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Article: Demonstration of receptor binding properties of VP2 of very virulent strain infectious bursal disease virus on Vero cells

TitleDemonstration of receptor binding properties of VP2 of very virulent strain infectious bursal disease virus on Vero cells
Authors
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusres
Citation
Virus Research, 2007, v. 123 n. 1, p. 50-56 How to Cite?
AbstractTissue culture adaptation of infectious bursal disease virus (IBDV) results in alternation of three residues on its major capsid protein VP2 and these residues may engage in receptor binding. Although the key of successful infection of tissue culture adapted IBDV in tissue cultures was defined as the virus entering steps, mechanism of the adaptation is poorly understood. In this study, recombinant VP2s of an attenuated strain (D78) and a very virulent strain (HK46) of IBDV tagged with rabbit immunoglobulin G heavy chain were expressed in mammalian cells, generating RAVP2 and RVVP2, respectively, in high purity. Using flow cytometry, both RAVP2 and RVVP2 were demonstrated to bind with Vero cells while these bindings were blocked by D78 viral particles, implying both very virulent IBDVs (vvIBDVs) and attenuated IBDVs bind to Vero cells through the same receptor(s). Since vvIBDVs cannot be propagated directly in tissue cultures, the specific binding between RVVP2 and Vero cells suggests the barrier for tissue culture adaptation may be beyond the virus attachment process. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178968
ISSN
2015 Impact Factor: 2.526
2015 SCImago Journal Rankings: 1.259
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYip, CWen_US
dc.contributor.authorYeung, YSen_US
dc.contributor.authorMa, CMen_US
dc.contributor.authorLam, PYen_US
dc.contributor.authorHon, CCen_US
dc.contributor.authorZeng, Fen_US
dc.contributor.authorLeung, FCCen_US
dc.date.accessioned2012-12-19T09:51:08Z-
dc.date.available2012-12-19T09:51:08Z-
dc.date.issued2007en_US
dc.identifier.citationVirus Research, 2007, v. 123 n. 1, p. 50-56en_US
dc.identifier.issn0168-1702en_US
dc.identifier.urihttp://hdl.handle.net/10722/178968-
dc.description.abstractTissue culture adaptation of infectious bursal disease virus (IBDV) results in alternation of three residues on its major capsid protein VP2 and these residues may engage in receptor binding. Although the key of successful infection of tissue culture adapted IBDV in tissue cultures was defined as the virus entering steps, mechanism of the adaptation is poorly understood. In this study, recombinant VP2s of an attenuated strain (D78) and a very virulent strain (HK46) of IBDV tagged with rabbit immunoglobulin G heavy chain were expressed in mammalian cells, generating RAVP2 and RVVP2, respectively, in high purity. Using flow cytometry, both RAVP2 and RVVP2 were demonstrated to bind with Vero cells while these bindings were blocked by D78 viral particles, implying both very virulent IBDVs (vvIBDVs) and attenuated IBDVs bind to Vero cells through the same receptor(s). Since vvIBDVs cannot be propagated directly in tissue cultures, the specific binding between RVVP2 and Vero cells suggests the barrier for tissue culture adaptation may be beyond the virus attachment process. © 2006 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusresen_US
dc.relation.ispartofVirus Researchen_US
dc.rightsVirus Research. Copyright © Elsevier BV.-
dc.subject.meshAdaptation, Physiologicalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBirnaviridae Infections - Virologyen_US
dc.subject.meshCapsid Proteins - Metabolismen_US
dc.subject.meshCercopithecus Aethiopsen_US
dc.subject.meshInfectious Bursal Disease Virus - Chemistry - Physiologyen_US
dc.subject.meshReceptors, Virus - Metabolismen_US
dc.subject.meshVero Cellsen_US
dc.subject.meshVirus Replicationen_US
dc.titleDemonstration of receptor binding properties of VP2 of very virulent strain infectious bursal disease virus on Vero cellsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FCC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.virusres.2006.08.001en_US
dc.identifier.pmid16965830-
dc.identifier.scopuseid_2-s2.0-33751009642en_US
dc.identifier.hkuros133459-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33751009642&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume123en_US
dc.identifier.issue1en_US
dc.identifier.spage50en_US
dc.identifier.epage56en_US
dc.identifier.isiWOS:000243743900006-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridYip, CW=7101665559en_US
dc.identifier.scopusauthoridYeung, YS=9841205900en_US
dc.identifier.scopusauthoridMa, CM=15062956500en_US
dc.identifier.scopusauthoridLam, PY=36847488300en_US
dc.identifier.scopusauthoridHon, CC=7003617137en_US
dc.identifier.scopusauthoridZeng, F=7202911544en_US
dc.identifier.scopusauthoridLeung, FCC=7103078633en_US

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