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Article: Allantoin in human plasma, serum, and nasal-lining fluids as a biomarker of oxidative stress: Avoiding artifacts and establishing real in vivo concentrations

TitleAllantoin in human plasma, serum, and nasal-lining fluids as a biomarker of oxidative stress: Avoiding artifacts and establishing real in vivo concentrations
Authors
Issue Date2009
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/ars
Citation
Antioxidants And Redox Signaling, 2009, v. 11 n. 8, p. 1767-1776 How to Cite?
AbstractUrate is the terminal product of purine metabolism in primates, including humans. Urate is also an efficient scavenger of oxidizing species and is thought to be an important antioxidant in human body fluids. Allantoin, the major oxidation product of urate, has been suggested as a candidate biomarker of oxidative stress because it is not produced metabolically. Although urate is converted to allantoin under strongly alkaline pH, such conditions have been used in the past to facilitate extraction of allantoin. We evolved a method for the determination of allantoin concentrations in human plasma and serum by gas chromatography-mass spectrometry without such artifact. With this method, we show that alkaline conditions do indeed cause breakdown of urate, leading to significant overestimation of allantoin concentration in human samples. By using our alternative method, serum samples from 98 volunteers were analyzed, and allantoin levels were found to be significantly lower than was previously reported. The in vivo utility and sensitivity of our method was further evaluated in human nasal-lining fluids. We were able to demonstrate an ozone-induced increase in allantoin, in the absence of increases in either ascorbate or glutathione oxidation products. © Copyright 2009, Mary Ann Liebert, Inc. 2009.
Persistent Identifierhttp://hdl.handle.net/10722/179142
ISSN
2015 Impact Factor: 7.093
2015 SCImago Journal Rankings: 3.134
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGruber, Jen_US
dc.contributor.authorTang, SYen_US
dc.contributor.authorJenner, AMen_US
dc.contributor.authorMudway, Ien_US
dc.contributor.authorBlomberg, Aen_US
dc.contributor.authorBehndig, Aen_US
dc.contributor.authorKasiman, Ken_US
dc.contributor.authorLee, CYJen_US
dc.contributor.authorSeet, RCSen_US
dc.contributor.authorZhang, Wen_US
dc.contributor.authorChen, Cen_US
dc.contributor.authorKelly, FJen_US
dc.contributor.authorHalliwell, Ben_US
dc.date.accessioned2012-12-19T09:52:19Z-
dc.date.available2012-12-19T09:52:19Z-
dc.date.issued2009en_US
dc.identifier.citationAntioxidants And Redox Signaling, 2009, v. 11 n. 8, p. 1767-1776en_US
dc.identifier.issn1523-0864en_US
dc.identifier.urihttp://hdl.handle.net/10722/179142-
dc.description.abstractUrate is the terminal product of purine metabolism in primates, including humans. Urate is also an efficient scavenger of oxidizing species and is thought to be an important antioxidant in human body fluids. Allantoin, the major oxidation product of urate, has been suggested as a candidate biomarker of oxidative stress because it is not produced metabolically. Although urate is converted to allantoin under strongly alkaline pH, such conditions have been used in the past to facilitate extraction of allantoin. We evolved a method for the determination of allantoin concentrations in human plasma and serum by gas chromatography-mass spectrometry without such artifact. With this method, we show that alkaline conditions do indeed cause breakdown of urate, leading to significant overestimation of allantoin concentration in human samples. By using our alternative method, serum samples from 98 volunteers were analyzed, and allantoin levels were found to be significantly lower than was previously reported. The in vivo utility and sensitivity of our method was further evaluated in human nasal-lining fluids. We were able to demonstrate an ozone-induced increase in allantoin, in the absence of increases in either ascorbate or glutathione oxidation products. © Copyright 2009, Mary Ann Liebert, Inc. 2009.en_US
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/arsen_US
dc.relation.ispartofAntioxidants and Redox Signalingen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [Antioxidants and Redox Signaling] © [2009] [copyright Mary Ann Liebert, Inc.]; [Antioxidants and Redox Signaling] is available online at: http://www.liebertonline.com.-
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAllantoin - Blooden_US
dc.subject.meshArtifactsen_US
dc.subject.meshBiological Markers - Blooden_US
dc.subject.meshBody Fluids - Chemistryen_US
dc.subject.meshCross-Over Studiesen_US
dc.subject.meshDouble-Blind Methoden_US
dc.subject.meshFemaleen_US
dc.subject.meshGas Chromatography-Mass Spectrometryen_US
dc.subject.meshHumansen_US
dc.subject.meshIsoprostanes - Analysisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNasal Mucosa - Secretionen_US
dc.subject.meshOxidative Stressen_US
dc.titleAllantoin in human plasma, serum, and nasal-lining fluids as a biomarker of oxidative stress: Avoiding artifacts and establishing real in vivo concentrationsen_US
dc.typeArticleen_US
dc.identifier.emailLee, CYJ: jettylee@hku.hken_US
dc.identifier.authorityLee, CYJ=rp01511en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1089/ars.2008.2364en_US
dc.identifier.pmid19388825-
dc.identifier.scopuseid_2-s2.0-67650239461en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67650239461&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume11en_US
dc.identifier.issue8en_US
dc.identifier.spage1767en_US
dc.identifier.epage1776en_US
dc.identifier.isiWOS:000267488900001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGruber, J=16401558900en_US
dc.identifier.scopusauthoridTang, SY=7403436651en_US
dc.identifier.scopusauthoridJenner, AM=7006757910en_US
dc.identifier.scopusauthoridMudway, I=6602889316en_US
dc.identifier.scopusauthoridBlomberg, A=35242044200en_US
dc.identifier.scopusauthoridBehndig, A=12141276600en_US
dc.identifier.scopusauthoridKasiman, K=26644957500en_US
dc.identifier.scopusauthoridLee, CYJ=13104265200en_US
dc.identifier.scopusauthoridSeet, RCS=10045357300en_US
dc.identifier.scopusauthoridZhang, W=24451478200en_US
dc.identifier.scopusauthoridChen, C=14008265500en_US
dc.identifier.scopusauthoridKelly, FJ=7102252167en_US
dc.identifier.scopusauthoridHalliwell, B=7101878919en_US

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