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Article: Simultaneous determination of three phytoecdysteroids in the roots of four medicinal plants from the genus Asparagus by HPLC

TitleSimultaneous determination of three phytoecdysteroids in the roots of four medicinal plants from the genus Asparagus by HPLC
Authors
Issue Date2009
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/5152
Citation
Phytochemical Analysis, 2009, v. 20 n. 1, p. 58-63 How to Cite?
AbstractIntroduction - The genus Asparagus is known to contain phytoecdysteroids that have been shown to exhibit many beneficial pharmacological properties such as improving lipid metabolism, modulating immunological responses, etc. Currently, knowledge about the contents of phytoecdysteroids in the roots of Asparagus species is limited and HPLC methods for their analyses are unsatisfactory. Objective - To develop an HPLC method for the simultaneous determination of three phytoecdysteroids, 20-hydroxyecdysone, ecdysone and ajugasterone C, in the roots of four Asparagus species. Methodology - Reference standards of phytoecdysteroids were isolated from the roots of Asparagus filicinus by open column chromatography. HPLC analysis was performed on an Alltima C18 column with gradient elution using aqueous 0.2% formic acid solution containing 0.2% isopropanol and acetonitrile. Results - All calibration curves showed good linear correlation coefficients (r2 > 0.9994) within the tested ranges. Limits of detection (S/N = 3) and quantification (S/N = 10) for the three analytes were less than 2.7 and 9.9 ng, respectively. Intra- and inter-day RSDs of retention times and peak areas were less than 2.61%. The recoveries were between 93.2 and 107.5%, and the RSDs were less than 3.83% for the root samples of A. filicinus. Conclusion - The HPLC method established is appropriate for the efficient quantitative and qualitative analyses of important phytoecdysteroids in Asparagus species. This study showed that A. filicinus is rich in phytoecdysteroids, especially 20-hydroxyecdysone. However the three studied phytoecdysteroids were not detected in A. cochinchinensis, A. officinalis and A. setaceus. Copyright © 2008 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/179117
ISSN
2015 Impact Factor: 2.497
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, JJen_US
dc.contributor.authorCheng, KWen_US
dc.contributor.authorWang, Hen_US
dc.contributor.authorYe, WCen_US
dc.contributor.authorLi, ETSen_US
dc.contributor.authorWang, Men_US
dc.date.accessioned2012-12-19T09:52:06Z-
dc.date.available2012-12-19T09:52:06Z-
dc.date.issued2009en_US
dc.identifier.citationPhytochemical Analysis, 2009, v. 20 n. 1, p. 58-63en_US
dc.identifier.issn0958-0344en_US
dc.identifier.urihttp://hdl.handle.net/10722/179117-
dc.description.abstractIntroduction - The genus Asparagus is known to contain phytoecdysteroids that have been shown to exhibit many beneficial pharmacological properties such as improving lipid metabolism, modulating immunological responses, etc. Currently, knowledge about the contents of phytoecdysteroids in the roots of Asparagus species is limited and HPLC methods for their analyses are unsatisfactory. Objective - To develop an HPLC method for the simultaneous determination of three phytoecdysteroids, 20-hydroxyecdysone, ecdysone and ajugasterone C, in the roots of four Asparagus species. Methodology - Reference standards of phytoecdysteroids were isolated from the roots of Asparagus filicinus by open column chromatography. HPLC analysis was performed on an Alltima C18 column with gradient elution using aqueous 0.2% formic acid solution containing 0.2% isopropanol and acetonitrile. Results - All calibration curves showed good linear correlation coefficients (r2 > 0.9994) within the tested ranges. Limits of detection (S/N = 3) and quantification (S/N = 10) for the three analytes were less than 2.7 and 9.9 ng, respectively. Intra- and inter-day RSDs of retention times and peak areas were less than 2.61%. The recoveries were between 93.2 and 107.5%, and the RSDs were less than 3.83% for the root samples of A. filicinus. Conclusion - The HPLC method established is appropriate for the efficient quantitative and qualitative analyses of important phytoecdysteroids in Asparagus species. This study showed that A. filicinus is rich in phytoecdysteroids, especially 20-hydroxyecdysone. However the three studied phytoecdysteroids were not detected in A. cochinchinensis, A. officinalis and A. setaceus. Copyright © 2008 John Wiley & Sons, Ltd.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/5152en_US
dc.relation.ispartofPhytochemical Analysisen_US
dc.rightsPhytochemical Analysis. Copyright © John Wiley & Sons Ltd.-
dc.subject.meshAsparagus Plant - Chemistryen_US
dc.subject.meshCalibrationen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshEcdysone - Chemistry - Isolation & Purificationen_US
dc.subject.meshEcdysteroids - Chemistryen_US
dc.subject.meshEcdysterone - Analogs & Derivatives - Chemistry - Isolation & Purificationen_US
dc.subject.meshPlant Roots - Chemistryen_US
dc.subject.meshPlants, Medicinal - Chemistryen_US
dc.subject.meshReference Standardsen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSolventsen_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshSpectrophotometry, Ultravioleten_US
dc.titleSimultaneous determination of three phytoecdysteroids in the roots of four medicinal plants from the genus Asparagus by HPLCen_US
dc.typeArticleen_US
dc.identifier.emailLi, ETS: etsli@hku.hken_US
dc.identifier.emailWang, M: mfwang@hku.hken_US
dc.identifier.authorityLi, ETS=rp00737en_US
dc.identifier.authorityWang, M=rp00800en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/pca.1097en_US
dc.identifier.pmid19086092-
dc.identifier.scopuseid_2-s2.0-60549098641en_US
dc.identifier.hkuros163913-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60549098641&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue1en_US
dc.identifier.spage58en_US
dc.identifier.epage63en_US
dc.identifier.isiWOS:000262213500008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWu, JJ=8298828200en_US
dc.identifier.scopusauthoridCheng, KW=12141247000en_US
dc.identifier.scopusauthoridWang, H=10240993900en_US
dc.identifier.scopusauthoridYe, WC=7201560816en_US
dc.identifier.scopusauthoridLi, ETS=14018169600en_US
dc.identifier.scopusauthoridWang, M=7406691844en_US

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