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Article: Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) as a Growth Hormone (GH)-releasing factor in grass carp: II. Solution structure of a brain-specific PACAP by nuclear magnetic resonance spectroscopy and functional studies on GH release and gene expression

TitlePituitary Adenylate Cyclase-Activating Polypeptide (PACAP) as a Growth Hormone (GH)-releasing factor in grass carp: II. Solution structure of a brain-specific PACAP by nuclear magnetic resonance spectroscopy and functional studies on GH release and gene expression
Authors
Issue Date2007
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2007, v. 148 n. 10, p. 5042-5059 How to Cite?
AbstractPituitary adenylate cyclase-activating polypeptide (PACAP) has been proposed to be the ancestral GHRH. Recently, using grass carp as a model for modern-day bony fish, we demonstrated that PACAP nerve fibers are present in close proximity to carp somatotrophs, and mammalian PACAPs can induce GH secretion in carp pituitary cells. To further examine the role of PACAP as a GH-releasing factor in fish, the structural identity of grass carp PACAP was established by molecular cloning. The newly cloned PACAP was found to be a single-copy gene and expressed in the brain but not other tissues. The mature peptides of PACAP, namely PACAP 27 and PACAP 38, were synthesized. As revealed by nuclear magnetic resonance spectroscopies, carp PACAP 38 is composed of a flexible N terminal from His 1 to Ile 5, an extended central helix from Phe 6 to Val 26, and a short helical tail in the C terminal from Arg 29 to Arg 34. The C-terminal helix is located after a hinge region at Leu 27 to Gly 28 and is absent in the solution structures of PACAP 27. The two forms of PACAPs were effective in elevating GH release and GH transcript expression in grass carp pituitary cells. These stimulatory effects occurred with parallel rises in cAMP and Ca 2+ entry via voltage-sensitive Ca 2+ channels in carp somatotrophs. The present study represents the first report for solution structures of nonmammalian PACAPs and provides evidence that a brain-specific isoform of PACAP in fish can stimulate GH synthesis and release at the pituitary level, presumably by activating the appropriate postreceptor signaling mechanisms. Copyright © 2007 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/179012
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKong, HSen_US
dc.contributor.authorZhou, Hen_US
dc.contributor.authorYang, Yen_US
dc.contributor.authorHe, Men_US
dc.contributor.authorJiang, Yen_US
dc.contributor.authorWong, AOLen_US
dc.date.accessioned2012-12-19T09:51:25Z-
dc.date.available2012-12-19T09:51:25Z-
dc.date.issued2007en_US
dc.identifier.citationEndocrinology, 2007, v. 148 n. 10, p. 5042-5059en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/179012-
dc.description.abstractPituitary adenylate cyclase-activating polypeptide (PACAP) has been proposed to be the ancestral GHRH. Recently, using grass carp as a model for modern-day bony fish, we demonstrated that PACAP nerve fibers are present in close proximity to carp somatotrophs, and mammalian PACAPs can induce GH secretion in carp pituitary cells. To further examine the role of PACAP as a GH-releasing factor in fish, the structural identity of grass carp PACAP was established by molecular cloning. The newly cloned PACAP was found to be a single-copy gene and expressed in the brain but not other tissues. The mature peptides of PACAP, namely PACAP 27 and PACAP 38, were synthesized. As revealed by nuclear magnetic resonance spectroscopies, carp PACAP 38 is composed of a flexible N terminal from His 1 to Ile 5, an extended central helix from Phe 6 to Val 26, and a short helical tail in the C terminal from Arg 29 to Arg 34. The C-terminal helix is located after a hinge region at Leu 27 to Gly 28 and is absent in the solution structures of PACAP 27. The two forms of PACAPs were effective in elevating GH release and GH transcript expression in grass carp pituitary cells. These stimulatory effects occurred with parallel rises in cAMP and Ca 2+ entry via voltage-sensitive Ca 2+ channels in carp somatotrophs. The present study represents the first report for solution structures of nonmammalian PACAPs and provides evidence that a brain-specific isoform of PACAP in fish can stimulate GH synthesis and release at the pituitary level, presumably by activating the appropriate postreceptor signaling mechanisms. Copyright © 2007 by The Endocrine Society.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.rightsEndocrinology . Copyright © The Endocrine Society.-
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBrain - Metabolismen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCarps - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCircular Dichroismen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshCyclic Amp - Biosynthesisen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGrowth Hormone - Genetics - Secretionen_US
dc.subject.meshGrowth Hormone-Releasing Hormone - Physiologyen_US
dc.subject.meshIntracellular Membranes - Metabolismen_US
dc.subject.meshMagnetic Resonance Spectroscopyen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOsmolar Concentrationen_US
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptide - Genetics - Pharmacology - Physiologyen_US
dc.subject.meshPituitary Gland - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshProtein Isoforms - Physiologyen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshTissue Distributionen_US
dc.titlePituitary Adenylate Cyclase-Activating Polypeptide (PACAP) as a Growth Hormone (GH)-releasing factor in grass carp: II. Solution structure of a brain-specific PACAP by nuclear magnetic resonance spectroscopy and functional studies on GH release and gene expressionen_US
dc.typeArticleen_US
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AOL=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/en.2007-0576en_US
dc.identifier.pmid17615143-
dc.identifier.scopuseid_2-s2.0-34748886625en_US
dc.identifier.hkuros142720-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34748886625&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume148en_US
dc.identifier.issue10en_US
dc.identifier.spage5042en_US
dc.identifier.epage5059en_US
dc.identifier.eissn1945-7170-
dc.identifier.isiWOS:000249525600059-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKong, HS=7201353072en_US
dc.identifier.scopusauthoridZhou, H=7404742310en_US
dc.identifier.scopusauthoridYang, Y=7409391816en_US
dc.identifier.scopusauthoridHe, M=7402609178en_US
dc.identifier.scopusauthoridJiang, Y=15061459000en_US
dc.identifier.scopusauthoridWong, AOL=7403147570en_US
dc.identifier.citeulike3813272-

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