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Article: Development of a marine fish model for studying in vivo molecular responses in ecotoxicology

TitleDevelopment of a marine fish model for studying in vivo molecular responses in ecotoxicology
Authors
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2008, v. 86 n. 2, p. 131-141 How to Cite?
AbstractA protocol for fixation and processing of whole adult marine medaka (Oryzias melastigma) was developed in parallel with in situ hybridization (ISH) and immunohistochemistry (IHC) for molecular analysis of in vivo gene and protein responses in fish. Over 200 serial sagittal sections (5 μm) can be produced from a single adult medaka to facilitate simultaneous localization and quantification of gene-specific mRNAs and proteins in different tissues and subcellular compartments of a single fish. Stereological analysis (as measured by volume density, V v) was used to quantify ISH and IHC signals on tissue sections. Using the telomerase reverse transcriptase (omTERT) gene, omTERT and proliferating cell nuclear antigen (PCNA) proteins as examples, we demonstrated that it is possible to localize, quantify and correlate their tissue expression profiles in a whole fish system. Using chronic hypoxia (1.8 ± 0.2 mg O 2 L -1 for 3 months) as an environmental stressor, we were able to identify significant alterations in levels of omTERT mRNA, omTERT protein, PCNA (cell proliferation marker) and TUNEL (apoptosis) in livers of hypoxic O. melastigma (p < 0.05). Overall, the results suggest that O. melastigma can serve as a model marine fish for assessing multiple in vivo molecular responses to stresses in the marine environment. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179039
ISSN
2015 Impact Factor: 3.557
2015 SCImago Journal Rankings: 1.671
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKong, RYCen_US
dc.contributor.authorGiesy, JPen_US
dc.contributor.authorWu, RSSen_US
dc.contributor.authorChen, EXHen_US
dc.contributor.authorChiang, MWLen_US
dc.contributor.authorLim, PLen_US
dc.contributor.authorYuen, BBHen_US
dc.contributor.authorYip, BWPen_US
dc.contributor.authorMok, HOLen_US
dc.contributor.authorAu, DWTen_US
dc.date.accessioned2012-12-19T09:51:35Z-
dc.date.available2012-12-19T09:51:35Z-
dc.date.issued2008en_US
dc.identifier.citationAquatic Toxicology, 2008, v. 86 n. 2, p. 131-141en_US
dc.identifier.issn0166-445Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179039-
dc.description.abstractA protocol for fixation and processing of whole adult marine medaka (Oryzias melastigma) was developed in parallel with in situ hybridization (ISH) and immunohistochemistry (IHC) for molecular analysis of in vivo gene and protein responses in fish. Over 200 serial sagittal sections (5 μm) can be produced from a single adult medaka to facilitate simultaneous localization and quantification of gene-specific mRNAs and proteins in different tissues and subcellular compartments of a single fish. Stereological analysis (as measured by volume density, V v) was used to quantify ISH and IHC signals on tissue sections. Using the telomerase reverse transcriptase (omTERT) gene, omTERT and proliferating cell nuclear antigen (PCNA) proteins as examples, we demonstrated that it is possible to localize, quantify and correlate their tissue expression profiles in a whole fish system. Using chronic hypoxia (1.8 ± 0.2 mg O 2 L -1 for 3 months) as an environmental stressor, we were able to identify significant alterations in levels of omTERT mRNA, omTERT protein, PCNA (cell proliferation marker) and TUNEL (apoptosis) in livers of hypoxic O. melastigma (p < 0.05). Overall, the results suggest that O. melastigma can serve as a model marine fish for assessing multiple in vivo molecular responses to stresses in the marine environment. © 2007 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_US
dc.relation.ispartofAquatic Toxicologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnoxia - Pathology - Veterinaryen_US
dc.subject.meshAntibodies, Monoclonal - Analysis - Metabolismen_US
dc.subject.meshEcotoxicology - Methodsen_US
dc.subject.meshEnvironmental Monitoring - Methodsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profiling - Veterinaryen_US
dc.subject.meshGene Expression Regulation - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistry - Veterinaryen_US
dc.subject.meshIn Situ Hybridization - Veterinaryen_US
dc.subject.meshIn Situ Nick-End Labeling - Veterinaryen_US
dc.subject.meshMaleen_US
dc.subject.meshOryziasen_US
dc.subject.meshProliferating Cell Nuclear Antigen - Analysis - Biosynthesisen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshTelomerase - Analysis - Biosynthesisen_US
dc.subject.meshTissue Fixation - Methods - Veterinaryen_US
dc.titleDevelopment of a marine fish model for studying in vivo molecular responses in ecotoxicologyen_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.aquatox.2007.10.011en_US
dc.identifier.pmid18055030-
dc.identifier.scopuseid_2-s2.0-38849141075en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-38849141075&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume86en_US
dc.identifier.issue2en_US
dc.identifier.spage131en_US
dc.identifier.epage141en_US
dc.identifier.isiWOS:000253696200002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridKong, RYC=7005290687en_US
dc.identifier.scopusauthoridGiesy, JP=35459135300en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US
dc.identifier.scopusauthoridChen, EXH=7402316091en_US
dc.identifier.scopusauthoridChiang, MWL=36752877600en_US
dc.identifier.scopusauthoridLim, PL=7202592401en_US
dc.identifier.scopusauthoridYuen, BBH=7005598106en_US
dc.identifier.scopusauthoridYip, BWP=23487627000en_US
dc.identifier.scopusauthoridMok, HOL=7005842490en_US
dc.identifier.scopusauthoridAu, DWT=7004909228en_US

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