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Article: In situ hybridization to detect spatial gene expression in medaka

TitleIn situ hybridization to detect spatial gene expression in medaka
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv
Citation
Ecotoxicology And Environmental Safety, 2009, v. 72 n. 4, p. 1257-1264 How to Cite?
AbstractA whole-animal tissue section in situ hybridization (ISH) system with radio-labeled probes was developed to detect differential gene expression among tissues of the small, oviparous teleost fish, Japanese medaka (Oryzias latipes). Because of its tissue- and gender-specific expression, gonadal aromatase (CYP19a) was selected as a model gene to demonstrate the potential of the system. The ISH system was validated with a 7 d exposure to the model aromatase inhibitor, fadrozole. Fadrozole did not affect the magnitude of gene expression in testes, but significantly up-regulated CYP19a gene expression in ovaries. These results were confirmed with quantitative real-time-polymerase chain reaction (RT-PCR). Histological evaluation revealed that females exposed to 100 μg/L fadrozole lacked mature oocytes. Male gonadal morphology was normal in all treatments. The ISH method developed in this study allowed tissue-specific resolution of gene expression in a whole animal model, as well as the ability to analyze cellular morphological detail in the same organism. © 2008 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179126
ISSN
2015 Impact Factor: 3.13
2015 SCImago Journal Rankings: 1.229
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTompsett, ARen_US
dc.contributor.authorPark, JWen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorJones, PDen_US
dc.contributor.authorNewsted, JLen_US
dc.contributor.authorAu, DWTen_US
dc.contributor.authorChen, EXHen_US
dc.contributor.authorYu, Ren_US
dc.contributor.authorWu, RSSen_US
dc.contributor.authorKong, RYCen_US
dc.contributor.authorGiesy, JPen_US
dc.contributor.authorHecker, Men_US
dc.date.accessioned2012-12-19T09:52:12Z-
dc.date.available2012-12-19T09:52:12Z-
dc.date.issued2009en_US
dc.identifier.citationEcotoxicology And Environmental Safety, 2009, v. 72 n. 4, p. 1257-1264en_US
dc.identifier.issn0147-6513en_US
dc.identifier.urihttp://hdl.handle.net/10722/179126-
dc.description.abstractA whole-animal tissue section in situ hybridization (ISH) system with radio-labeled probes was developed to detect differential gene expression among tissues of the small, oviparous teleost fish, Japanese medaka (Oryzias latipes). Because of its tissue- and gender-specific expression, gonadal aromatase (CYP19a) was selected as a model gene to demonstrate the potential of the system. The ISH system was validated with a 7 d exposure to the model aromatase inhibitor, fadrozole. Fadrozole did not affect the magnitude of gene expression in testes, but significantly up-regulated CYP19a gene expression in ovaries. These results were confirmed with quantitative real-time-polymerase chain reaction (RT-PCR). Histological evaluation revealed that females exposed to 100 μg/L fadrozole lacked mature oocytes. Male gonadal morphology was normal in all treatments. The ISH method developed in this study allowed tissue-specific resolution of gene expression in a whole animal model, as well as the ability to analyze cellular morphological detail in the same organism. © 2008 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenven_US
dc.relation.ispartofEcotoxicology and Environmental Safetyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBody Weight - Drug Effectsen_US
dc.subject.meshDna Primersen_US
dc.subject.meshDna, Complementary - Biosynthesis - Geneticsen_US
dc.subject.meshEstrogen Antagonists - Pharmacologyen_US
dc.subject.meshFadrozole - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFresh Water - Analysisen_US
dc.subject.meshGene Expression - Physiologyen_US
dc.subject.meshGrowth - Drug Effectsen_US
dc.subject.meshImage Processing, Computer-Assisteden_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMaleen_US
dc.subject.meshOryzias - Physiologyen_US
dc.subject.meshOvary - Metabolism - Pathologyen_US
dc.subject.meshRna Probes - Diagnostic Useen_US
dc.subject.meshRna, Messenger - Analysis - Biosynthesisen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTestis - Metabolism - Pathologyen_US
dc.titleIn situ hybridization to detect spatial gene expression in medakaen_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ecoenv.2008.10.013en_US
dc.identifier.pmid19147229-
dc.identifier.scopuseid_2-s2.0-64449084131en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-64449084131&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume72en_US
dc.identifier.issue4en_US
dc.identifier.spage1257en_US
dc.identifier.epage1264en_US
dc.identifier.isiWOS:000265767900034-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTompsett, AR=12244509300en_US
dc.identifier.scopusauthoridPark, JW=8414402800en_US
dc.identifier.scopusauthoridZhang, X=8606600100en_US
dc.identifier.scopusauthoridJones, PD=34771015600en_US
dc.identifier.scopusauthoridNewsted, JL=6603677236en_US
dc.identifier.scopusauthoridAu, DWT=7004909228en_US
dc.identifier.scopusauthoridChen, EXH=7402316091en_US
dc.identifier.scopusauthoridYu, R=9278574900en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US
dc.identifier.scopusauthoridKong, RYC=7005290687en_US
dc.identifier.scopusauthoridGiesy, JP=35459135300en_US
dc.identifier.scopusauthoridHecker, M=35247848500en_US

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