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- Publisher Website: 10.1016/j.biombioe.2009.05.022
- Scopus: eid_2-s2.0-69449101792
- WOS: WOS:000270770000008
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Article: Rapid screening method for lipid production in alga based on Nile red fluorescence
Title | Rapid screening method for lipid production in alga based on Nile red fluorescence |
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Authors | |
Keywords | C. Vulgaris Experimental Design Fluorescence Dye Lipid, Chlorella Spp Lyophilization Nile Red Spectrofluorimetry, 9-Diethylamino-5H-Benzo[<Alpha>]Phenoxazine-5-One Strain Selection |
Issue Date | 2009 |
Publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/biombioe |
Citation | Biomass And Bioenergy, 2009, v. 33 n. 10, p. 1386-1392 How to Cite? |
Abstract | As a promising source of biodiesel, Chlorella has drawn more and more attention of researcher, while rapid screening of enhanced lipid production becomes essential. Here we investigated lyophilized alga powders as the starting material and optimized Nile red method for quantitative measurement of neutral lipids in Chlorella with a high correlation coefficient (R2 = 0.99) between gravimetric and spectrofluorimetric quantification. Using this method, Chlorella vulgaris were screened out of several Chlorella strains with the highest lipid content and orthogonal design experiments were performed to search for the significant factors affecting lipid accumulation. Our data implied that the sensitivity and versatility enable this method a useful tool in optimizing culture and accumulation condition of lipid production in alga and figured out several factors significantly influenced lipid production in C. vulgaris. © 2009 Elsevier Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/179155 |
ISSN | 2023 Impact Factor: 5.8 2023 SCImago Journal Rankings: 1.107 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Huang, GH | en_US |
dc.contributor.author | Chen, G | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:52:25Z | - |
dc.date.available | 2012-12-19T09:52:25Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Biomass And Bioenergy, 2009, v. 33 n. 10, p. 1386-1392 | en_US |
dc.identifier.issn | 0961-9534 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179155 | - |
dc.description.abstract | As a promising source of biodiesel, Chlorella has drawn more and more attention of researcher, while rapid screening of enhanced lipid production becomes essential. Here we investigated lyophilized alga powders as the starting material and optimized Nile red method for quantitative measurement of neutral lipids in Chlorella with a high correlation coefficient (R2 = 0.99) between gravimetric and spectrofluorimetric quantification. Using this method, Chlorella vulgaris were screened out of several Chlorella strains with the highest lipid content and orthogonal design experiments were performed to search for the significant factors affecting lipid accumulation. Our data implied that the sensitivity and versatility enable this method a useful tool in optimizing culture and accumulation condition of lipid production in alga and figured out several factors significantly influenced lipid production in C. vulgaris. © 2009 Elsevier Ltd. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/biombioe | en_US |
dc.relation.ispartof | Biomass and Bioenergy | en_US |
dc.subject | C. Vulgaris | en_US |
dc.subject | Experimental Design | en_US |
dc.subject | Fluorescence Dye | en_US |
dc.subject | Lipid, Chlorella Spp | en_US |
dc.subject | Lyophilization | en_US |
dc.subject | Nile Red | en_US |
dc.subject | Spectrofluorimetry, 9-Diethylamino-5H-Benzo[<Alpha>]Phenoxazine-5-One | en_US |
dc.subject | Strain Selection | en_US |
dc.title | Rapid screening method for lipid production in alga based on Nile red fluorescence | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.biombioe.2009.05.022 | en_US |
dc.identifier.scopus | eid_2-s2.0-69449101792 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-69449101792&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 33 | en_US |
dc.identifier.issue | 10 | en_US |
dc.identifier.spage | 1386 | en_US |
dc.identifier.epage | 1392 | en_US |
dc.identifier.isi | WOS:000270770000008 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Huang, GH=14040130100 | en_US |
dc.identifier.scopusauthorid | Chen, G=35309532100 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.citeulike | 5323636 | - |
dc.identifier.issnl | 0961-9534 | - |