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Article: Genetic characterization of Mortierella alpina by sequencing the 18S-28S ribosomal gene internal transcribed spacer region

TitleGenetic characterization of Mortierella alpina by sequencing the 18S-28S ribosomal gene internal transcribed spacer region
Authors
Issue Date2008
Citation
Letters In Applied Microbiology, 2008, v. 47 n. 4, p. 250-255 How to Cite?
AbstractAims: The aim of this study was to establish a genetic background of Mortierella alpina, which is a cosmopolitan, soil-inhabiting Zygomycete that also has important biotechnology potential. Methods and Results: A total of 44 18S-28S ribosomal gene internal transcribed spacer (ITS1-5.8S rDNA-ITS2 DNA) regions of M. alpina from three diverse locations (Far East Asia, North America and West-Central Europe) were sequenced and investigated. The sequences between M. alpina and the three closely related species (Mortierella macrocystis, Mortierella gamsii and Mortierella humilis) showed 74-84% sequence identity. When a phylogenetic tree was constructed with a neighbour-joining algorithm, four clades of M. alpina isolates were clearly distinct with high bootstrap values. In addition, isolates from the West-Central Europe were found to have the highest gene and nucleotide diversities. Conclusions: The ITS region was a suitable tool for distinguishing M. alpina from other closely related species. The region also provided information of the diversity of M. alpina. Significance and Impact of the Study: The study established a genetic background of M. alpina for identification and the diversity of M. alpina provided information for further isolation and screening of the fungus. © 2008 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/179085
ISSN
2015 Impact Factor: 1.579
2015 SCImago Journal Rankings: 0.641
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, SYen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:51:51Z-
dc.date.available2012-12-19T09:51:51Z-
dc.date.issued2008en_US
dc.identifier.citationLetters In Applied Microbiology, 2008, v. 47 n. 4, p. 250-255en_US
dc.identifier.issn0266-8254en_US
dc.identifier.urihttp://hdl.handle.net/10722/179085-
dc.description.abstractAims: The aim of this study was to establish a genetic background of Mortierella alpina, which is a cosmopolitan, soil-inhabiting Zygomycete that also has important biotechnology potential. Methods and Results: A total of 44 18S-28S ribosomal gene internal transcribed spacer (ITS1-5.8S rDNA-ITS2 DNA) regions of M. alpina from three diverse locations (Far East Asia, North America and West-Central Europe) were sequenced and investigated. The sequences between M. alpina and the three closely related species (Mortierella macrocystis, Mortierella gamsii and Mortierella humilis) showed 74-84% sequence identity. When a phylogenetic tree was constructed with a neighbour-joining algorithm, four clades of M. alpina isolates were clearly distinct with high bootstrap values. In addition, isolates from the West-Central Europe were found to have the highest gene and nucleotide diversities. Conclusions: The ITS region was a suitable tool for distinguishing M. alpina from other closely related species. The region also provided information of the diversity of M. alpina. Significance and Impact of the Study: The study established a genetic background of M. alpina for identification and the diversity of M. alpina provided information for further isolation and screening of the fungus. © 2008 The Authors.en_US
dc.languageengen_US
dc.relation.ispartofLetters in Applied Microbiologyen_US
dc.subject.meshDna, Ribosomal Spacer - Geneticsen_US
dc.subject.meshGenetic Variationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMortierella - Geneticsen_US
dc.subject.meshPhylogenyen_US
dc.titleGenetic characterization of Mortierella alpina by sequencing the 18S-28S ribosomal gene internal transcribed spacer regionen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1472-765X.2008.02427.xen_US
dc.identifier.pmid19241518en_US
dc.identifier.scopuseid_2-s2.0-52449106756en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-52449106756&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume47en_US
dc.identifier.issue4en_US
dc.identifier.spage250en_US
dc.identifier.epage255en_US
dc.identifier.isiWOS:000259362300004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHo, SY=23980055900en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.citeulike3317341-

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