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Article: Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed counter-current chromatography

TitleSimultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed counter-current chromatography
Authors
Issue Date2005
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jss
Citation
Journal Of Separation Science, 2005, v. 28 n. 3, p. 268-272 How to Cite?
AbstractCnidium monnieri (L.) Cusson is a well-known Chinese medicinal plant, which has been used for the treatment of impotence, frigidity, and skin-related diseases, and exhibits strong antipruritic, antiallergic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities. A high-speed counter-current chromatography method was developed for the separation and purification of five bioactive coumarins from this plant. The crude coumarins were obtained by ethanol extraction from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. High-speed counter-current chromatography with the two-phase solvent systems n-hexane-ethyl acetate-ethanol-water (5:5:4:6, v/v) and n-hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v) was successfully performed with stepwise elution. The five relatively pure coumarins were obtained from 500 mg of the crude extract in a single run. Their purities were 90.6-98.9%, and the recoveries were 85.7-94.2%. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/178867
ISSN
2015 Impact Factor: 2.741
2015 SCImago Journal Rankings: 1.041
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:50:16Z-
dc.date.available2012-12-19T09:50:16Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Separation Science, 2005, v. 28 n. 3, p. 268-272en_US
dc.identifier.issn1615-9306en_US
dc.identifier.urihttp://hdl.handle.net/10722/178867-
dc.description.abstractCnidium monnieri (L.) Cusson is a well-known Chinese medicinal plant, which has been used for the treatment of impotence, frigidity, and skin-related diseases, and exhibits strong antipruritic, antiallergic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities. A high-speed counter-current chromatography method was developed for the separation and purification of five bioactive coumarins from this plant. The crude coumarins were obtained by ethanol extraction from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. High-speed counter-current chromatography with the two-phase solvent systems n-hexane-ethyl acetate-ethanol-water (5:5:4:6, v/v) and n-hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v) was successfully performed with stepwise elution. The five relatively pure coumarins were obtained from 500 mg of the crude extract in a single run. Their purities were 90.6-98.9%, and the recoveries were 85.7-94.2%. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jssen_US
dc.relation.ispartofJournal of Separation Scienceen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshCnidium - Chemistryen_US
dc.subject.meshCoumarins - Chemistry - Isolation & Purificationen_US
dc.subject.meshDrugs, Chinese Herbal - Chemistry - Isolation & Purificationen_US
dc.subject.meshMolecular Structureen_US
dc.subject.meshPlant Extracts - Chemistryen_US
dc.subject.meshPlants, Medicinal - Chemistryen_US
dc.subject.meshSolventsen_US
dc.titleSimultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed counter-current chromatographyen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jssc.200400002en_US
dc.identifier.pmid15776929-
dc.identifier.scopuseid_2-s2.0-15944373151en_US
dc.identifier.hkuros104953-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15944373151&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume28en_US
dc.identifier.issue3en_US
dc.identifier.spage268en_US
dc.identifier.epage272en_US
dc.identifier.isiWOS:000227397400008-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLi, HB=25649944400en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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