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Article: Expression of a microsporocyte-specific gene encoding dihydroflavonol 4-reductase-like protein is developmentally regulated during early microsporogenesis in rice

TitleExpression of a microsporocyte-specific gene encoding dihydroflavonol 4-reductase-like protein is developmentally regulated during early microsporogenesis in rice
Authors
KeywordsDihydroflavonol-4-Reductase (Dfr)
Male Sterility
Microsporocyte-Specific
Microsporogenesis
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00497/index.htm
Citation
Sexual Plant Reproduction, 2005, v. 18 n. 2, p. 65-74 How to Cite?
AbstractIn an attempt to understand more about the molecular mechanism by which pollen development is regulated and coordinated in rice, we employed a cDNA subtraction strategy to identify genes that differentially expressed between the wild-type, AN-N and its thermosensitive genic male-sterile mutant (TGMS), AN S-1 during microsporogenesis. Eleven clones exhibiting differential expression patterns were isolated and identified. We report here in detail the molecular cloning and characterization of one of the clones, OS-DFR2 which encodes a dihydroflavonol 4-reductase-like protein. RNA gel blot analysis revealed that OS-DFR2 expressed strongly in anthers but no expression was detected in roots, leaves, stems and pistils, indicating that OS-DFR2 was a tissue-specific gene. Furthermore, the expression of OS-DFR2 was developmentally regulated during early microsporogenesis. It accumulated at high levels in anthers undergoing meiosis and reached a maximal value during tetrad formation stage in the wild-type, AN-N. In AN S-1, the expression of OS-DFR2 resembled that of AN-N except its mRNA level is approximately one-fourth of that found in AN-N at the tetrad formation stage. In situ analysis indicated that OS-DFR2 transcript was specifically expressed in microsporocyte of AN-N; whereas OS-DFR2 was present both in microsporocyte and tapetal cells in AN S-1. The significance and possible role played by OS-DFR2 during male gametophyte development in rice is discussed. © Springer-Verlag 2005.
Persistent Identifierhttp://hdl.handle.net/10722/178907
ISSN
2014 Impact Factor: 0.931
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYau, CPen_US
dc.contributor.authorZhuang, CXen_US
dc.contributor.authorZee, SYen_US
dc.contributor.authorYip, WKen_US
dc.date.accessioned2012-12-19T09:50:38Z-
dc.date.available2012-12-19T09:50:38Z-
dc.date.issued2005en_US
dc.identifier.citationSexual Plant Reproduction, 2005, v. 18 n. 2, p. 65-74en_US
dc.identifier.issn0934-0882en_US
dc.identifier.urihttp://hdl.handle.net/10722/178907-
dc.description.abstractIn an attempt to understand more about the molecular mechanism by which pollen development is regulated and coordinated in rice, we employed a cDNA subtraction strategy to identify genes that differentially expressed between the wild-type, AN-N and its thermosensitive genic male-sterile mutant (TGMS), AN S-1 during microsporogenesis. Eleven clones exhibiting differential expression patterns were isolated and identified. We report here in detail the molecular cloning and characterization of one of the clones, OS-DFR2 which encodes a dihydroflavonol 4-reductase-like protein. RNA gel blot analysis revealed that OS-DFR2 expressed strongly in anthers but no expression was detected in roots, leaves, stems and pistils, indicating that OS-DFR2 was a tissue-specific gene. Furthermore, the expression of OS-DFR2 was developmentally regulated during early microsporogenesis. It accumulated at high levels in anthers undergoing meiosis and reached a maximal value during tetrad formation stage in the wild-type, AN-N. In AN S-1, the expression of OS-DFR2 resembled that of AN-N except its mRNA level is approximately one-fourth of that found in AN-N at the tetrad formation stage. In situ analysis indicated that OS-DFR2 transcript was specifically expressed in microsporocyte of AN-N; whereas OS-DFR2 was present both in microsporocyte and tapetal cells in AN S-1. The significance and possible role played by OS-DFR2 during male gametophyte development in rice is discussed. © Springer-Verlag 2005.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00497/index.htmen_US
dc.relation.ispartofSexual Plant Reproductionen_US
dc.subjectDihydroflavonol-4-Reductase (Dfr)en_US
dc.subjectMale Sterilityen_US
dc.subjectMicrosporocyte-Specificen_US
dc.subjectMicrosporogenesisen_US
dc.titleExpression of a microsporocyte-specific gene encoding dihydroflavonol 4-reductase-like protein is developmentally regulated during early microsporogenesis in riceen_US
dc.typeArticleen_US
dc.identifier.emailYip, WK: wkyip@hkucc.hku.hken_US
dc.identifier.authorityYip, WK=rp00833en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00497-005-0251-2en_US
dc.identifier.scopuseid_2-s2.0-26644444107en_US
dc.identifier.hkuros122848-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-26644444107&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume18en_US
dc.identifier.issue2en_US
dc.identifier.spage65en_US
dc.identifier.epage74en_US
dc.identifier.isiWOS:000232559700003-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridYau, CP=7007038455en_US
dc.identifier.scopusauthoridZhuang, CX=7003322231en_US
dc.identifier.scopusauthoridZee, SY=7004001733en_US
dc.identifier.scopusauthoridYip, WK=7102784428en_US
dc.identifier.citeulike8820882-

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