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Article: Characterization of the 5-′-flanking transcriptional regulatory region of chicken growth hormone gene

TitleCharacterization of the 5-′-flanking transcriptional regulatory region of chicken growth hormone gene
Authors
KeywordsCgh 5′-Flanking Region
Luciferase Assay
Pit-1
Tre
Issue Date2004
PublisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/
Citation
Experimental Biology And Medicine, 2004, v. 229 n. 7, p. 640-649 How to Cite?
AbstractA 1727-bp fragment of 5′-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. Various lengths of the 5′-flanking region (122 to 1775 bp) was linked to a luciferase reporter gene, and its transcriptional regulation was examined by an in vitro transient transfection coupled with luciferase assay. Our results demonstrated that pituitary-specific transcription factor, Pit-1, is necessary and sufficient to confer a strong tissue-specific expression. Co-transfection with goldfish or chicken Pit-1 expression vectors significantly restored the luciferase expression in HeLa cells. Site-directed mutagenesis and mobility gel-shift assays further confirmed the position of the Pit-1 binding site at -113/-104. Moreover, a repressive thyroid hormone response element (TRE) was identified at -137/-74, and we propose that interactions between the TRE and Pit-1 sites may be required for its repressive effect.
Persistent Identifierhttp://hdl.handle.net/10722/179005
ISSN
2015 Impact Factor: 2.542
2015 SCImago Journal Rankings: 0.991
References

 

DC FieldValueLanguage
dc.contributor.authorIp, SCYen_US
dc.contributor.authorLau, JSen_US
dc.contributor.authorAu, WLen_US
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:51:22Z-
dc.date.available2012-12-19T09:51:22Z-
dc.date.issued2004en_US
dc.identifier.citationExperimental Biology And Medicine, 2004, v. 229 n. 7, p. 640-649en_US
dc.identifier.issn1535-3702en_US
dc.identifier.urihttp://hdl.handle.net/10722/179005-
dc.description.abstractA 1727-bp fragment of 5′-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. Various lengths of the 5′-flanking region (122 to 1775 bp) was linked to a luciferase reporter gene, and its transcriptional regulation was examined by an in vitro transient transfection coupled with luciferase assay. Our results demonstrated that pituitary-specific transcription factor, Pit-1, is necessary and sufficient to confer a strong tissue-specific expression. Co-transfection with goldfish or chicken Pit-1 expression vectors significantly restored the luciferase expression in HeLa cells. Site-directed mutagenesis and mobility gel-shift assays further confirmed the position of the Pit-1 binding site at -113/-104. Moreover, a repressive thyroid hormone response element (TRE) was identified at -137/-74, and we propose that interactions between the TRE and Pit-1 sites may be required for its repressive effect.en_US
dc.languageengen_US
dc.publisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/en_US
dc.relation.ispartofExperimental Biology and Medicineen_US
dc.subjectCgh 5′-Flanking Regionen_US
dc.subjectLuciferase Assayen_US
dc.subjectPit-1en_US
dc.subjectTreen_US
dc.titleCharacterization of the 5-′-flanking transcriptional regulatory region of chicken growth hormone geneen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-3442878975en_US
dc.identifier.hkuros101941-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3442878975&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume229en_US
dc.identifier.issue7en_US
dc.identifier.spage640en_US
dc.identifier.epage649en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridIp, SCY=36940461900en_US
dc.identifier.scopusauthoridLau, JS=35080638800en_US
dc.identifier.scopusauthoridAu, WL=36889001000en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US

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