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Article: Responses of the medaka HPG axis PCR array and reproduction to prochloraz and ketoconazole

TitleResponses of the medaka HPG axis PCR array and reproduction to prochloraz and ketoconazole
Authors
Issue Date2008
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
Citation
Environmental Science And Technology, 2008, v. 42 n. 17, p. 6762-6769 How to Cite?
AbstractEffects of two model imidazole-type fungicides, prochloraz (PCZ) and ketoconazole (KTC), on the hypothalamic-pituitary-gonadal (HPG) axis of the Japanese medaka (Oryzias latipe) were examined by use of real time PCR (RT-PCR) array, Fourteen-week-old Japanese medaka were exposed for seven days to concentrations of PCZ or KTC from 3.0 to 300 μg/L Exposure to KTC or PCZ caused significant reduction of fecundity of Japanese medaka and down-regulated expression of estrogen receptor (ER)-α and egg precursors in livers of males and females. However, PCZ was more potent than KTC both in modulating transcription and causing lesser fecundity. Exposure to nominal 30 μg PCZ/L resulted in 50% less fecundity and significant down-regulation of vitellogenin // expression, but KTC did not cause such effects at this concentration. Exposure to PCZ caused a compensatory upregulation in cytochrome P450 c17αhydroxylase, 17,20-lyase (CYP17) and aromatase (CYP19) expression in the ovary, while KTC did not. Furthermore, the ecologically relevant end point, fecundity was log-log related to mRNA level of six genes in livers of females. © 2008 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/179073
ISSN
2015 Impact Factor: 5.393
2015 SCImago Journal Rankings: 2.664
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Xen_US
dc.contributor.authorHecker, Men_US
dc.contributor.authorJones, PDen_US
dc.contributor.authorNewsted, Jen_US
dc.contributor.authorAu, Den_US
dc.contributor.authorKong, Ren_US
dc.contributor.authorWu, RSSen_US
dc.contributor.authorGiesy, JPen_US
dc.date.accessioned2012-12-19T09:51:47Z-
dc.date.available2012-12-19T09:51:47Z-
dc.date.issued2008en_US
dc.identifier.citationEnvironmental Science And Technology, 2008, v. 42 n. 17, p. 6762-6769en_US
dc.identifier.issn0013-936Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179073-
dc.description.abstractEffects of two model imidazole-type fungicides, prochloraz (PCZ) and ketoconazole (KTC), on the hypothalamic-pituitary-gonadal (HPG) axis of the Japanese medaka (Oryzias latipe) were examined by use of real time PCR (RT-PCR) array, Fourteen-week-old Japanese medaka were exposed for seven days to concentrations of PCZ or KTC from 3.0 to 300 μg/L Exposure to KTC or PCZ caused significant reduction of fecundity of Japanese medaka and down-regulated expression of estrogen receptor (ER)-α and egg precursors in livers of males and females. However, PCZ was more potent than KTC both in modulating transcription and causing lesser fecundity. Exposure to nominal 30 μg PCZ/L resulted in 50% less fecundity and significant down-regulation of vitellogenin // expression, but KTC did not cause such effects at this concentration. Exposure to PCZ caused a compensatory upregulation in cytochrome P450 c17αhydroxylase, 17,20-lyase (CYP17) and aromatase (CYP19) expression in the ovary, while KTC did not. Furthermore, the ecologically relevant end point, fecundity was log-log related to mRNA level of six genes in livers of females. © 2008 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/esten_US
dc.relation.ispartofEnvironmental Science and Technologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshHypothalamo-Hypophyseal System - Drug Effectsen_US
dc.subject.meshImidazoles - Toxicityen_US
dc.subject.meshKetoconazole - Toxicityen_US
dc.subject.meshLiver - Drug Effects - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshOryziasen_US
dc.subject.meshOvary - Drug Effectsen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshReproduction - Drug Effectsen_US
dc.subject.meshTestis - Drug Effectsen_US
dc.subject.meshTranscription, Genetic - Drug Effectsen_US
dc.titleResponses of the medaka HPG axis PCR array and reproduction to prochloraz and ketoconazoleen_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/es800591ten_US
dc.identifier.pmid18800561-
dc.identifier.scopuseid_2-s2.0-50849091331en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-50849091331&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume42en_US
dc.identifier.issue17en_US
dc.identifier.spage6762en_US
dc.identifier.epage6769en_US
dc.identifier.isiWOS:000258883300073-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhang, X=8606600100en_US
dc.identifier.scopusauthoridHecker, M=35247848500en_US
dc.identifier.scopusauthoridJones, PD=34771015600en_US
dc.identifier.scopusauthoridNewsted, J=6603677236en_US
dc.identifier.scopusauthoridAu, D=7004909228en_US
dc.identifier.scopusauthoridKong, R=7005290687en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US
dc.identifier.scopusauthoridGiesy, JP=35459135300en_US

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