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Article: Expression of proteinase inhibitor II proteins during floral development in Solanum americanum

TitleExpression of proteinase inhibitor II proteins during floral development in Solanum americanum
Authors
Issue Date2004
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2004, v. 219 n. 6, p. 1010-1022 How to Cite?
AbstractThe heterologous expression of serine proteinase inhibitor II (PIN2) proteins confers insect resistance in transgenic plants, but little is known of their endogenous roles. We have cloned two cDNAs encoding Solanum americanum PIN2 proteins, SaPIN2a and SaPIN2b. SaPIN2a is highly expressed in stem, particularly in the phloem, suggesting it could possibly regulate proteolysis in the sieve elements. When SaPIN2a was expressed in transgenic lettuce, we observed an inhibition of endogenous trypsin- and chymotrypsin-like activities. Here, we demonstrate that both SaPIN2a and SaPIN2b are expressed in floral tissues that are destined to undergo developmental programmed cell death (PCD), suggesting possible endogenous roles in inhibiting trypsin- and chymotrypsin-like activities during flower development. Northern and western blot analyses revealed that SaPIN2a and SaPIN2b mRNAs and proteins show highest expression early in floral development. In situ hybridization analysis and immunolocalization on floral sections, localized SaPIN2a and SaPIN2b mRNAs and their proteins to tissues that would apparently undergo PCD: the ovules, the stylar transmitting tissue, the stigma and the vascular bundles. Detection of PCD in floral sections was achieved using terminal deoxynucleotidyltransferase- mediated dUTP nick end labeling (TUNEL) analysis. Examination of the mid-style before, and 1 day after, pollination revealed that high expression of SaPIN2a and SaPIN2b in the style was inversely correlated with PCD. © Springer-Verlag 2004.
Persistent Identifierhttp://hdl.handle.net/10722/179164
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSin, SFen_US
dc.contributor.authorChye, MLen_US
dc.date.accessioned2012-12-19T09:52:28Z-
dc.date.available2012-12-19T09:52:28Z-
dc.date.issued2004en_US
dc.identifier.citationPlanta, 2004, v. 219 n. 6, p. 1010-1022en_US
dc.identifier.issn0032-0935en_US
dc.identifier.urihttp://hdl.handle.net/10722/179164-
dc.description.abstractThe heterologous expression of serine proteinase inhibitor II (PIN2) proteins confers insect resistance in transgenic plants, but little is known of their endogenous roles. We have cloned two cDNAs encoding Solanum americanum PIN2 proteins, SaPIN2a and SaPIN2b. SaPIN2a is highly expressed in stem, particularly in the phloem, suggesting it could possibly regulate proteolysis in the sieve elements. When SaPIN2a was expressed in transgenic lettuce, we observed an inhibition of endogenous trypsin- and chymotrypsin-like activities. Here, we demonstrate that both SaPIN2a and SaPIN2b are expressed in floral tissues that are destined to undergo developmental programmed cell death (PCD), suggesting possible endogenous roles in inhibiting trypsin- and chymotrypsin-like activities during flower development. Northern and western blot analyses revealed that SaPIN2a and SaPIN2b mRNAs and proteins show highest expression early in floral development. In situ hybridization analysis and immunolocalization on floral sections, localized SaPIN2a and SaPIN2b mRNAs and their proteins to tissues that would apparently undergo PCD: the ovules, the stylar transmitting tissue, the stigma and the vascular bundles. Detection of PCD in floral sections was achieved using terminal deoxynucleotidyltransferase- mediated dUTP nick end labeling (TUNEL) analysis. Examination of the mid-style before, and 1 day after, pollination revealed that high expression of SaPIN2a and SaPIN2b in the style was inversely correlated with PCD. © Springer-Verlag 2004.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_US
dc.relation.ispartofPlantaen_US
dc.subject.meshApoptosis - Physiologyen_US
dc.subject.meshFlowers - Growth & Development - Metabolismen_US
dc.subject.meshPlant Proteins - Biosynthesis - Physiologyen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRna, Plant - Metabolismen_US
dc.subject.meshSolanum - Growth & Development - Metabolismen_US
dc.subject.meshTissue Distributionen_US
dc.titleExpression of proteinase inhibitor II proteins during floral development in Solanum americanumen_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00425-004-1306-6en_US
dc.identifier.pmid15197596-
dc.identifier.scopuseid_2-s2.0-7044234943en_US
dc.identifier.hkuros96631-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-7044234943&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume219en_US
dc.identifier.issue6en_US
dc.identifier.spage1010en_US
dc.identifier.epage1022en_US
dc.identifier.isiWOS:000224614600010-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridSin, SF=7006553279en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US

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