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Article: Characterization of heat-induced aggregates of globulin from common buckwheat (Fagopyrum esculentum Moench)

TitleCharacterization of heat-induced aggregates of globulin from common buckwheat (Fagopyrum esculentum Moench)
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijbiomac
Citation
International Journal Of Biological Macromolecules, 2006, v. 39 n. 4-5, p. 201-209 How to Cite?
AbstractSome physicochemical properties and the microstructure of heat-induced aggregates of globulin from common buckwheat (Fagopyrum esculentum Moench) (BWG) formed at 100 °C in 0.01 M phosphate buffer containing 1.0 M NaCl, pH 7.4 were studied. Differential scanning calorimetric (DSC) analysis shows a re-distribution of native and extensively denatured proteins in the heat-induced aggregates of BWG, particularly in the ISA fraction. Sodium dodecyl sulfate polyacrylamide gel electrophoretic (SDS-PAGE) analysis suggests the occurrence of both dissociation and association of molecules and the involvement of intermolecular disulfide linkages during thermal aggregation. Transmission electron microscopy (TEM) reveals that native BWG appeared as uniform compact globules with diameters ranging between 11.7 and 12.5 nm. TEM examination of the buffer-soluble aggregates, fractionated by sucrose density gradient ultracentrifugation, demonstrates the formation of strand-like small aggregates and large compact globular soluble macroaggregates. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178963
ISSN
2015 Impact Factor: 3.138
2015 SCImago Journal Rankings: 0.815
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChoi, SMen_US
dc.contributor.authorMine, Yen_US
dc.contributor.authorMa, CYen_US
dc.date.accessioned2012-12-19T09:51:06Z-
dc.date.available2012-12-19T09:51:06Z-
dc.date.issued2006en_US
dc.identifier.citationInternational Journal Of Biological Macromolecules, 2006, v. 39 n. 4-5, p. 201-209en_US
dc.identifier.issn0141-8130en_US
dc.identifier.urihttp://hdl.handle.net/10722/178963-
dc.description.abstractSome physicochemical properties and the microstructure of heat-induced aggregates of globulin from common buckwheat (Fagopyrum esculentum Moench) (BWG) formed at 100 °C in 0.01 M phosphate buffer containing 1.0 M NaCl, pH 7.4 were studied. Differential scanning calorimetric (DSC) analysis shows a re-distribution of native and extensively denatured proteins in the heat-induced aggregates of BWG, particularly in the ISA fraction. Sodium dodecyl sulfate polyacrylamide gel electrophoretic (SDS-PAGE) analysis suggests the occurrence of both dissociation and association of molecules and the involvement of intermolecular disulfide linkages during thermal aggregation. Transmission electron microscopy (TEM) reveals that native BWG appeared as uniform compact globules with diameters ranging between 11.7 and 12.5 nm. TEM examination of the buffer-soluble aggregates, fractionated by sucrose density gradient ultracentrifugation, demonstrates the formation of strand-like small aggregates and large compact globular soluble macroaggregates. © 2006 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijbiomacen_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.rightsInternational Journal of Biological Macromolecules. Copyright © Elsevier BV.-
dc.subject.meshCalorimetry, Differential Scanningen_US
dc.subject.meshDisulfides - Chemistryen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFagopyrum - Chemistryen_US
dc.subject.meshGlobulins - Chemistryen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshMultiprotein Complexesen_US
dc.subject.meshPlant Proteins - Chemistryen_US
dc.subject.meshSulfhydryl Compounds - Chemistryen_US
dc.subject.meshThermodynamicsen_US
dc.subject.meshUltracentrifugationen_US
dc.titleCharacterization of heat-induced aggregates of globulin from common buckwheat (Fagopyrum esculentum Moench)en_US
dc.typeArticleen_US
dc.identifier.emailMa, CY: macy@hkucc.hku.hken_US
dc.identifier.authorityMa, CY=rp00759en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ijbiomac.2006.03.025en_US
dc.identifier.pmid16677704-
dc.identifier.scopuseid_2-s2.0-33750173897en_US
dc.identifier.hkuros128364-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750173897&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume39en_US
dc.identifier.issue4-5en_US
dc.identifier.spage201en_US
dc.identifier.epage209en_US
dc.identifier.isiWOS:000242525200008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChoi, SM=8873744400en_US
dc.identifier.scopusauthoridMine, Y=7103350429en_US
dc.identifier.scopusauthoridMa, CY=7402924944en_US

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