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Article: Extracellular serotonin (5-ht) levels in the lateral hypothalamus (lh) after tryptophan injection in normal, obese, weight reduced obese and diet resistant rats

TitleExtracellular serotonin (5-ht) levels in the lateral hypothalamus (lh) after tryptophan injection in normal, obese, weight reduced obese and diet resistant rats
Authors
Issue Date1996
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
Faseb Journal, 1996, v. 10 n. 3, p. A216 How to Cite?
AbstractExtracellular 5-HT level in the LH was examined using the in. vivo microdialysis technique. Female Spraguc-Dawlcy rats (100 gm) were given either a basal diet (5% fat) or a high (30%) fat diet. In two to three months, 75% of the rats fed the high fat diet became obese (gained more weight than the highest weight gainer of the low fat group). Half of the obese rats (DIO) were then switched to the low fat diet until their cumulative weight gain was less than that achieved by the highest weight gainer of the control group [weight reduced obese (WRO)]. Those rats remained lean on a high fat diet were diet resistant (DR). AU rats then received guide cannuU implantation. On test days, a dialysis probe (2 nun, perfused with artificial cerebrospinal fluid at 1.5 ul/min) was inserted through the implanted guide carmula 3 hours before treatment. Each rat, on separate days, received saline, tryptophan (75 mg/kg) or dexfenfluramine (2 mg/kg) intraperitoneally and perfusates (30 min aliquots) were collected for a period of 210 min. Serotonin and its metabolite 5-hydroxyindoleacetic acid were determined eleclrochcmically after HPLC separation. The two indole levels just before injection were averaged and taken as the baseline level for that day. Subsequent changes in level were expressed as percent of baseline. ANOVA with repeated measures revealed a significant interaction between rat group and treatment (p<0.05). Extracellular 5-HT levels in the LH increased after tryptophan hi both control and DR rats but not in DIO and WRO ran. These findings suggest obesity reduces central serotonergic tone and this effect persists even after the normalization of body weight in the DIO rats.
Persistent Identifierhttp://hdl.handle.net/10722/178958
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorLeann, WWLen_US
dc.contributor.authorTse, IMYen_US
dc.contributor.authorLi, ETSen_US
dc.date.accessioned2012-12-19T09:51:02Z-
dc.date.available2012-12-19T09:51:02Z-
dc.date.issued1996en_US
dc.identifier.citationFaseb Journal, 1996, v. 10 n. 3, p. A216en_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttp://hdl.handle.net/10722/178958-
dc.description.abstractExtracellular 5-HT level in the LH was examined using the in. vivo microdialysis technique. Female Spraguc-Dawlcy rats (100 gm) were given either a basal diet (5% fat) or a high (30%) fat diet. In two to three months, 75% of the rats fed the high fat diet became obese (gained more weight than the highest weight gainer of the low fat group). Half of the obese rats (DIO) were then switched to the low fat diet until their cumulative weight gain was less than that achieved by the highest weight gainer of the control group [weight reduced obese (WRO)]. Those rats remained lean on a high fat diet were diet resistant (DR). AU rats then received guide cannuU implantation. On test days, a dialysis probe (2 nun, perfused with artificial cerebrospinal fluid at 1.5 ul/min) was inserted through the implanted guide carmula 3 hours before treatment. Each rat, on separate days, received saline, tryptophan (75 mg/kg) or dexfenfluramine (2 mg/kg) intraperitoneally and perfusates (30 min aliquots) were collected for a period of 210 min. Serotonin and its metabolite 5-hydroxyindoleacetic acid were determined eleclrochcmically after HPLC separation. The two indole levels just before injection were averaged and taken as the baseline level for that day. Subsequent changes in level were expressed as percent of baseline. ANOVA with repeated measures revealed a significant interaction between rat group and treatment (p<0.05). Extracellular 5-HT levels in the LH increased after tryptophan hi both control and DR rats but not in DIO and WRO ran. These findings suggest obesity reduces central serotonergic tone and this effect persists even after the normalization of body weight in the DIO rats.en_US
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_US
dc.relation.ispartofFASEB Journalen_US
dc.titleExtracellular serotonin (5-ht) levels in the lateral hypothalamus (lh) after tryptophan injection in normal, obese, weight reduced obese and diet resistant ratsen_US
dc.typeArticleen_US
dc.identifier.emailLi, ETS: etsli@hku.hken_US
dc.identifier.authorityLi, ETS=rp00737en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33749101590en_US
dc.identifier.volume10en_US
dc.identifier.issue3en_US
dc.identifier.spageA216en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeann, WWL=14633790700en_US
dc.identifier.scopusauthoridTse, IMY=36832303000en_US
dc.identifier.scopusauthoridLi, ETS=14018169600en_US

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