File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Analysis of artemisinin in Artemisia annua L. by LC-MS with selected ion monitoring

TitleAnalysis of artemisinin in Artemisia annua L. by LC-MS with selected ion monitoring
Authors
Issue Date2005
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 2005, v. 53 n. 18, p. 7010-7013 How to Cite?
AbstractA liquid chromatography-mass spectrometry (LC-MS) method with selected ion monitoring (SIM) was developed and validated for the analysis and standardization of artemisinin in Artemisia annua L. This method is simple and accurate and requires only an 11 min per sample running time for the direct detection and quantification of artemisinin, a sesquiterpene lactone with antimalarial activity. To accurately analyze artemisinin, SIM was used to record the abundance of the [M - 18 + H] + ion peak at m/z 265.3, with a scan range between m/z 250 and 270. Quantification was based on the LC-MS peak area of artemisinin, and the standard curve was used for calculation. This method was then validated and applied to several populations of A. annua to assess the population means and the diversity within a selected population. The distribution of artemisinin was found to vary by plant origin with population means ranging from 0.03 to 0.71% artemisinin dry leaf weight. Individual plants within the selected population that exhibited the highest artemisinin mean were then sampled, and the contents ranged from trace amounts to 1.5% artemisinin dry leaf weight basis, making these single plants accumulating artemisinin in concentrations >1.0% promising candidates for plant breeding and varietal development for high-yielding artemisinin. © 2005 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/178903
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Men_US
dc.contributor.authorPark, Cen_US
dc.contributor.authorWu, Qen_US
dc.contributor.authorSimon, JEen_US
dc.date.accessioned2012-12-19T09:50:36Z-
dc.date.available2012-12-19T09:50:36Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2005, v. 53 n. 18, p. 7010-7013en_US
dc.identifier.issn0021-8561en_US
dc.identifier.urihttp://hdl.handle.net/10722/178903-
dc.description.abstractA liquid chromatography-mass spectrometry (LC-MS) method with selected ion monitoring (SIM) was developed and validated for the analysis and standardization of artemisinin in Artemisia annua L. This method is simple and accurate and requires only an 11 min per sample running time for the direct detection and quantification of artemisinin, a sesquiterpene lactone with antimalarial activity. To accurately analyze artemisinin, SIM was used to record the abundance of the [M - 18 + H] + ion peak at m/z 265.3, with a scan range between m/z 250 and 270. Quantification was based on the LC-MS peak area of artemisinin, and the standard curve was used for calculation. This method was then validated and applied to several populations of A. annua to assess the population means and the diversity within a selected population. The distribution of artemisinin was found to vary by plant origin with population means ranging from 0.03 to 0.71% artemisinin dry leaf weight. Individual plants within the selected population that exhibited the highest artemisinin mean were then sampled, and the contents ranged from trace amounts to 1.5% artemisinin dry leaf weight basis, making these single plants accumulating artemisinin in concentrations >1.0% promising candidates for plant breeding and varietal development for high-yielding artemisinin. © 2005 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_US
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_US
dc.subject.meshAntimalarials - Analysisen_US
dc.subject.meshArtemisia - Chemistryen_US
dc.subject.meshArtemisinins - Analysisen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshChromatography, Liquid - Methodsen_US
dc.subject.meshMass Spectrometry - Methodsen_US
dc.subject.meshPlant Leaves - Chemistryen_US
dc.subject.meshSesquiterpenes - Analysisen_US
dc.titleAnalysis of artemisinin in Artemisia annua L. by LC-MS with selected ion monitoringen_US
dc.typeArticleen_US
dc.identifier.emailWang, M: mfwang@hku.hken_US
dc.identifier.authorityWang, M=rp00800en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/jf051061pen_US
dc.identifier.pmid16131103-
dc.identifier.scopuseid_2-s2.0-25444445012en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-25444445012&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume53en_US
dc.identifier.issue18en_US
dc.identifier.spage7010en_US
dc.identifier.epage7013en_US
dc.identifier.isiWOS:000231643700008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWang, M=7406691844en_US
dc.identifier.scopusauthoridPark, C=37047621000en_US
dc.identifier.scopusauthoridWu, Q=7404602467en_US
dc.identifier.scopusauthoridSimon, JE=7403956069en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats