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Article: Solid-phase extraction-fluorimetric high performance liquid chromatographic determination of domoic acid in natural seawater mediated by an amorphous titania sorbent

TitleSolid-phase extraction-fluorimetric high performance liquid chromatographic determination of domoic acid in natural seawater mediated by an amorphous titania sorbent
Authors
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aca
Citation
Analytica Chimica Acta, 2007, v. 583 n. 1, p. 111-117 How to Cite?
AbstractThe feasibility of using sol-gel amorphous titania (TiO 2) as a solid-phase sorbent for the pre-concentration of domoic acid (DA), a potent amnesic shellfish poisoning (ASP) toxin, directly from seawater was explored. The sol-gel titania material is able to adsorb DA from seawater, via the formation of ester-linkage between the carboxylic moieties of DA and the Ti-OH groups on the sorbent surface, at low pH and desorb it at high pH. The chemisorption process is not significantly interfered by the seawater matrix. The optimum pH values for the adsorption and desorption of DA were found to be pH 4 and 11, respectively. The optimal sorbent loading for the batch-type solid-phase extraction of DA was 0.67 mg-TiO 2 ng-DA -1 and adsorption equilibrium was achieved in 2 h at room temperature. The desorbed DA in 500 μL of 0.1 M alkaline borate buffer can be directly derviatized by 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) in aqueous media for fluorimetric HPLC quantification. Analyte recovery, repeatability and detection limit of this titania SPE-fluorimetric HPLC determination are 89%, 6.2% and 120 pg-DA mL -1 (n = 7, P < 0.05), respectively, for a sample volume of 30 mL. This titania SPE technique should also be applicable to the pre-concentration of other polar carboxylate- and phosphonate-containing biomolecules and pharmaceuticals in complex and interfering environmental sample matrices. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178974
ISSN
2015 Impact Factor: 4.712
2015 SCImago Journal Rankings: 1.548
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, IOMen_US
dc.contributor.authorTsang, VWHen_US
dc.contributor.authorChu, KKen_US
dc.contributor.authorLeung, SKen_US
dc.contributor.authorLam, MHWen_US
dc.contributor.authorLau, TCen_US
dc.contributor.authorLam, PKSen_US
dc.contributor.authorWu, RSSen_US
dc.date.accessioned2012-12-19T09:51:11Z-
dc.date.available2012-12-19T09:51:11Z-
dc.date.issued2007en_US
dc.identifier.citationAnalytica Chimica Acta, 2007, v. 583 n. 1, p. 111-117en_US
dc.identifier.issn0003-2670en_US
dc.identifier.urihttp://hdl.handle.net/10722/178974-
dc.description.abstractThe feasibility of using sol-gel amorphous titania (TiO 2) as a solid-phase sorbent for the pre-concentration of domoic acid (DA), a potent amnesic shellfish poisoning (ASP) toxin, directly from seawater was explored. The sol-gel titania material is able to adsorb DA from seawater, via the formation of ester-linkage between the carboxylic moieties of DA and the Ti-OH groups on the sorbent surface, at low pH and desorb it at high pH. The chemisorption process is not significantly interfered by the seawater matrix. The optimum pH values for the adsorption and desorption of DA were found to be pH 4 and 11, respectively. The optimal sorbent loading for the batch-type solid-phase extraction of DA was 0.67 mg-TiO 2 ng-DA -1 and adsorption equilibrium was achieved in 2 h at room temperature. The desorbed DA in 500 μL of 0.1 M alkaline borate buffer can be directly derviatized by 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) in aqueous media for fluorimetric HPLC quantification. Analyte recovery, repeatability and detection limit of this titania SPE-fluorimetric HPLC determination are 89%, 6.2% and 120 pg-DA mL -1 (n = 7, P < 0.05), respectively, for a sample volume of 30 mL. This titania SPE technique should also be applicable to the pre-concentration of other polar carboxylate- and phosphonate-containing biomolecules and pharmaceuticals in complex and interfering environmental sample matrices. © 2006 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/acaen_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.subject.meshChromatography, High Pressure Liquid - Methodsen_US
dc.subject.meshIndicators And Reagentsen_US
dc.subject.meshKainic Acid - Analogs & Derivatives - Analysis - Isolation & Purificationen_US
dc.subject.meshMarine Toxins - Analysis - Isolation & Purificationen_US
dc.subject.meshPhase Transitionen_US
dc.subject.meshSeawater - Analysisen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.subject.meshTitaniumen_US
dc.titleSolid-phase extraction-fluorimetric high performance liquid chromatographic determination of domoic acid in natural seawater mediated by an amorphous titania sorbenten_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.aca.2006.09.063en_US
dc.identifier.pmid17386534-
dc.identifier.scopuseid_2-s2.0-33845955221en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845955221&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume583en_US
dc.identifier.issue1en_US
dc.identifier.spage111en_US
dc.identifier.epage117en_US
dc.identifier.isiWOS:000243705500016-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChan, IOM=9239010800en_US
dc.identifier.scopusauthoridTsang, VWH=8404299300en_US
dc.identifier.scopusauthoridChu, KK=35073739800en_US
dc.identifier.scopusauthoridLeung, SK=55168680900en_US
dc.identifier.scopusauthoridLam, MHW=7202630175en_US
dc.identifier.scopusauthoridLau, TC=7102222310en_US
dc.identifier.scopusauthoridLam, PKS=7202365776en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US

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