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Article: Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae)

TitleGlucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae)
Authors
Issue Date2008
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2008, v. 228 n. 5, p. 735-743 How to Cite?
AbstractThe biosynthesis of the ketocarotenoid astaxanthin is a subject of scientific and industrial interest. The unicellular green alga Chlorella zofingiensis Dönz is able to grow and accumulate astaxanthin in the dark with exogenous glucose as sole carbon and energy source. In this study, the transcription of β-carotenoid ketolase (BKT) and β-carotenoid hydroxylase (CHYb) genes were surveyed to reveal the regulation of astaxanthin biosynthesis in dark-grown Chlorella zofingiensis. Coupled with glucose analogs and the hexokinase inhibitor glucosamine, we found that phosphorylation of glucose (glucose sensing) was essential to the increased transcription of BKT and CHYb genes and the accumulation of astaxanthin in the dark-grown cells. However, phosphorylation of glucose per se only up-regulated the transcription of CHYb and stimulated the synthesis of zeaxanthin. Blockage of the mitochondrial alternative pathway eliminated the glucose effects on the increased transcription of BKT and astaxanthin accumulation, suggesting that signals from alternative pathway was involved in the up-regulation of BKT transcription. In addition, citrate was shown to up-regulate the transcription of BKT independent of reactive oxygen species formation. Taken altogether, we conclude that in dark-grown Chlorella zofingiensis, the transcription of BKT and CHYb genes are differently regulated by the metabolism of glucose, through which the biosynthesis of astaxanthin is regulated. © 2008 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/179082
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Yen_US
dc.contributor.authorHuang, Jen_US
dc.contributor.authorSandmann, Gen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:51:50Z-
dc.date.available2012-12-19T09:51:50Z-
dc.date.issued2008en_US
dc.identifier.citationPlanta, 2008, v. 228 n. 5, p. 735-743en_US
dc.identifier.issn0032-0935en_US
dc.identifier.urihttp://hdl.handle.net/10722/179082-
dc.description.abstractThe biosynthesis of the ketocarotenoid astaxanthin is a subject of scientific and industrial interest. The unicellular green alga Chlorella zofingiensis Dönz is able to grow and accumulate astaxanthin in the dark with exogenous glucose as sole carbon and energy source. In this study, the transcription of β-carotenoid ketolase (BKT) and β-carotenoid hydroxylase (CHYb) genes were surveyed to reveal the regulation of astaxanthin biosynthesis in dark-grown Chlorella zofingiensis. Coupled with glucose analogs and the hexokinase inhibitor glucosamine, we found that phosphorylation of glucose (glucose sensing) was essential to the increased transcription of BKT and CHYb genes and the accumulation of astaxanthin in the dark-grown cells. However, phosphorylation of glucose per se only up-regulated the transcription of CHYb and stimulated the synthesis of zeaxanthin. Blockage of the mitochondrial alternative pathway eliminated the glucose effects on the increased transcription of BKT and astaxanthin accumulation, suggesting that signals from alternative pathway was involved in the up-regulation of BKT transcription. In addition, citrate was shown to up-regulate the transcription of BKT independent of reactive oxygen species formation. Taken altogether, we conclude that in dark-grown Chlorella zofingiensis, the transcription of BKT and CHYb genes are differently regulated by the metabolism of glucose, through which the biosynthesis of astaxanthin is regulated. © 2008 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_US
dc.relation.ispartofPlantaen_US
dc.subject.meshAlgal Proteins - Metabolismen_US
dc.subject.meshChlorella - Metabolismen_US
dc.subject.meshCitric Acid Cycle - Physiologyen_US
dc.subject.meshGlucose - Metabolism - Pharmacologyen_US
dc.subject.meshHexokinase - Metabolismen_US
dc.subject.meshMitochondria - Metabolismen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshOxygenases - Metabolismen_US
dc.subject.meshPhosphorylation - Drug Effectsen_US
dc.subject.meshReactive Oxygen Species - Metabolismen_US
dc.subject.meshXanthophylls - Metabolismen_US
dc.titleGlucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae)en_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00425-008-0775-4en_US
dc.identifier.pmid18597111-
dc.identifier.scopuseid_2-s2.0-51349146898en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51349146898&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume228en_US
dc.identifier.issue5en_US
dc.identifier.spage735en_US
dc.identifier.epage743en_US
dc.identifier.isiWOS:000259009000003-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLi, Y=8875807300en_US
dc.identifier.scopusauthoridHuang, J=7407192967en_US
dc.identifier.scopusauthoridSandmann, G=7006654333en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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