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Article: Rapid screening method for lipid production in alga based on Nile red fluorescence

TitleRapid screening method for lipid production in alga based on Nile red fluorescence
Authors
KeywordsC. Vulgaris
Experimental Design
Fluorescence Dye
Lipid, Chlorella Spp
Lyophilization
Nile Red
Spectrofluorimetry, 9-Diethylamino-5H-Benzo[<Alpha>]Phenoxazine-5-One
Strain Selection
Issue Date2009
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/biombioe
Citation
Biomass And Bioenergy, 2009, v. 33 n. 10, p. 1386-1392 How to Cite?
AbstractAs a promising source of biodiesel, Chlorella has drawn more and more attention of researcher, while rapid screening of enhanced lipid production becomes essential. Here we investigated lyophilized alga powders as the starting material and optimized Nile red method for quantitative measurement of neutral lipids in Chlorella with a high correlation coefficient (R2 = 0.99) between gravimetric and spectrofluorimetric quantification. Using this method, Chlorella vulgaris were screened out of several Chlorella strains with the highest lipid content and orthogonal design experiments were performed to search for the significant factors affecting lipid accumulation. Our data implied that the sensitivity and versatility enable this method a useful tool in optimizing culture and accumulation condition of lipid production in alga and figured out several factors significantly influenced lipid production in C. vulgaris. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179155
ISSN
2015 Impact Factor: 3.249
2015 SCImago Journal Rankings: 1.580
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, GHen_US
dc.contributor.authorChen, Gen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:52:25Z-
dc.date.available2012-12-19T09:52:25Z-
dc.date.issued2009en_US
dc.identifier.citationBiomass And Bioenergy, 2009, v. 33 n. 10, p. 1386-1392en_US
dc.identifier.issn0961-9534en_US
dc.identifier.urihttp://hdl.handle.net/10722/179155-
dc.description.abstractAs a promising source of biodiesel, Chlorella has drawn more and more attention of researcher, while rapid screening of enhanced lipid production becomes essential. Here we investigated lyophilized alga powders as the starting material and optimized Nile red method for quantitative measurement of neutral lipids in Chlorella with a high correlation coefficient (R2 = 0.99) between gravimetric and spectrofluorimetric quantification. Using this method, Chlorella vulgaris were screened out of several Chlorella strains with the highest lipid content and orthogonal design experiments were performed to search for the significant factors affecting lipid accumulation. Our data implied that the sensitivity and versatility enable this method a useful tool in optimizing culture and accumulation condition of lipid production in alga and figured out several factors significantly influenced lipid production in C. vulgaris. © 2009 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/biombioeen_US
dc.relation.ispartofBiomass and Bioenergyen_US
dc.subjectC. Vulgarisen_US
dc.subjectExperimental Designen_US
dc.subjectFluorescence Dyeen_US
dc.subjectLipid, Chlorella Sppen_US
dc.subjectLyophilizationen_US
dc.subjectNile Reden_US
dc.subjectSpectrofluorimetry, 9-Diethylamino-5H-Benzo[<Alpha>]Phenoxazine-5-Oneen_US
dc.subjectStrain Selectionen_US
dc.titleRapid screening method for lipid production in alga based on Nile red fluorescenceen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.biombioe.2009.05.022en_US
dc.identifier.scopuseid_2-s2.0-69449101792en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69449101792&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume33en_US
dc.identifier.issue10en_US
dc.identifier.spage1386en_US
dc.identifier.epage1392en_US
dc.identifier.isiWOS:000270770000008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHuang, GH=14040130100en_US
dc.identifier.scopusauthoridChen, G=35309532100en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.citeulike5323636-

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