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Article: Measurement of F2-isoprostanes, hydroxyeicosatetraenoic products, and oxysterols from a single plasma sample

TitleMeasurement of F2-isoprostanes, hydroxyeicosatetraenoic products, and oxysterols from a single plasma sample
Authors
Issue Date2008
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed
Citation
Free Radical Biology And Medicine, 2008, v. 44 n. 7, p. 1314-1322 How to Cite?
AbstractOxidized lipids such as F2-isoprostanes (F2-IsoPs), hydroxyeicosatetraenoic acid products (HETEs), and cholesterol oxidation products (COPs) are widely believed to be involved in multiple diseases. Usually, each product is measured individually in separate blood samples. In this study we describe a method allowing us to measure F2-IsoPs, HETEs, COPs, and arachidonate using a single sample. Plasma (1 ml) samples from healthy volunteers were diluted with heavy isotopic standards, hydrolyzed in alkali with organic solvent, and then subjected to anionic-exchange solid-phase extraction (SPE). After the SPE column was washed, hexane and hexane/ethyl acetate portions were collected and combined for COPs measurement. Thereafter the column was loaded with hexane/ethanol/acetic acid and fractions were collected for total F2-IsoPs, total HETEs, and arachidonate measurement. All compounds in the eluates were measured by gas chromatography-mass spectrometry. The efficiency of SPE and reproducibility for all compounds measured were high. Levels of total F2-IsoPs (0.45 ± 0.26 ng/ml (n = 157)), total HETEs (34.06 ± 16.35 ng/ml (n = 21)), total arachidonate (68.36 ± 24.45 μg/ml (n = 33)), and COPs (7-ketocholesterol, 12.25 ± 6.56 ng / ml; 7β-hydroxycholesterol, 6.32 ± 3.46 ng / ml; 7α-hydroxycholesterol, 15.06 ± 7.06 ng/ml; 24-hydroxycholesterol, 41.39 ± 18.22 ng/ml; and 27-hydroxycholesterol, 29.08 ± 16.79 ng/ml (n = 26)) were recorded in healthy subjects (age range 20 to 66 years; average male to female ratio 1:1). © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179046
ISSN
2015 Impact Factor: 5.784
2015 SCImago Journal Rankings: 2.468
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, CYJen_US
dc.contributor.authorHuang, SHen_US
dc.contributor.authorJenner, AMen_US
dc.contributor.authorHalliwell, Ben_US
dc.date.accessioned2012-12-19T09:51:37Z-
dc.date.available2012-12-19T09:51:37Z-
dc.date.issued2008en_US
dc.identifier.citationFree Radical Biology And Medicine, 2008, v. 44 n. 7, p. 1314-1322en_US
dc.identifier.issn0891-5849en_US
dc.identifier.urihttp://hdl.handle.net/10722/179046-
dc.description.abstractOxidized lipids such as F2-isoprostanes (F2-IsoPs), hydroxyeicosatetraenoic acid products (HETEs), and cholesterol oxidation products (COPs) are widely believed to be involved in multiple diseases. Usually, each product is measured individually in separate blood samples. In this study we describe a method allowing us to measure F2-IsoPs, HETEs, COPs, and arachidonate using a single sample. Plasma (1 ml) samples from healthy volunteers were diluted with heavy isotopic standards, hydrolyzed in alkali with organic solvent, and then subjected to anionic-exchange solid-phase extraction (SPE). After the SPE column was washed, hexane and hexane/ethyl acetate portions were collected and combined for COPs measurement. Thereafter the column was loaded with hexane/ethanol/acetic acid and fractions were collected for total F2-IsoPs, total HETEs, and arachidonate measurement. All compounds in the eluates were measured by gas chromatography-mass spectrometry. The efficiency of SPE and reproducibility for all compounds measured were high. Levels of total F2-IsoPs (0.45 ± 0.26 ng/ml (n = 157)), total HETEs (34.06 ± 16.35 ng/ml (n = 21)), total arachidonate (68.36 ± 24.45 μg/ml (n = 33)), and COPs (7-ketocholesterol, 12.25 ± 6.56 ng / ml; 7β-hydroxycholesterol, 6.32 ± 3.46 ng / ml; 7α-hydroxycholesterol, 15.06 ± 7.06 ng/ml; 24-hydroxycholesterol, 41.39 ± 18.22 ng/ml; and 27-hydroxycholesterol, 29.08 ± 16.79 ng/ml (n = 26)) were recorded in healthy subjects (age range 20 to 66 years; average male to female ratio 1:1). © 2007 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomeden_US
dc.relation.ispartofFree Radical Biology and Medicineen_US
dc.subject.meshAcetates - Chemistryen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshArachidonic Acid - Chemistryen_US
dc.subject.meshF2-Isoprostanes - Analysis - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshGas Chromatography-Mass Spectrometry - Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshHydroxyeicosatetraenoic Acids - Analysis - Chemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSolvents - Chemistryen_US
dc.subject.meshSterols - Analysis - Metabolismen_US
dc.titleMeasurement of F2-isoprostanes, hydroxyeicosatetraenoic products, and oxysterols from a single plasma sampleen_US
dc.typeArticleen_US
dc.identifier.emailLee, CYJ: jettylee@hku.hken_US
dc.identifier.authorityLee, CYJ=rp01511en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.freeradbiomed.2007.12.026en_US
dc.identifier.pmid18226605-
dc.identifier.scopuseid_2-s2.0-40949116379en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40949116379&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume44en_US
dc.identifier.issue7en_US
dc.identifier.spage1314en_US
dc.identifier.epage1322en_US
dc.identifier.isiWOS:000254910600010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, CYJ=13104265200en_US
dc.identifier.scopusauthoridHuang, SH=8367750600en_US
dc.identifier.scopusauthoridJenner, AM=7006757910en_US
dc.identifier.scopusauthoridHalliwell, B=7101878919en_US

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