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Article: Lack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus)

TitleLack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus)
Authors
KeywordsGrowth Hormone
Growth Hormone-Releasing Peptide-6
Hexarelin
Juvenile Grass Carp
Perifusion
Pituitary Cells
Pituitary Fragments
Static Incubation
Issue Date2002
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742
Citation
Fish Physiology And Biochemistry, 2002, v. 26 n. 4, p. 315-327 How to Cite?
AbstractStudies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. In the present study, GHRP-6 action on GH secretion was examined in vivo and in vitro in sexually immature grass carp. GHRP-6 injected intraperitoneally had no influences on serum GH levels in juvenile grass carp. Following intraperitonal injection of GHRP-6 and dopamine (DA) or cysteamine hydrochloride (CSH), alone and in combination into juvenile grass carp, DA and CSH were effective in elevating serum GH levels, but GHRP-6 was not effective in this respect; in addition, the synergistic action of GHRP-6 and DA or CSH on GH secretion was not seen. In this work, we had adapted and validated a perifusion system and a culture system for GH regulation studies. In a perifusion system, GHRP-6 (1000 to 0.1 nM), GHRP-6 (0.1 to 1000 nM), GHRP-6 (1 μM), and Hexarelin (an analog of GHRP, 1 μM) had no action on GH release from juvenile grass carp pituitary fragments or cells. Under static incubation conditions, GHRP-6 was inactive on GH release from juvenile grass carp pituitary fragments after 1 h and 6 h incubation, but human growth hormone-releasing hormone (hGHRH; 1 to 100 nM) as positive control could stimulate GH release in a dose-dependent manner. Furthermore, when GHRP-6 (100 nM) in combination static incubation with neuropeptides [e.g., hGHRH (100 nM), salmon gonadotropin-releasing hormone analogue (sGnRH-A) (100 nM), or D-Ala 6,Pro 9- NEt-luteinizing hormone-releasing hormone (D-Ala 6,Pro 6-NEt-LHRH, LHRH-A) (100nM)], GHRP-6 did not strengthen GH secretion actions of neuropeptides, and at the same time neuropeptides also did not modify the effects of GHRP-6 on GH secretion. The present results obtained using in vivo and in vitro techniques adapted for GH regulation studies show that GHRP-6 does not function as a GH-releasing factor in juvenile grass carp as it does in tilapia, amphibians, chickens, and mammals.
Persistent Identifierhttp://hdl.handle.net/10722/178872
ISSN
2015 Impact Factor: 1.442
2015 SCImago Journal Rankings: 0.738
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Den_US
dc.contributor.authorWong, AOLen_US
dc.contributor.authorLin, HRen_US
dc.date.accessioned2012-12-19T09:50:17Z-
dc.date.available2012-12-19T09:50:17Z-
dc.date.issued2002en_US
dc.identifier.citationFish Physiology And Biochemistry, 2002, v. 26 n. 4, p. 315-327en_US
dc.identifier.issn0920-1742en_US
dc.identifier.urihttp://hdl.handle.net/10722/178872-
dc.description.abstractStudies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. In the present study, GHRP-6 action on GH secretion was examined in vivo and in vitro in sexually immature grass carp. GHRP-6 injected intraperitoneally had no influences on serum GH levels in juvenile grass carp. Following intraperitonal injection of GHRP-6 and dopamine (DA) or cysteamine hydrochloride (CSH), alone and in combination into juvenile grass carp, DA and CSH were effective in elevating serum GH levels, but GHRP-6 was not effective in this respect; in addition, the synergistic action of GHRP-6 and DA or CSH on GH secretion was not seen. In this work, we had adapted and validated a perifusion system and a culture system for GH regulation studies. In a perifusion system, GHRP-6 (1000 to 0.1 nM), GHRP-6 (0.1 to 1000 nM), GHRP-6 (1 μM), and Hexarelin (an analog of GHRP, 1 μM) had no action on GH release from juvenile grass carp pituitary fragments or cells. Under static incubation conditions, GHRP-6 was inactive on GH release from juvenile grass carp pituitary fragments after 1 h and 6 h incubation, but human growth hormone-releasing hormone (hGHRH; 1 to 100 nM) as positive control could stimulate GH release in a dose-dependent manner. Furthermore, when GHRP-6 (100 nM) in combination static incubation with neuropeptides [e.g., hGHRH (100 nM), salmon gonadotropin-releasing hormone analogue (sGnRH-A) (100 nM), or D-Ala 6,Pro 9- NEt-luteinizing hormone-releasing hormone (D-Ala 6,Pro 6-NEt-LHRH, LHRH-A) (100nM)], GHRP-6 did not strengthen GH secretion actions of neuropeptides, and at the same time neuropeptides also did not modify the effects of GHRP-6 on GH secretion. The present results obtained using in vivo and in vitro techniques adapted for GH regulation studies show that GHRP-6 does not function as a GH-releasing factor in juvenile grass carp as it does in tilapia, amphibians, chickens, and mammals.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742en_US
dc.relation.ispartofFish Physiology and Biochemistryen_US
dc.subjectGrowth Hormoneen_US
dc.subjectGrowth Hormone-Releasing Peptide-6en_US
dc.subjectHexarelinen_US
dc.subjectJuvenile Grass Carpen_US
dc.subjectPerifusionen_US
dc.subjectPituitary Cellsen_US
dc.subjectPituitary Fragmentsen_US
dc.subjectStatic Incubationen_US
dc.titleLack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus)en_US
dc.typeArticleen_US
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AOL=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/B:FISH.0000009260.34930.12en_US
dc.identifier.scopuseid_2-s2.0-1642558448en_US
dc.identifier.hkuros113616-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1642558448&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume26en_US
dc.identifier.issue4en_US
dc.identifier.spage315en_US
dc.identifier.epage327en_US
dc.identifier.isiWOS:000187354100002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridXiao, D=45561966600en_US
dc.identifier.scopusauthoridWong, AOL=7403147570en_US
dc.identifier.scopusauthoridLin, HR=7405571313en_US

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