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Article: Separation and purification of epimedin A, B, C, and icariin from the medicinal herb epimedium brevicornum maxim by dual-mode HSCCC

TitleSeparation and purification of epimedin A, B, C, and icariin from the medicinal herb epimedium brevicornum maxim by dual-mode HSCCC
Authors
Issue Date2009
PublisherPreston Publications, Inc. The Journal's web site is located at http://www.j-chrom-sci.com/
Citation
Journal Of Chromatographic Science, 2009, v. 47 n. 5, p. 337-340 How to Cite?
AbstractEpimedium brevicornum Maxim is a famous medicinal herb which has been widely used for the treatment of impotence, infertility, osteoporosis, cardiovascular diseases, amnesia, and senile functional diseases. A dual-mode high-speed counter-current chromatographic method is developed for the separation and purification of four bioactive flavonoids from the medicinal herb Epimedium brevicornum Maxim. The crude flavonoids are obtained by extraction with ethyl acetate and ethanol from the dried aerial parts of Epimedium brevicornum Maxim under sonication. Highspeed counter-current chromatography with a two-phase solvent system composed of n-butanol-ethyl acetate-water (3:7:10, v/v) is performed using a dual-mode method, which yields 3.2 mg of epimedin A at a purity of 98.2%, 5.5 mg of epimedin B at a purity of 92.6%, 12.7 mg of epimedin C at a purity of 90.4%, and 42.9 mg of icariin at a purity of 96.8% based on the HPLC analysis. The recoveries are 95.2%, 89.4%, 91.1%, and 94.8%, respectively. The isolation of these flavonoids in adequate amounts makes them readily available for structure-activity relationship studies and for quality control of the herbal medicine.
Persistent Identifierhttp://hdl.handle.net/10722/179135
ISSN
2015 Impact Factor: 1.32
2015 SCImago Journal Rankings: 0.448
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:52:15Z-
dc.date.available2012-12-19T09:52:15Z-
dc.date.issued2009en_US
dc.identifier.citationJournal Of Chromatographic Science, 2009, v. 47 n. 5, p. 337-340en_US
dc.identifier.issn0021-9665en_US
dc.identifier.urihttp://hdl.handle.net/10722/179135-
dc.description.abstractEpimedium brevicornum Maxim is a famous medicinal herb which has been widely used for the treatment of impotence, infertility, osteoporosis, cardiovascular diseases, amnesia, and senile functional diseases. A dual-mode high-speed counter-current chromatographic method is developed for the separation and purification of four bioactive flavonoids from the medicinal herb Epimedium brevicornum Maxim. The crude flavonoids are obtained by extraction with ethyl acetate and ethanol from the dried aerial parts of Epimedium brevicornum Maxim under sonication. Highspeed counter-current chromatography with a two-phase solvent system composed of n-butanol-ethyl acetate-water (3:7:10, v/v) is performed using a dual-mode method, which yields 3.2 mg of epimedin A at a purity of 98.2%, 5.5 mg of epimedin B at a purity of 92.6%, 12.7 mg of epimedin C at a purity of 90.4%, and 42.9 mg of icariin at a purity of 96.8% based on the HPLC analysis. The recoveries are 95.2%, 89.4%, 91.1%, and 94.8%, respectively. The isolation of these flavonoids in adequate amounts makes them readily available for structure-activity relationship studies and for quality control of the herbal medicine.en_US
dc.languageengen_US
dc.publisherPreston Publications, Inc. The Journal's web site is located at http://www.j-chrom-sci.com/en_US
dc.relation.ispartofJournal of Chromatographic Scienceen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshCountercurrent Distribution - Methodsen_US
dc.subject.meshEpimedium - Chemistryen_US
dc.subject.meshFlavonoids - Isolation & Purificationen_US
dc.titleSeparation and purification of epimedin A, B, C, and icariin from the medicinal herb epimedium brevicornum maxim by dual-mode HSCCCen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/chromsci/47.5.337-
dc.identifier.pmid19476698-
dc.identifier.scopuseid_2-s2.0-66749178871en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66749178871&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume47en_US
dc.identifier.issue5en_US
dc.identifier.spage337en_US
dc.identifier.epage340en_US
dc.identifier.isiWOS:000273676800004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, HB=25649944400en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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