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Article: Enhanced protection against oxidative stress in an astaxanthin- overproduction Haematococcus mutant (Chlorophyceae)

TitleEnhanced protection against oxidative stress in an astaxanthin- overproduction Haematococcus mutant (Chlorophyceae)
Authors
KeywordsAstaxanthin
Carotenogenesis
Flow Cytometry
Haematococcus Pluvialis
Oxidative Stress
Pigment Mutant
Issue Date2008
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09670262.asp
Citation
European Journal Of Phycology, 2008, v. 43 n. 4, p. 365-376 How to Cite?
AbstractMany unicellular green algae can become yellow or red in various natural habitats due to mass accumulation of a secondary carotenoid, such as lutein, or astaxanthin. The accumulation of secondary carotenoids is generally thought to be a survival strategy of the algae under photo-oxidative stress or other adverse environmental conditions. The physiological role of the carotenoids in stress response is less well understood at the subcellular or molecular level. In this study, a stable astaxanthin overproduction mutant (MT 2877) was isolated by chemical mutagenesis of a wild type (WT) of the green microalga Haematococcus pluvialis Flotow NIES-144. MT 2877 was identical to the WT with respect to morphology, pigment composition, and growth kinetics during the early vegetative stage of the life cycle. However, it had the ability to synthesize and accumulate about twice the astaxanthin content of the WT under high light, or under high light in the presence of excess amounts of ferrous sulphate and sodium acetate. Under stress, the mutant exhibited higher photosynthetic activities than the WT, based on considerably higher chlorophyll fluorescence induction, chlorophyll autofluorescence intensities, and oxygen evolution rates. Cell mortality caused by stress was reduced by half in the mutant culture compared with the WT. Enhanced protection of the mutant against stress is attributed to its accelerated carotenogenesis and accumulation of astaxanthin. Our results suggest that MT 2877, or other astaxanthin overproduction Haematococcus mutants, may offer dual benefits, as compared with the wild type, by increasing cellular astaxanthin content while reducing cell mortality during stress-induced carotenogenesis. © 2008 British Phycological Society.
Persistent Identifierhttp://hdl.handle.net/10722/179105
ISSN
2015 Impact Factor: 2.205
2015 SCImago Journal Rankings: 0.996
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, Zen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorSommerfeld, Men_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorHu, Qen_US
dc.date.accessioned2012-12-19T09:52:01Z-
dc.date.available2012-12-19T09:52:01Z-
dc.date.issued2008en_US
dc.identifier.citationEuropean Journal Of Phycology, 2008, v. 43 n. 4, p. 365-376en_US
dc.identifier.issn0967-0262en_US
dc.identifier.urihttp://hdl.handle.net/10722/179105-
dc.description.abstractMany unicellular green algae can become yellow or red in various natural habitats due to mass accumulation of a secondary carotenoid, such as lutein, or astaxanthin. The accumulation of secondary carotenoids is generally thought to be a survival strategy of the algae under photo-oxidative stress or other adverse environmental conditions. The physiological role of the carotenoids in stress response is less well understood at the subcellular or molecular level. In this study, a stable astaxanthin overproduction mutant (MT 2877) was isolated by chemical mutagenesis of a wild type (WT) of the green microalga Haematococcus pluvialis Flotow NIES-144. MT 2877 was identical to the WT with respect to morphology, pigment composition, and growth kinetics during the early vegetative stage of the life cycle. However, it had the ability to synthesize and accumulate about twice the astaxanthin content of the WT under high light, or under high light in the presence of excess amounts of ferrous sulphate and sodium acetate. Under stress, the mutant exhibited higher photosynthetic activities than the WT, based on considerably higher chlorophyll fluorescence induction, chlorophyll autofluorescence intensities, and oxygen evolution rates. Cell mortality caused by stress was reduced by half in the mutant culture compared with the WT. Enhanced protection of the mutant against stress is attributed to its accelerated carotenogenesis and accumulation of astaxanthin. Our results suggest that MT 2877, or other astaxanthin overproduction Haematococcus mutants, may offer dual benefits, as compared with the wild type, by increasing cellular astaxanthin content while reducing cell mortality during stress-induced carotenogenesis. © 2008 British Phycological Society.en_US
dc.languageengen_US
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09670262.aspen_US
dc.relation.ispartofEuropean Journal of Phycologyen_US
dc.subjectAstaxanthinen_US
dc.subjectCarotenogenesisen_US
dc.subjectFlow Cytometryen_US
dc.subjectHaematococcus Pluvialisen_US
dc.subjectOxidative Stressen_US
dc.subjectPigment Mutanten_US
dc.titleEnhanced protection against oxidative stress in an astaxanthin- overproduction Haematococcus mutant (Chlorophyceae)en_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1080/09670260802227736en_US
dc.identifier.scopuseid_2-s2.0-57349161891en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57349161891&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume43en_US
dc.identifier.issue4en_US
dc.identifier.spage365en_US
dc.identifier.epage376en_US
dc.identifier.isiWOS:000261194700004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHu, Z=11638983600en_US
dc.identifier.scopusauthoridLi, Y=8875807300en_US
dc.identifier.scopusauthoridSommerfeld, M=7007025132en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridHu, Q=26666082400en_US
dc.identifier.citeulike3765862-

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