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Article: Chemical moieties and interactions involved in the binding of zearalenone to the surface of Lactobacillus rhamnosus strains GG

TitleChemical moieties and interactions involved in the binding of zearalenone to the surface of Lactobacillus rhamnosus strains GG
Authors
Issue Date2004
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 2004, v. 52 n. 14, p. 4577-4581 How to Cite?
AbstractViable, heat-and acid-killed Lactobacillus rhamnosus strain GG (LGG) has shown high binding properties with zearalenone (ZEN). To identify the type of chemical moieties and interactions involved in binding with the ZEN, LGG was subjected to different chemical and enzymatical treatments, prior to the binding experiments. Pretreating the viable, heat- and acid-killed bacteria with m-periodate significantly decreased ZEN binding, suggesting that ZEN binds predominantly to carbohydrate components. Pretreatment with Pronase E had no effect on the ability of viable cells to bind ZEN, however, a reduction in the binding of ZEN by heat- and acid-killed cells, suggesting that the new binding sites exposed by heat or acid are proteins in nature. Pretreatment with urea also decreased binding, suggesting that hydrophobic interactions play a role in ZEN binding. The binding of ZEN in concentrations ranging from 0.79 to 62.82 μM and its subsequent dissociation by repetitive aqueous washes was also studied. The binding sites of the bacteria were not saturated by the maximum ZEN concentration studied.
Persistent Identifierhttp://hdl.handle.net/10722/178926
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorElNezami, Hen_US
dc.contributor.authorPolychronaki, Nen_US
dc.contributor.authorLee, YKen_US
dc.contributor.authorHaskard, Cen_US
dc.contributor.authorJuvonen, Ren_US
dc.contributor.authorSalminen, Sen_US
dc.contributor.authorMykkänen, Hen_US
dc.date.accessioned2012-12-19T09:50:47Z-
dc.date.available2012-12-19T09:50:47Z-
dc.date.issued2004en_US
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2004, v. 52 n. 14, p. 4577-4581en_US
dc.identifier.issn0021-8561en_US
dc.identifier.urihttp://hdl.handle.net/10722/178926-
dc.description.abstractViable, heat-and acid-killed Lactobacillus rhamnosus strain GG (LGG) has shown high binding properties with zearalenone (ZEN). To identify the type of chemical moieties and interactions involved in binding with the ZEN, LGG was subjected to different chemical and enzymatical treatments, prior to the binding experiments. Pretreating the viable, heat- and acid-killed bacteria with m-periodate significantly decreased ZEN binding, suggesting that ZEN binds predominantly to carbohydrate components. Pretreatment with Pronase E had no effect on the ability of viable cells to bind ZEN, however, a reduction in the binding of ZEN by heat- and acid-killed cells, suggesting that the new binding sites exposed by heat or acid are proteins in nature. Pretreatment with urea also decreased binding, suggesting that hydrophobic interactions play a role in ZEN binding. The binding of ZEN in concentrations ranging from 0.79 to 62.82 μM and its subsequent dissociation by repetitive aqueous washes was also studied. The binding sites of the bacteria were not saturated by the maximum ZEN concentration studied.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_US
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_US
dc.subject.meshBacterial Proteins - Metabolismen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCarbohydrate Metabolismen_US
dc.subject.meshCarcinogens - Metabolismen_US
dc.subject.meshEstrogens, Non-Steroidal - Metabolismen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshLactobacillus - Chemistry - Metabolismen_US
dc.subject.meshUrea - Pharmacologyen_US
dc.subject.meshZearalenone - Chemistry - Metabolismen_US
dc.titleChemical moieties and interactions involved in the binding of zearalenone to the surface of Lactobacillus rhamnosus strains GGen_US
dc.typeArticleen_US
dc.identifier.emailElNezami, H: elnezami@hkucc.hku.hken_US
dc.identifier.authorityElNezami, H=rp00694en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/jf049924men_US
dc.identifier.pmid15237970-
dc.identifier.scopuseid_2-s2.0-3142615322en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3142615322&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume52en_US
dc.identifier.issue14en_US
dc.identifier.spage4577en_US
dc.identifier.epage4581en_US
dc.identifier.isiWOS:000222540800036-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridElNezami, H=6603690577en_US
dc.identifier.scopusauthoridPolychronaki, N=6505944468en_US
dc.identifier.scopusauthoridLee, YK=36472355300en_US
dc.identifier.scopusauthoridHaskard, C=6602356857en_US
dc.identifier.scopusauthoridJuvonen, R=7005331813en_US
dc.identifier.scopusauthoridSalminen, S=7102912002en_US
dc.identifier.scopusauthoridMykkänen, H=7003915985en_US

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