File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Polymorphisms of 5′ flanking region of chicken prolactin gene

TitlePolymorphisms of 5′ flanking region of chicken prolactin gene
Authors
Keywords5′ Flanking Region
Chicken Prolactin Gene
Gene Expression
Polymorphism
Issue Date2006
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/domaniend
Citation
Domestic Animal Endocrinology, 2006, v. 30 n. 1, p. 1-16 How to Cite?
AbstractThe polymorphisms of 5′ flanking region of chicken prolactin (cPRL) gene were examined in several populations of Chinese native Yuehuang, Taihe Silkie and imported White Leghorn Layer chickens. The 5′ flanking regions (2638 bp) from Yuehuang, Taihe Silkie and White Leghorn chickens were subjected to sequencing analysis. Four single nucleotide polymorphisms (SNPs) were identified at position -2425(C/T), -2215(T/C), -2063(G/A) and -1967(A/G). A 24-bp indel (insertion or deletion) and a polyA length polymorphism were also identified. For the 24-bp indel locus, three genotypes (AA, AB and BB) were found in Yuehuang chickens, while only two genotypes were detected in Taihe Silkie (AB and BB) and Leghorn chickens (AA and BB). The genotype frequencies of AA, AB and BB were significantly different among the three breeds. For the polyA locus, although three genotypes (CC, CD and DD) were found, only one genotype (CC) was detected in White Leghorn chickens, while two or three genotypes were observed in Chinese native chickens. We used real-time quantitative PCR and radioimmunology assay to investigate the potential association of the 24-bp indel locus with cPRL mRNA expression, plasma cPRL and brooding behaviors, and observed that chickens with genotype AB, which are of the highest incidence of broodiness, had the highest cPRL mRNA levels, providing the possibility that this polymorphic site might be related to the broodiness in chickens via modulating the transcriptional level of cPRL gene. The dissociation among cPRL gene transcription, mRNA storage and hormone release was also observed. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178912
ISSN
2015 Impact Factor: 1.613
2015 SCImago Journal Rankings: 0.751
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiang, Yen_US
dc.contributor.authorCui, Jen_US
dc.contributor.authorYang, Gen_US
dc.contributor.authorLeung, FCCen_US
dc.contributor.authorZhang, Xen_US
dc.date.accessioned2012-12-19T09:50:41Z-
dc.date.available2012-12-19T09:50:41Z-
dc.date.issued2006en_US
dc.identifier.citationDomestic Animal Endocrinology, 2006, v. 30 n. 1, p. 1-16en_US
dc.identifier.issn0739-7240en_US
dc.identifier.urihttp://hdl.handle.net/10722/178912-
dc.description.abstractThe polymorphisms of 5′ flanking region of chicken prolactin (cPRL) gene were examined in several populations of Chinese native Yuehuang, Taihe Silkie and imported White Leghorn Layer chickens. The 5′ flanking regions (2638 bp) from Yuehuang, Taihe Silkie and White Leghorn chickens were subjected to sequencing analysis. Four single nucleotide polymorphisms (SNPs) were identified at position -2425(C/T), -2215(T/C), -2063(G/A) and -1967(A/G). A 24-bp indel (insertion or deletion) and a polyA length polymorphism were also identified. For the 24-bp indel locus, three genotypes (AA, AB and BB) were found in Yuehuang chickens, while only two genotypes were detected in Taihe Silkie (AB and BB) and Leghorn chickens (AA and BB). The genotype frequencies of AA, AB and BB were significantly different among the three breeds. For the polyA locus, although three genotypes (CC, CD and DD) were found, only one genotype (CC) was detected in White Leghorn chickens, while two or three genotypes were observed in Chinese native chickens. We used real-time quantitative PCR and radioimmunology assay to investigate the potential association of the 24-bp indel locus with cPRL mRNA expression, plasma cPRL and brooding behaviors, and observed that chickens with genotype AB, which are of the highest incidence of broodiness, had the highest cPRL mRNA levels, providing the possibility that this polymorphic site might be related to the broodiness in chickens via modulating the transcriptional level of cPRL gene. The dissociation among cPRL gene transcription, mRNA storage and hormone release was also observed. © 2005 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/domanienden_US
dc.relation.ispartofDomestic Animal Endocrinologyen_US
dc.rightsDomestic Animal Endocrinology. Copyright © Elsevier Inc.-
dc.subject5′ Flanking Regionen_US
dc.subjectChicken Prolactin Geneen_US
dc.subjectGene Expressionen_US
dc.subjectPolymorphismen_US
dc.titlePolymorphisms of 5′ flanking region of chicken prolactin geneen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FCC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.domaniend.2005.05.006en_US
dc.identifier.pmid15970423-
dc.identifier.scopuseid_2-s2.0-27844455443en_US
dc.identifier.hkuros122984-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27844455443&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume30en_US
dc.identifier.issue1en_US
dc.identifier.spage1en_US
dc.identifier.epage16en_US
dc.identifier.isiWOS:000233827400001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLiang, Y=15061407700en_US
dc.identifier.scopusauthoridCui, J=55233270500en_US
dc.identifier.scopusauthoridYang, G=7405751413en_US
dc.identifier.scopusauthoridLeung, FCC=7103078633en_US
dc.identifier.scopusauthoridZhang, X=8439058600en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats