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Article: Degradability of dimethyl terephthalate by Variovorax paradoxus T4 and Sphingomonas yanoikuyae DOS01 isolated from deep-ocean sediments

TitleDegradability of dimethyl terephthalate by Variovorax paradoxus T4 and Sphingomonas yanoikuyae DOS01 isolated from deep-ocean sediments
Authors
Issue Date2006
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
Citation
Ecotoxicology, 2006, v. 15 n. 6, p. 549-557 How to Cite?
AbstractTwo strains of bacteria were isolated from deep-ocean sediments of the South China Sea using enrichment culturing technique and they were identified as Sphingomonas yanoikuyae DOS01 (AY878409) and Variovorax paradoxus T4 (AY878410) based on 16S rRNA gene sequences. S. yanoikuyae DOS01 was only capable of transforming dimethyl terephthalate (DMTP) to monomethyl terephthalate (MMTP) without further degradation while V. paradoxus T4 exhibited ability in mineralizing DMTP as the sole source of carbon and energy. The biochemical pathway of DMTP degradation was through MMTP and terephthalic acid (TA) as major detectable degradation intermediates in the culture media by both microorganisms. V. paradoxus T4 utilized DMTP and MMTP via hydrolysis of diester and monoester in the initial steps in degradation as confirmed by total organic carbon analysis of the culture medium and esterase activity assay of the lysed cells and fraction. The specific hydrolysis activity of esterase induced by DMTP or MMTP showed that greater hydrolysis of p-nitrophenyl acetate by esterase induced by DMTP-grown cells than that induced by MMTP. Results of this research suggest that the cleavage of the two identical carboxylic ester groups of phthalate diester are carried out by highly specific esterases of the same bacteria in the environment. © Springer Science+Business Media, LLC 2006.
Persistent Identifierhttp://hdl.handle.net/10722/178957
ISSN
2015 Impact Factor: 2.329
2015 SCImago Journal Rankings: 1.108
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, YPen_US
dc.contributor.authorGu, JDen_US
dc.date.accessioned2012-12-19T09:51:02Z-
dc.date.available2012-12-19T09:51:02Z-
dc.date.issued2006en_US
dc.identifier.citationEcotoxicology, 2006, v. 15 n. 6, p. 549-557en_US
dc.identifier.issn0963-9292en_US
dc.identifier.urihttp://hdl.handle.net/10722/178957-
dc.description.abstractTwo strains of bacteria were isolated from deep-ocean sediments of the South China Sea using enrichment culturing technique and they were identified as Sphingomonas yanoikuyae DOS01 (AY878409) and Variovorax paradoxus T4 (AY878410) based on 16S rRNA gene sequences. S. yanoikuyae DOS01 was only capable of transforming dimethyl terephthalate (DMTP) to monomethyl terephthalate (MMTP) without further degradation while V. paradoxus T4 exhibited ability in mineralizing DMTP as the sole source of carbon and energy. The biochemical pathway of DMTP degradation was through MMTP and terephthalic acid (TA) as major detectable degradation intermediates in the culture media by both microorganisms. V. paradoxus T4 utilized DMTP and MMTP via hydrolysis of diester and monoester in the initial steps in degradation as confirmed by total organic carbon analysis of the culture medium and esterase activity assay of the lysed cells and fraction. The specific hydrolysis activity of esterase induced by DMTP or MMTP showed that greater hydrolysis of p-nitrophenyl acetate by esterase induced by DMTP-grown cells than that induced by MMTP. Results of this research suggest that the cleavage of the two identical carboxylic ester groups of phthalate diester are carried out by highly specific esterases of the same bacteria in the environment. © Springer Science+Business Media, LLC 2006.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292en_US
dc.relation.ispartofEcotoxicologyen_US
dc.subject.meshCarbon - Chemistryen_US
dc.subject.meshChemistry, Organic - Methodsen_US
dc.subject.meshGeologic Sedimentsen_US
dc.subject.meshHydrolysisen_US
dc.subject.meshKineticsen_US
dc.subject.meshModels, Chemicalen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNitrophenols - Chemistryen_US
dc.subject.meshOceans And Seasen_US
dc.subject.meshPhthalic Acids - Chemistry - Metabolismen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshProteobacteria - Metabolismen_US
dc.subject.meshRna, Ribosomal, 16S - Metabolismen_US
dc.subject.meshSoil Microbiologyen_US
dc.subject.meshSphingomonas - Metabolismen_US
dc.titleDegradability of dimethyl terephthalate by Variovorax paradoxus T4 and Sphingomonas yanoikuyae DOS01 isolated from deep-ocean sedimentsen_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s10646-006-0093-1en_US
dc.identifier.pmid16955363-
dc.identifier.scopuseid_2-s2.0-33748961325en_US
dc.identifier.hkuros134263-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748961325&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume15en_US
dc.identifier.issue6en_US
dc.identifier.spage549en_US
dc.identifier.epage557en_US
dc.identifier.isiWOS:000240719000007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWang, YP=9736272200en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US

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