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Article: Downregulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed development

TitleDownregulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed development
Authors
Issue Date2006
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412
Citation
Plant Journal, 2006, v. 45 n. 1, p. 58-70 How to Cite?
AbstractProteinase inhibitor II proteins (PIN2) are serine proteinase inhibitors found in the Solanaceae. Here, we assign functions in seed development to two Solanum americanum genes, SaPIN2a and SaPIN2b, encoding proteinase inhibitor II. Their mRNAs and proteins have been previously localized to the reproductive tissues, including the inner cell layers of ovules in senescent flowers at the beginning of fruit development, suggestive of their endogenous roles in reproductive development. We have employed RNA interference (RNAi)-induced post-transcriptional gene silencing (PTGS) to further investigate the role of SaPIN2a and SaPIN2b during seed development. A SaPIN2a-derived construct that shared 83% nucleotide homology to SaPIN2b was used in PTGS to silence both genes. Northern blot analyses confirmed that the PIN2-RNAi transgenic plants contain small interfering RNAs (siRNAs) and exhibit reduced levels of SaPIN2a and SaPIN2b mRNAs at various stages of floral development. A reduction in seed set due to seed abortion was observed in PIN2-RNAi transgenic lines. Cytological and molecular analyses of these lines showed the lack of SaPIN2a and SaPIN2b mRNAs and proteins at the inner cell layers of the ovules in senescent flowers. Aborted seeds in transgenic fruits had an abnormal endothelium. The anomalous expansion of the endothelium prevented proper development of the endosperm and embryo, leading to seed abortion. Our observations indicate that SaPIN2a and SaPIN2b are essential for seed development and suggest that the endothelium may protect the embryo sac, allowing proper formation of the endosperm and embryo, as a result of its ability to produce proteinase inhibitors. © 2005 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/178932
ISSN
2015 Impact Factor: 5.468
2015 SCImago Journal Rankings: 3.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSin, SFen_US
dc.contributor.authorYeung, ECen_US
dc.contributor.authorChye, MLen_US
dc.date.accessioned2012-12-19T09:50:49Z-
dc.date.available2012-12-19T09:50:49Z-
dc.date.issued2006en_US
dc.identifier.citationPlant Journal, 2006, v. 45 n. 1, p. 58-70en_US
dc.identifier.issn0960-7412en_US
dc.identifier.urihttp://hdl.handle.net/10722/178932-
dc.description.abstractProteinase inhibitor II proteins (PIN2) are serine proteinase inhibitors found in the Solanaceae. Here, we assign functions in seed development to two Solanum americanum genes, SaPIN2a and SaPIN2b, encoding proteinase inhibitor II. Their mRNAs and proteins have been previously localized to the reproductive tissues, including the inner cell layers of ovules in senescent flowers at the beginning of fruit development, suggestive of their endogenous roles in reproductive development. We have employed RNA interference (RNAi)-induced post-transcriptional gene silencing (PTGS) to further investigate the role of SaPIN2a and SaPIN2b during seed development. A SaPIN2a-derived construct that shared 83% nucleotide homology to SaPIN2b was used in PTGS to silence both genes. Northern blot analyses confirmed that the PIN2-RNAi transgenic plants contain small interfering RNAs (siRNAs) and exhibit reduced levels of SaPIN2a and SaPIN2b mRNAs at various stages of floral development. A reduction in seed set due to seed abortion was observed in PIN2-RNAi transgenic lines. Cytological and molecular analyses of these lines showed the lack of SaPIN2a and SaPIN2b mRNAs and proteins at the inner cell layers of the ovules in senescent flowers. Aborted seeds in transgenic fruits had an abnormal endothelium. The anomalous expansion of the endothelium prevented proper development of the endosperm and embryo, leading to seed abortion. Our observations indicate that SaPIN2a and SaPIN2b are essential for seed development and suggest that the endothelium may protect the embryo sac, allowing proper formation of the endosperm and embryo, as a result of its ability to produce proteinase inhibitors. © 2005 The Authors.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412en_US
dc.relation.ispartofPlant Journalen_US
dc.rightsThe Plant Journal. Copyright © Blackwell Publishing Ltd.-
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshDna Primersen_US
dc.subject.meshGene Silencingen_US
dc.subject.meshGenes, Planten_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPlant Proteins - Geneticsen_US
dc.subject.meshRna Interferenceen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshRna, Small Interfering - Geneticsen_US
dc.subject.meshSeeds - Growth & Developmenten_US
dc.subject.meshSolanum - Embryology - Geneticsen_US
dc.titleDownregulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed developmenten_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1111/j.1365-313X.2005.02597.xen_US
dc.identifier.pmid16367954-
dc.identifier.scopuseid_2-s2.0-33644852137en_US
dc.identifier.hkuros113418-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644852137&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue1en_US
dc.identifier.spage58en_US
dc.identifier.epage70en_US
dc.identifier.isiWOS:000234129500005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSin, SF=7006553279en_US
dc.identifier.scopusauthoridYeung, EC=7101971768en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US

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