File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Accumulation of recombinant SARS-CoV spike protein in plant cytosol and chloroplasts indicate potential for development of plant-derived oral vaccines

TitleAccumulation of recombinant SARS-CoV spike protein in plant cytosol and chloroplasts indicate potential for development of plant-derived oral vaccines
Authors
Issue Date2006
PublisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/
Citation
Experimental Biology And Medicine, 2006, v. 231 n. 8, p. 1346-1352 How to Cite?
AbstractPlants are promising candidates as bioreactors for the production of oral recombinant proteins in the biopharmaceutical industry. As an initial step toward provision of an oral vaccine against the severe acute respiratory syndrome coronavirus (SARS-CoV), we have expressed a partial spike (S) protein of SARS-CoV in the cytosol of nuclear-transformed plants and in the chloroplasts of plastid-transformed plants. In the construction of both nuclear and plastid transformation vectors, a 2-kilobase nucleotide sequence encoding amino acids 1-658 of the SARS-CoV spike protein (S1) was modified with nucleotide changes, but not amino acid changes, to optimize codon usage for expression in plants. To investigate the subcellular localization of S1 during transient expression in tobacco leaves, a translational fusion consisting of S1 and the green fluorescent protein (GFP) was generated. Following agroinfiltration of tobacco leaves, analysis by laser confocal scanning microscopy revealed that the S1:GFP fusion protein was localized to the cytosol. In stable transgenic tobacco plants and lettuce plants generated by Agrobacterium-mediated transformation, tobacco and lettuce leaves were observed to express the S1 at high levels from the Cauliflower Mosaic Virus 35S promoter with Northern blot analysis. When the S1 was expressed in transplastomic tobacco, S1 messenger RNA and its corresponding protein were detected on Northern and Western blot analyses, respectively. Our results demonstrate the feasibility of producing S1 in nuclear- and chloroplast-transformed plants, indicating its potential in subsequent development of a plant-derived and safe oral recombinant subunit vaccine against the SARS-CoV in edible plants. Copyright © 2006 by the Society for Experimental Biology and Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/178953
ISSN
2015 Impact Factor: 2.542
2015 SCImago Journal Rankings: 0.991
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HYen_US
dc.contributor.authorRamalingam, Sen_US
dc.contributor.authorChye, MLen_US
dc.date.accessioned2012-12-19T09:51:01Z-
dc.date.available2012-12-19T09:51:01Z-
dc.date.issued2006en_US
dc.identifier.citationExperimental Biology And Medicine, 2006, v. 231 n. 8, p. 1346-1352en_US
dc.identifier.issn1535-3702en_US
dc.identifier.urihttp://hdl.handle.net/10722/178953-
dc.description.abstractPlants are promising candidates as bioreactors for the production of oral recombinant proteins in the biopharmaceutical industry. As an initial step toward provision of an oral vaccine against the severe acute respiratory syndrome coronavirus (SARS-CoV), we have expressed a partial spike (S) protein of SARS-CoV in the cytosol of nuclear-transformed plants and in the chloroplasts of plastid-transformed plants. In the construction of both nuclear and plastid transformation vectors, a 2-kilobase nucleotide sequence encoding amino acids 1-658 of the SARS-CoV spike protein (S1) was modified with nucleotide changes, but not amino acid changes, to optimize codon usage for expression in plants. To investigate the subcellular localization of S1 during transient expression in tobacco leaves, a translational fusion consisting of S1 and the green fluorescent protein (GFP) was generated. Following agroinfiltration of tobacco leaves, analysis by laser confocal scanning microscopy revealed that the S1:GFP fusion protein was localized to the cytosol. In stable transgenic tobacco plants and lettuce plants generated by Agrobacterium-mediated transformation, tobacco and lettuce leaves were observed to express the S1 at high levels from the Cauliflower Mosaic Virus 35S promoter with Northern blot analysis. When the S1 was expressed in transplastomic tobacco, S1 messenger RNA and its corresponding protein were detected on Northern and Western blot analyses, respectively. Our results demonstrate the feasibility of producing S1 in nuclear- and chloroplast-transformed plants, indicating its potential in subsequent development of a plant-derived and safe oral recombinant subunit vaccine against the SARS-CoV in edible plants. Copyright © 2006 by the Society for Experimental Biology and Medicine.en_US
dc.languageengen_US
dc.publisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/en_US
dc.relation.ispartofExperimental Biology and Medicineen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Nucleus - Geneticsen_US
dc.subject.meshCell Transformation, Viralen_US
dc.subject.meshChloroplasts - Chemistry - Metabolismen_US
dc.subject.meshCytosol - Chemistry - Metabolismen_US
dc.subject.meshLettuce - Genetics - Virologyen_US
dc.subject.meshMembrane Glycoproteins - Biosynthesis - Genetics - Immunologyen_US
dc.subject.meshMicroscopy, Confocalen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshPlastids - Geneticsen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRecombinant Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshSars Virus - Genetics - Immunologyen_US
dc.subject.meshTobacco - Genetics - Virologyen_US
dc.subject.meshVaccines, Edible - Chemical Synthesisen_US
dc.subject.meshViral Envelope Proteins - Biosynthesis - Genetics - Immunologyen_US
dc.subject.meshViral Vaccines - Chemical Synthesisen_US
dc.titleAccumulation of recombinant SARS-CoV spike protein in plant cytosol and chloroplasts indicate potential for development of plant-derived oral vaccinesen_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid16946403en_US
dc.identifier.scopuseid_2-s2.0-33748313109en_US
dc.identifier.hkuros128820-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748313109&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume231en_US
dc.identifier.issue8en_US
dc.identifier.spage1346en_US
dc.identifier.epage1352en_US
dc.identifier.isiWOS:000240628100008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, HY=22953303900en_US
dc.identifier.scopusauthoridRamalingam, S=8709830400en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats