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Article: High-light and sodium chloride stress differentially regulate the biosynthesis of astaxanthin in chlorella zofingiensis (chlorophyceae)

TitleHigh-light and sodium chloride stress differentially regulate the biosynthesis of astaxanthin in chlorella zofingiensis (chlorophyceae)
Authors
KeywordsAstaxanthin
Carotenoid Hydroxylase
Carotenoid Ketolase
Chlorella Zofingiensis
High-Light Stress
Nacl Stress
Reactive Oxygen Species
Issue Date2009
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPY
Citation
Journal Of Phycology, 2009, v. 45 n. 3, p. 635-641 How to Cite?
AbstractAlthough light and salt stresses are known to enhance the biosynthesis of astaxanthin in certain green algae, the mechanism of their action remains largely unknown. Here we report the regulation of the genes encoding phytoene desaturase (PDS), carotenoid hydroxylase (CHYb), and carotenoid ketolase (BKT) in the green alga Chlorella zofingiensis Dönz under light or sodium chloride (NaCl) stress. High-light illumination (150 μ;mol photons · m-2 · s-1) up-regulated the transcripts of PDS, CHYb, and BKT and greatly enhanced the biosynthesis of zeaxanthin, canthaxanthin, and astaxanthin. In contrast, sodium chloride stress only up-regulated the transcript of BKT and enhanced the biosynthesis of canthaxanthin and astaxanthin. High-light and NaCl stresses were revealed to result in an increase in the intracellular reactive oxygen species (ROS) level. By applying chemically generated ROS to the algal cells, we determined that 1O2 specifically up-regulated the CHYb gene, whereas •OH up-regulated the PDS and BKT genes. We suggest that high-light and NaCl stresses may stimulate the generation of different ROS, which in turn trigger the up-regulation of specific carotenogenic genes and the enhanced biosynthesis of carotenoids in C. zofingiensis. © 2009 Phycological Society of America.
Persistent Identifierhttp://hdl.handle.net/10722/179134
ISSN
2015 Impact Factor: 2.536
2015 SCImago Journal Rankings: 1.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Yen_US
dc.contributor.authorHuang, Jen_US
dc.contributor.authorSandmann, Gen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:52:15Z-
dc.date.available2012-12-19T09:52:15Z-
dc.date.issued2009en_US
dc.identifier.citationJournal Of Phycology, 2009, v. 45 n. 3, p. 635-641en_US
dc.identifier.issn0022-3646en_US
dc.identifier.urihttp://hdl.handle.net/10722/179134-
dc.description.abstractAlthough light and salt stresses are known to enhance the biosynthesis of astaxanthin in certain green algae, the mechanism of their action remains largely unknown. Here we report the regulation of the genes encoding phytoene desaturase (PDS), carotenoid hydroxylase (CHYb), and carotenoid ketolase (BKT) in the green alga Chlorella zofingiensis Dönz under light or sodium chloride (NaCl) stress. High-light illumination (150 μ;mol photons · m-2 · s-1) up-regulated the transcripts of PDS, CHYb, and BKT and greatly enhanced the biosynthesis of zeaxanthin, canthaxanthin, and astaxanthin. In contrast, sodium chloride stress only up-regulated the transcript of BKT and enhanced the biosynthesis of canthaxanthin and astaxanthin. High-light and NaCl stresses were revealed to result in an increase in the intracellular reactive oxygen species (ROS) level. By applying chemically generated ROS to the algal cells, we determined that 1O2 specifically up-regulated the CHYb gene, whereas •OH up-regulated the PDS and BKT genes. We suggest that high-light and NaCl stresses may stimulate the generation of different ROS, which in turn trigger the up-regulation of specific carotenogenic genes and the enhanced biosynthesis of carotenoids in C. zofingiensis. © 2009 Phycological Society of America.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPYen_US
dc.relation.ispartofJournal of Phycologyen_US
dc.subjectAstaxanthinen_US
dc.subjectCarotenoid Hydroxylaseen_US
dc.subjectCarotenoid Ketolaseen_US
dc.subjectChlorella Zofingiensisen_US
dc.subjectHigh-Light Stressen_US
dc.subjectNacl Stressen_US
dc.subjectReactive Oxygen Speciesen_US
dc.titleHigh-light and sodium chloride stress differentially regulate the biosynthesis of astaxanthin in chlorella zofingiensis (chlorophyceae)en_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1529-8817.2009.00689.xen_US
dc.identifier.scopuseid_2-s2.0-66749155486en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66749155486&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue3en_US
dc.identifier.spage635en_US
dc.identifier.epage641en_US
dc.identifier.isiWOS:000266714200012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, Y=8875807300en_US
dc.identifier.scopusauthoridHuang, J=7407192967en_US
dc.identifier.scopusauthoridSandmann, G=7006654333en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.citeulike4834374-

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