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Article: Cloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer

TitleCloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer
Authors
Issue Date2006
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0923-9820
Citation
Biodegradation, 2006, v. 17 n. 5, p. 465-472 How to Cite?
AbstractSeven organophosphorus pesticide-degrading bacteria harboring the methyl parathion degrading (mpd) gene were isolated from a methyl parathion contaminated site. In this study, the 4.7 kb mpd gene cluster, conserved in all seven bacteria capable of degrading methyl parathion, was cloned and further analysis revealed that this cluster contained five ORFs and the mpd gene was associated with a mobile element, IS6100. In addition to mpd gene ORF and tnpA ORF, three other ORFs showed high homology to the permease component of ABC-type transport system, the general secretion pathway protein B, and the RNA polymerase sigma 70 factor, respectively. The mpd genes of these 7 strains were subcloned and expressed in E. coli, SDS-PAGE and zymogram analysis showed that two expression products of mpd genes in E. coli were found, but the one without signal peptide showed the hydrolytic activities. Our evidences collectively suggest that mpd gene cluster may be disseminated through horizontal gene transfer based on phylogenetic analysis of the cluster and their host bacterial strains, and comparisons of GC content of the cluster and respective host's chromosome. © Springer 2006.
Persistent Identifierhttp://hdl.handle.net/10722/178955
ISSN
2015 Impact Factor: 2.208
2015 SCImago Journal Rankings: 0.907
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ren_US
dc.contributor.authorCui, Zen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorJiang, Jen_US
dc.contributor.authorGu, JDen_US
dc.contributor.authorLi, Sen_US
dc.date.accessioned2012-12-19T09:51:01Z-
dc.date.available2012-12-19T09:51:01Z-
dc.date.issued2006en_US
dc.identifier.citationBiodegradation, 2006, v. 17 n. 5, p. 465-472en_US
dc.identifier.issn0923-9820en_US
dc.identifier.urihttp://hdl.handle.net/10722/178955-
dc.description.abstractSeven organophosphorus pesticide-degrading bacteria harboring the methyl parathion degrading (mpd) gene were isolated from a methyl parathion contaminated site. In this study, the 4.7 kb mpd gene cluster, conserved in all seven bacteria capable of degrading methyl parathion, was cloned and further analysis revealed that this cluster contained five ORFs and the mpd gene was associated with a mobile element, IS6100. In addition to mpd gene ORF and tnpA ORF, three other ORFs showed high homology to the permease component of ABC-type transport system, the general secretion pathway protein B, and the RNA polymerase sigma 70 factor, respectively. The mpd genes of these 7 strains were subcloned and expressed in E. coli, SDS-PAGE and zymogram analysis showed that two expression products of mpd genes in E. coli were found, but the one without signal peptide showed the hydrolytic activities. Our evidences collectively suggest that mpd gene cluster may be disseminated through horizontal gene transfer based on phylogenetic analysis of the cluster and their host bacterial strains, and comparisons of GC content of the cluster and respective host's chromosome. © Springer 2006.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0923-9820en_US
dc.relation.ispartofBiodegradationen_US
dc.subject.meshAryldialkylphosphatase - Geneticsen_US
dc.subject.meshBiodegradation, Environmentalen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshEnvironmental Pollutionen_US
dc.subject.meshEscherichia Coli - Geneticsen_US
dc.subject.meshGene Transfer, Horizontalen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshGram-Negative Aerobic Rods And Cocci - Enzymology - Genetics - Isolation & Purificationen_US
dc.subject.meshMethyl Parathion - Metabolismen_US
dc.subject.meshMultigene Familyen_US
dc.subject.meshOpen Reading Framesen_US
dc.subject.meshPesticides - Metabolismen_US
dc.titleCloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transferen_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s10532-005-9018-6en_US
dc.identifier.pmid16477356-
dc.identifier.scopuseid_2-s2.0-33748657366en_US
dc.identifier.hkuros134260-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748657366&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume17en_US
dc.identifier.issue5en_US
dc.identifier.spage465en_US
dc.identifier.epage472en_US
dc.identifier.isiWOS:000240395700008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhang, R=8612262700en_US
dc.identifier.scopusauthoridCui, Z=8678239700en_US
dc.identifier.scopusauthoridZhang, X=11639083000en_US
dc.identifier.scopusauthoridJiang, J=35240365600en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US
dc.identifier.scopusauthoridLi, S=24587265400en_US

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