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Article: Lactobacillus rhamnosus strain GG reduces aflatoxin B1 transport, metabolism, and toxicity in Caco-2 cells

TitleLactobacillus rhamnosus strain GG reduces aflatoxin B1 transport, metabolism, and toxicity in Caco-2 cells
Authors
Issue Date2007
Citation
Applied And Environmental Microbiology, 2007, v. 73 n. 12, p. 3958-3964 How to Cite?
AbstractThe probiotic Lactobacillus rhamnosus GG is able to bind the potent hepatocarcinogen aflatoxin B1 (AFB1) and thus potentially restrict its rapid absorption from the intestine. In this study we investigated the potential of GG to reduce AFB1 availability in vitro in Caco-2 cells adapted to express cytochrome P-450 (CYP) 3A4, such that both transport and toxicity could be assessed. Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. AFB1 levels in culture medium were measured by high-performance liquid chromatography. In CYP 3A4-induced monolayers, AFB1 transport from the apical to the basolateral chamber was reduced from 11.1% ± 1.9% to 6.4% ± 2.5% (P = 0.019) and to 3.3% ± 1.8% (P = 0.002) within the first hour in monolayers coincubated with GG (1 × 1010 and 5 × 1010 CFU/ml, respectively). GG (1 × 1010 and 5 × 1010 CFU/ml) bound 40.1% ± 8.3% and 61.0% ± 6.0% of added AFB1 after 1 h, respectively. AFB1 caused significant reductions of 30.1% (P = 0.01), 49.4% (P = 0.004), and 64.4% (P < 0.001) in transepithelial resistance after 24, 48, and 72 h, respectively. Coincubation with 1 × 1010 CFU/ml GG after 24 h protected against AFB1-induced reductions in transepithelial resistance at both 24 h (P = 0.002) and 48 h (P = 0.04). DNA fragmentation was apparent in cells treated only with AFB1 cells but not in cells coincubated with either 1 × 1010 or 5 × 1010 CFU/ml GG. GG reduced AFB1 uptake and protected against both membrane and DNA damage in the Caco-2 model. These data are suggestive of a beneficial role of GG against dietary exposure to aflatoxin. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179004
ISSN
2015 Impact Factor: 3.823
2015 SCImago Journal Rankings: 1.891
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGratz, Sen_US
dc.contributor.authorWu, QKen_US
dc.contributor.authorElNezami, Hen_US
dc.contributor.authorJuvonen, ROen_US
dc.contributor.authorMykkänen, Hen_US
dc.contributor.authorTurner, PCen_US
dc.date.accessioned2012-12-19T09:51:21Z-
dc.date.available2012-12-19T09:51:21Z-
dc.date.issued2007en_US
dc.identifier.citationApplied And Environmental Microbiology, 2007, v. 73 n. 12, p. 3958-3964en_US
dc.identifier.issn0099-2240en_US
dc.identifier.urihttp://hdl.handle.net/10722/179004-
dc.description.abstractThe probiotic Lactobacillus rhamnosus GG is able to bind the potent hepatocarcinogen aflatoxin B1 (AFB1) and thus potentially restrict its rapid absorption from the intestine. In this study we investigated the potential of GG to reduce AFB1 availability in vitro in Caco-2 cells adapted to express cytochrome P-450 (CYP) 3A4, such that both transport and toxicity could be assessed. Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. AFB1 levels in culture medium were measured by high-performance liquid chromatography. In CYP 3A4-induced monolayers, AFB1 transport from the apical to the basolateral chamber was reduced from 11.1% ± 1.9% to 6.4% ± 2.5% (P = 0.019) and to 3.3% ± 1.8% (P = 0.002) within the first hour in monolayers coincubated with GG (1 × 1010 and 5 × 1010 CFU/ml, respectively). GG (1 × 1010 and 5 × 1010 CFU/ml) bound 40.1% ± 8.3% and 61.0% ± 6.0% of added AFB1 after 1 h, respectively. AFB1 caused significant reductions of 30.1% (P = 0.01), 49.4% (P = 0.004), and 64.4% (P < 0.001) in transepithelial resistance after 24, 48, and 72 h, respectively. Coincubation with 1 × 1010 CFU/ml GG after 24 h protected against AFB1-induced reductions in transepithelial resistance at both 24 h (P = 0.002) and 48 h (P = 0.04). DNA fragmentation was apparent in cells treated only with AFB1 cells but not in cells coincubated with either 1 × 1010 or 5 × 1010 CFU/ml GG. GG reduced AFB1 uptake and protected against both membrane and DNA damage in the Caco-2 model. These data are suggestive of a beneficial role of GG against dietary exposure to aflatoxin. Copyright © 2007, American Society for Microbiology. All Rights Reserved.en_US
dc.languageengen_US
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.subject.meshAflatoxin B1 - Metabolism - Toxicityen_US
dc.subject.meshBiological Transport - Physiologyen_US
dc.subject.meshCaco-2 Cellsen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshCytochrome P-450 Cyp3aen_US
dc.subject.meshCytochrome P-450 Enzyme System - Metabolismen_US
dc.subject.meshDna Fragmentation - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshLactobacillus Rhamnosus - Metabolismen_US
dc.subject.meshProbiotics - Metabolismen_US
dc.titleLactobacillus rhamnosus strain GG reduces aflatoxin B1 transport, metabolism, and toxicity in Caco-2 cellsen_US
dc.typeArticleen_US
dc.identifier.emailElNezami, H: elnezami@hkucc.hku.hken_US
dc.identifier.authorityElNezami, H=rp00694en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/AEM.02944-06en_US
dc.identifier.pmid17449679-
dc.identifier.scopuseid_2-s2.0-34250817463en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34250817463&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume73en_US
dc.identifier.issue12en_US
dc.identifier.spage3958en_US
dc.identifier.epage3964en_US
dc.identifier.isiWOS:000247394200022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGratz, S=9242677500en_US
dc.identifier.scopusauthoridWu, QK=36801000100en_US
dc.identifier.scopusauthoridElNezami, H=6603690577en_US
dc.identifier.scopusauthoridJuvonen, RO=7005331813en_US
dc.identifier.scopusauthoridMykkänen, H=7003915985en_US
dc.identifier.scopusauthoridTurner, PC=7402096074en_US

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