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Article: Fine structure characterization of amylopectins from grain amaranth starch

TitleFine structure characterization of amylopectins from grain amaranth starch
Authors
Issue Date2009
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/carres
Citation
Carbohydrate Research, 2009, v. 344 n. 13, p. 1701-1708 How to Cite?
AbstractThe aim of this study was to determine the fine structure of amylopectin from grain amaranth. Amaranthus amylopectin was hydrolyzed with α-amylase, and single clusters and a group of clusters (domain) were isolated by methanol precipitation. The domain and the clusters were treated with phosphorylase a and then β-amylase to remove all external chains, whereby the internal structure was obtained. The φ{symbol},β-limit dextrins were analyzed on Sepharose CL 6B. The average DP (degree of polymerization) and peak-DP values of fractions of clusters were 57 and 82, respectively; the values of the domain were 137 and 309, respectively. The unit chain length profiles were analyzed by high-performance anion-exchange chromatography with pulsed amperometric detector (HPAEC-PAD). The results showed that the domain fraction contained ∼2.2 clusters, and single clusters were composed of ∼13 chains. The φ{symbol},β-limit dextrins of the clusters were further hydrolyzed with α-amylase to characterize their building block composition. The average DP of the branched blocks was ∼11 and they contained on average ∼2.5 chains. Their average chain length, internal chain length, and degree of branching were approximately 4.3, 2.8, and 14, respectively. A cluster consisted of ∼6 branched blocks, and the internal chain length between the blocks was ∼6.8. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179153
ISSN
2015 Impact Factor: 1.817
2015 SCImago Journal Rankings: 0.612
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKong, Xen_US
dc.contributor.authorCorke, Hen_US
dc.contributor.authorBertoft, Een_US
dc.date.accessioned2012-12-19T09:52:24Z-
dc.date.available2012-12-19T09:52:24Z-
dc.date.issued2009en_US
dc.identifier.citationCarbohydrate Research, 2009, v. 344 n. 13, p. 1701-1708en_US
dc.identifier.issn0008-6215en_US
dc.identifier.urihttp://hdl.handle.net/10722/179153-
dc.description.abstractThe aim of this study was to determine the fine structure of amylopectin from grain amaranth. Amaranthus amylopectin was hydrolyzed with α-amylase, and single clusters and a group of clusters (domain) were isolated by methanol precipitation. The domain and the clusters were treated with phosphorylase a and then β-amylase to remove all external chains, whereby the internal structure was obtained. The φ{symbol},β-limit dextrins were analyzed on Sepharose CL 6B. The average DP (degree of polymerization) and peak-DP values of fractions of clusters were 57 and 82, respectively; the values of the domain were 137 and 309, respectively. The unit chain length profiles were analyzed by high-performance anion-exchange chromatography with pulsed amperometric detector (HPAEC-PAD). The results showed that the domain fraction contained ∼2.2 clusters, and single clusters were composed of ∼13 chains. The φ{symbol},β-limit dextrins of the clusters were further hydrolyzed with α-amylase to characterize their building block composition. The average DP of the branched blocks was ∼11 and they contained on average ∼2.5 chains. Their average chain length, internal chain length, and degree of branching were approximately 4.3, 2.8, and 14, respectively. A cluster consisted of ∼6 branched blocks, and the internal chain length between the blocks was ∼6.8. © 2009 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/carresen_US
dc.relation.ispartofCarbohydrate Researchen_US
dc.subject.meshAmaranthus - Chemistryen_US
dc.subject.meshAmylopectin - Chemistry - Metabolismen_US
dc.subject.meshDextrins - Chemistryen_US
dc.subject.meshKineticsen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshStarch - Chemistryen_US
dc.subject.meshAlpha-Amylases - Metabolismen_US
dc.titleFine structure characterization of amylopectins from grain amaranth starchen_US
dc.typeArticleen_US
dc.identifier.emailCorke, H: harold@hku.hken_US
dc.identifier.authorityCorke, H=rp00688en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.carres.2009.05.032en_US
dc.identifier.pmid19604502-
dc.identifier.scopuseid_2-s2.0-68949167939en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68949167939&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume344en_US
dc.identifier.issue13en_US
dc.identifier.spage1701en_US
dc.identifier.epage1708en_US
dc.identifier.isiWOS:000269991600016-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridKong, X=7202794616en_US
dc.identifier.scopusauthoridCorke, H=7007102942en_US
dc.identifier.scopusauthoridBertoft, E=6604042426en_US
dc.identifier.citeulike5203822-

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