistent Identifierhttp://hdl.handle.net/10722/179186
ISSN
2015 Impact Factor: 2.763
2015 SCImago Journal Rankings: 1.523
References

 

DC FieldValueLanguage
dc.contributor.authorPark, JWen_US
dc.contributor.authorTompsett, ARen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorNewsted, JLen_US
dc.contributor.authorJones, PDen_US
dc.contributor.authorDoris, WTAuen_US
dc.contributor.authorKong, Ren_US
dc.contributor.authorRudolf, SSWuen_US
dc.contributor.authorGiesy, JPen_US
dc.contributor.authorHecker, Men_US
dc.date.accessioned2012-12-19T09:52:39Z-
dc.date.available2012-12-19T09:52:39Z-
dc.date.issued2009en_US
dc.identifier.citationEnvironmental Toxicology And Chemistry, 2009, v. 28 n. 9, p. 1951-1962en_US
dc.identifier.issn0730-7268en_US
dc.identifier.urihttp://hdl.handle.net/10722/179186-
dc.description.abstractIn an earlier study, we described the development of fluorescence in situ hybridization (FISH) using confocal microscopy to localize and quantify gene expression in fish. Here, we report the results of FISH application to investigate effects of model endocrinedisrupting chemicals (EDCs), 17α-ethinylestradiol (EE2) and 17β-trenbolone (TB), on expressions of EDC-responsive genes in Japanese medaka (Oryzias latipes) at the cellular/tissue level paired, with histological observation. Gene expressions of vitellogenin-II (Vit-II), androgen receptor (AR), and cytochrome P450 gonadal aromatase (CYP19a) were determined after exposure to 5, 50, or 500 ng/L of EE2 or 50, 500, or 5,000 ng/L of TB for 7 d. Exposure to the greatest concentration of EE2 or TB significantly reduced fecundity and caused histological alterations in gonads. 17α-Ethinylestradiol induced. Vit-II expression in both male gonads and liver relative to controls and resulted in greater intensity of hematoxylin staining in hepatocytes, which was significantly correlated, with VitII induction in liver. When exposed to EE 2 at less than 50 ng/L, CYP19α expression associated with early stage oocytes was greater than that in controls. However, at 500 ng/L, this trend was reversed. The greater Vit-II expression in testis from all EE 2 groups, and the lesser expression of CYP19a in ovaries from the 500 ng/L group, likely is related to changes in the number of cells in which these genes are predominantly expressed rather than to an increase in expression per cell. 17β-Trenbolone significantly induced AR expression in ovaries but did not alter AR expression in female liver. It was concluded that FISH combined with histology enables advanced elucidation of molecular effects of chemicals by associating changes in gene expression with certain tissues and/or cell types and allows these changes to be related to histological effects. © 2009 SETAC.en_US
dc.languageengen_US
dc.publisherSociety of Environmental Toxicology and Chemistry. The Journal's web site is located at http://etc.allenpress.com/en_US
dc.relation.ispartofEnvironmental Toxicology and Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAromatase - Geneticsen_US
dc.subject.meshEndocrine Disruptors - Toxicityen_US
dc.subject.meshEthinyl Estradiol - Toxicityen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshIn Situ Hybridization, Fluorescence - Methodsen_US
dc.subject.meshMaleen_US
dc.subject.meshOryzias - Metabolismen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshReceptors, Androgen - Geneticsen_US
dc.subject.meshTrenbolone Acetate - Toxicityen_US
dc.subject.meshVitellogenins - Geneticsen_US
dc.titleAdvanced fluorescence in situ hybridization to localize and quantify gene expression in japanese medaka (oryzias latipes) exposed to endocrine-disrupting compoundsen_US
dc.typeArticleen_US
dc.identifier.emailRudolf, SSWu: rudolfwu@hku.hken_US
dc.identifier.authorityRudolf, SSWu=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1897/08-574.1en_US
dc.identifier.pmid19469586-
dc.identifier.scopuseid_2-s2.0-77950311389en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950311389&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume28en_US
dc.identifier.issue9en_US
dc.identifier.spage1951en_US
dc.identifier.epage1962en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPark, JW=8414402800en_US
dc.identifier.scopusauthoridTompsett, AR=12244509300en_US
dc.identifier.scopusauthoridZhang, X=36086912900en_US
dc.identifier.scopusauthoridNewsted, JL=6603677236en_US
dc.identifier.scopusauthoridJones, PD=34771015600en_US
dc.identifier.scopusauthoridDoris, WTAu=39862885500en_US
dc.identifier.scopusauthoridKong, R=7005290687en_US
dc.identifier.scopusauthoridRudolf, SSWu=7402945079en_US
dc.identifier.scopusauthoridGiesy, JP=35459135300en_US
dc.identifier.scopusauthoridHecker, M=35247848500en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats