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Article: Chapter 42 Techniques to Isolate O2-Sensitive Proteins. [4Fe-4S]-FNR as an Example

TitleChapter 42 Techniques to Isolate O2-Sensitive Proteins. [4Fe-4S]-FNR as an Example
Other TitlesTechniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879
Citation
Methods In Enzymology, 2009, v. 463 C, p. 787-805 How to Cite?
AbstractMany key enzymes in biological redox reactions require metal centers or cofactors for optimum activity and function. While the metal centers provide unique properties for protein structure and function, some also render protein activity sensitive to environmental O2 and cause experimental challenges to isolation and biochemical analysis. Iron-sulfur (Fe-S) clusters represent an important class of such metal centers and Fe-S proteins are widely distributed in nature. Here, we utilize FNR, a regulatory Fe-S protein from Escherichia coli, as an example to describe the techniques essential to purifying O2-labile proteins and summarize various approaches for their biochemical analysis. These methods can be readily adapted to purify other O2-labile proteins and advance our understanding of this interesting class of proteins. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179170
ISSN
2014 Impact Factor: 2.088
2015 SCImago Journal Rankings: 1.501
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYan, Aen_US
dc.contributor.authorKiley, PJen_US
dc.date.accessioned2012-12-19T09:52:31Z-
dc.date.available2012-12-19T09:52:31Z-
dc.date.issued2009en_US
dc.identifier.citationMethods In Enzymology, 2009, v. 463 C, p. 787-805en_US
dc.identifier.issn0076-6879en_US
dc.identifier.urihttp://hdl.handle.net/10722/179170-
dc.description.abstractMany key enzymes in biological redox reactions require metal centers or cofactors for optimum activity and function. While the metal centers provide unique properties for protein structure and function, some also render protein activity sensitive to environmental O2 and cause experimental challenges to isolation and biochemical analysis. Iron-sulfur (Fe-S) clusters represent an important class of such metal centers and Fe-S proteins are widely distributed in nature. Here, we utilize FNR, a regulatory Fe-S protein from Escherichia coli, as an example to describe the techniques essential to purifying O2-labile proteins and summarize various approaches for their biochemical analysis. These methods can be readily adapted to purify other O2-labile proteins and advance our understanding of this interesting class of proteins. © 2009 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.sciencedirect.com/science/bookseries/00766879en_US
dc.relation.ispartofMethods in Enzymologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacteriological Techniques - Methodsen_US
dc.subject.meshCell Fractionation - Methodsen_US
dc.subject.meshDrug Resistance - Physiologyen_US
dc.subject.meshEscherichia Coli Proteins - Analysis - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshIron-Sulfur Proteins - Analysis - Isolation & Purificationen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshOxygen - Pharmacologyen_US
dc.subject.meshProteins - Chemistry - Drug Effects - Isolation & Purificationen_US
dc.titleChapter 42 Techniques to Isolate O2-Sensitive Proteins. [4Fe-4S]-FNR as an Exampleen_US
dc.title.alternativeTechniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example-
dc.typeArticleen_US
dc.identifier.emailYan, A: ayan8@hku.hken_US
dc.identifier.authorityYan, A=rp00823en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0076-6879(09)63042-1en_US
dc.identifier.pmid19892202-
dc.identifier.scopuseid_2-s2.0-71549142859en_US
dc.identifier.hkuros220835-
dc.identifier.hkuros159702-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71549142859&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume463en_US
dc.identifier.issueCen_US
dc.identifier.spage787en_US
dc.identifier.epage805en_US
dc.identifier.isiWOS:000272201000042-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYan, A=8621667000en_US
dc.identifier.scopusauthoridKiley, PJ=7003423944en_US

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