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Article: Cloning and characterization of a PAC1 receptor hop-1 splice variant in goldfish (Carassius auratus)

TitleCloning and characterization of a PAC1 receptor hop-1 splice variant in goldfish (Carassius auratus)
Authors
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2006, v. 145 n. 2, p. 188-196 How to Cite?
AbstractIn several vertebrates, it has been demonstrated that alternative splicing of PAC1 receptor (PAC1-R) transcripts can generate a number of functional receptor variants which utilize different signal transduction pathways to mediate their activities. As PACAP is a physiological growth hormone-releasing factor in fish, and PACAP and the PAC1-R are highly conserved in vertebrate evolution, it would be of interest to investigate the structure and cellular distribution, particularly in the pituitary, of PAC1-R splice variants in a fish model. Our laboratory has previously cloned a receptor cDNA corresponding to the goldfish PAC1-R-s (goldfish PAC1-R-short). In the present study, a goldfish PAC1-R-hop1 variant was characterized. Functional expression of goldfish PAC1-R-s and PAC1-R-hop1 in Chinese Hamster Ovary cells revealed that, upon stimulation by ovine PACAP38, these receptor variants exhibited similar EC 50 values (8.7 ± 1.5 and 8.8 ± 1.9 nM, respectively) and maximal responses in activating intracellular cAMP production. The presence and expression levels of these transcripts were measured by quantitative real-time PCR in the brain, heart, pituitary and male gonad, and goldfish PAC1-R-s were found to be the predominant form. In situ hybridization of goldfish PAC1-R in the pituitary revealed its prevalent presence in the pars distalis. In summary, the present study provides information to confirm the role of PACAP in the pituitary and to elucidate the pleiotropic effects of PACAP in fish. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178918
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
References
Grants

 

DC FieldValueLanguage
dc.contributor.authorKwok, YYen_US
dc.contributor.authorChu, JYSen_US
dc.contributor.authorVaudry, Hen_US
dc.contributor.authorYon, Len_US
dc.contributor.authorAnouar, Yen_US
dc.contributor.authorChow, BKCen_US
dc.date.accessioned2012-12-19T09:50:44Z-
dc.date.available2012-12-19T09:50:44Z-
dc.date.issued2006en_US
dc.identifier.citationGeneral And Comparative Endocrinology, 2006, v. 145 n. 2, p. 188-196en_US
dc.identifier.issn0016-6480en_US
dc.identifier.urihttp://hdl.handle.net/10722/178918-
dc.description.abstractIn several vertebrates, it has been demonstrated that alternative splicing of PAC1 receptor (PAC1-R) transcripts can generate a number of functional receptor variants which utilize different signal transduction pathways to mediate their activities. As PACAP is a physiological growth hormone-releasing factor in fish, and PACAP and the PAC1-R are highly conserved in vertebrate evolution, it would be of interest to investigate the structure and cellular distribution, particularly in the pituitary, of PAC1-R splice variants in a fish model. Our laboratory has previously cloned a receptor cDNA corresponding to the goldfish PAC1-R-s (goldfish PAC1-R-short). In the present study, a goldfish PAC1-R-hop1 variant was characterized. Functional expression of goldfish PAC1-R-s and PAC1-R-hop1 in Chinese Hamster Ovary cells revealed that, upon stimulation by ovine PACAP38, these receptor variants exhibited similar EC 50 values (8.7 ± 1.5 and 8.8 ± 1.9 nM, respectively) and maximal responses in activating intracellular cAMP production. The presence and expression levels of these transcripts were measured by quantitative real-time PCR in the brain, heart, pituitary and male gonad, and goldfish PAC1-R-s were found to be the predominant form. In situ hybridization of goldfish PAC1-R in the pituitary revealed its prevalent presence in the pars distalis. In summary, the present study provides information to confirm the role of PACAP in the pituitary and to elucidate the pleiotropic effects of PACAP in fish. © 2005 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_US
dc.relation.ispartofGeneral and Comparative Endocrinologyen_US
dc.subject.meshAlternative Splicingen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCho Cellsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshCricetulusen_US
dc.subject.meshDna, Complementary - Metabolismen_US
dc.subject.meshFish Proteins - Genetics - Physiologyen_US
dc.subject.meshGoldfish - Genetics - Metabolism - Physiologyen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptide - Physiologyen_US
dc.subject.meshPituitary Gland - Cytology - Metabolismen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshReceptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - Genetics - Physiologyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSignal Transductionen_US
dc.subject.meshTissue Distributionen_US
dc.titleCloning and characterization of a PAC1 receptor hop-1 splice variant in goldfish (Carassius auratus)en_US
dc.typeArticleen_US
dc.identifier.emailChu, JYS: hitan@graduate.hku.hken_US
dc.identifier.emailChow, BKC: bkcc@hku.hken_US
dc.identifier.authorityChu, JYS=rp00684en_US
dc.identifier.authorityChow, BKC=rp00681en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ygcen.2005.08.011en_US
dc.identifier.pmid16225877-
dc.identifier.scopuseid_2-s2.0-29344431653en_US
dc.identifier.hkuros115210-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-29344431653&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume145en_US
dc.identifier.issue2en_US
dc.identifier.spage188en_US
dc.identifier.epage196en_US
dc.identifier.isiWOS:000234616700012-
dc.publisher.placeUnited Statesen_US
dc.relation.projectFunctional and structural evolution of VIP and PACAP receptors in vertebrates-
dc.relation.projectThe concerted hypophysiotropic actions of GHRH and PACAP on the control of growth hormone release in goldfish, Carassius auratus-
dc.identifier.scopusauthoridKwok, YY=10243622600en_US
dc.identifier.scopusauthoridChu, JYS=34975209300en_US
dc.identifier.scopusauthoridVaudry, H=35446602600en_US
dc.identifier.scopusauthoridYon, L=6603856999en_US
dc.identifier.scopusauthoridAnouar, Y=7003561768en_US
dc.identifier.scopusauthoridChow, BKC=7102826193en_US

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