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Article: Comparative analysis of astaxanthin and its esters in the mutant E1 of Haematococcus pluvialis and other green algae by HPLC with a C30 column

TitleComparative analysis of astaxanthin and its esters in the mutant E1 of Haematococcus pluvialis and other green algae by HPLC with a C30 column
Authors
Issue Date2008
Publisher中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/
Citation
Science In China, Series C: Life Sciences, 2008, v. 51 n. 12, p. 1108-1115 How to Cite?
AbstractA gradient reversed-phase high-performance liquid chromatography (HPLC) method using a C30 column was developed for the simultaneous determination of astaxanthin, astaxanthin monoesters and astaxanthin diesters in the green algae Chlorococcum sp., Chlorella zofingiensis, Haematococcus pluvialis and the mutant E1, which was obtained from the mutagenesis of H. pluvialis by exposure to UV-irradiation and ethyl methanesulphonate (EMS) with subsequent screening using nicotine. The results showed that the contents of total astaxanthins including free astaxanthin and astaxanthin esters ranged from 1.4 to 30.9 mg/g dry biomass in these green algae. The lower total astaxanthin levels (< 2 mg/g dry biomass) were detected in the green algae Chlorococcum sp. and C. zofingiensis. The higher total astaxanthin levels (>16 mg/g dry biomass) were found in the green alga H. pluvialis and its mutant E1. It is notable that the mutant E1 is found to have considerably higher amounts of total astaxanthin (30.9 mg/g) as compared to the wild strain of H. pluvialis (16.1 mg/g). This indicates that UV-irradiation and EMS compound mutagenesis with subsequent screening using nicotine is an effective method for breeding of a high-producing astaxanthin strain of H. pluvialis. In addition, the green alga C. zofingiensis had a remarkably higher percentage of astaxanthin diesters (76.3% of total astaxanthins) and a remarkably lower percentage of astaxanthin monoesters (18.0% of total astaxanthins) in comparison with H. pluvialis (35.5% for diesters and 60.9% for monoesters), the mutant E1 (49.1% and 48.1%) and Chlorococcum sp. (18.0% and 58.6%). © 2008 Science in China Press and Springer-Verlag GmbH.
Persistent Identifierhttp://hdl.handle.net/10722/179108
ISSN
2011 Impact Factor: 1.61
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPeng, Jen_US
dc.contributor.authorXiang, Wen_US
dc.contributor.authorTang, Qen_US
dc.contributor.authorSun, Nen_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorYuan, Jen_US
dc.date.accessioned2012-12-19T09:52:02Z-
dc.date.available2012-12-19T09:52:02Z-
dc.date.issued2008en_US
dc.identifier.citationScience In China, Series C: Life Sciences, 2008, v. 51 n. 12, p. 1108-1115en_US
dc.identifier.issn1006-9305en_US
dc.identifier.urihttp://hdl.handle.net/10722/179108-
dc.description.abstractA gradient reversed-phase high-performance liquid chromatography (HPLC) method using a C30 column was developed for the simultaneous determination of astaxanthin, astaxanthin monoesters and astaxanthin diesters in the green algae Chlorococcum sp., Chlorella zofingiensis, Haematococcus pluvialis and the mutant E1, which was obtained from the mutagenesis of H. pluvialis by exposure to UV-irradiation and ethyl methanesulphonate (EMS) with subsequent screening using nicotine. The results showed that the contents of total astaxanthins including free astaxanthin and astaxanthin esters ranged from 1.4 to 30.9 mg/g dry biomass in these green algae. The lower total astaxanthin levels (< 2 mg/g dry biomass) were detected in the green algae Chlorococcum sp. and C. zofingiensis. The higher total astaxanthin levels (>16 mg/g dry biomass) were found in the green alga H. pluvialis and its mutant E1. It is notable that the mutant E1 is found to have considerably higher amounts of total astaxanthin (30.9 mg/g) as compared to the wild strain of H. pluvialis (16.1 mg/g). This indicates that UV-irradiation and EMS compound mutagenesis with subsequent screening using nicotine is an effective method for breeding of a high-producing astaxanthin strain of H. pluvialis. In addition, the green alga C. zofingiensis had a remarkably higher percentage of astaxanthin diesters (76.3% of total astaxanthins) and a remarkably lower percentage of astaxanthin monoesters (18.0% of total astaxanthins) in comparison with H. pluvialis (35.5% for diesters and 60.9% for monoesters), the mutant E1 (49.1% and 48.1%) and Chlorococcum sp. (18.0% and 58.6%). © 2008 Science in China Press and Springer-Verlag GmbH.en_US
dc.languageengen_US
dc.publisher中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/en_US
dc.relation.ispartofScience in China, Series C: Life Sciencesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChlorophyta - Chemistryen_US
dc.subject.meshChromatography, High Pressure Liquid - Instrumentation - Methodsen_US
dc.subject.meshEsters - Chemistryen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshXanthophylls - Chemistryen_US
dc.titleComparative analysis of astaxanthin and its esters in the mutant E1 of Haematococcus pluvialis and other green algae by HPLC with a C30 columnen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11427-008-0146-1en_US
dc.identifier.pmid19093085-
dc.identifier.scopuseid_2-s2.0-58149085934en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58149085934&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume51en_US
dc.identifier.issue12en_US
dc.identifier.spage1108en_US
dc.identifier.epage1115en_US
dc.identifier.isiWOS:000261832500008-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridPeng, J=37104829600en_US
dc.identifier.scopusauthoridXiang, W=24780158300en_US
dc.identifier.scopusauthoridTang, Q=25930619900en_US
dc.identifier.scopusauthoridSun, N=7202557210en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridYuan, J=7403401467en_US
dc.identifier.citeulike3819447-

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