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Article: Monoclonal antibodies against apple 1-aminocyclopropane-l-carboxylate synthase

TitleMonoclonal antibodies against apple 1-aminocyclopropane-l-carboxylate synthase
Authors
Keywords1-Aminocyclopropane-L-Carboxylate Synthase
Ethylene
Fruit Ripening
Malus Sylvestris Mill
Monoclonal Antibody
Issue Date1991
PublisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/
Citation
Plant And Cell Physiology, 1991, v. 32 n. 1, p. 25-31 How to Cite?
Abstract1-Aminocyclopropane-l-carboxylate (ACC) synthase from apple fruits was purified over 5,000-fold by conventional column chromatography. By immunizing mice with this partially purified enzyme preparation, 8 hybridoma lines producing monoclonal antibodies against apple ACC synthase were isolated. While all 8 clones immunoprecipitated native ACC synthase, only two clones recognized the putative (48 kDa) ACC synthase on Western blots. When a partially purified ACC synthase preparation was incubated with S-adenosyl-L-[carboxyl- 14C]methionine (AdoMet), only one radioactive protein of 48 kDa was detected on sodium dodecyl sulfate-poly-acrylamide gel electrophoresis. This radioactive protein was specifically immunoprecipitated by the monoclonal antibodies, indicating that apple ACC synthase is specifically radiolabeled by its substrate AdoMet, as is tomato ACC synthase. Thus, the monoclonal antibodies recognized both native and AdoMet-inactivated forms of ACC synthase. While these antibodies failed to im-munoprecipitate ACC synthase isolated from ripe tomato fruits, ripe avocado fruits or auxin-treated mungbean hypocotyls, they were effective in immunoprecipitating the enzyme isolated from ripe pear fruits. © 1991 The Japanese Society of Plant Physilogists(JSPP).
Persistent Identifierhttp://hdl.handle.net/10722/178825
ISSN
2015 Impact Factor: 4.319
2015 SCImago Journal Rankings: 2.301

 

DC FieldValueLanguage
dc.contributor.authorDong, JGen_US
dc.contributor.authorYip, WKen_US
dc.contributor.authorYang, SFen_US
dc.date.accessioned2012-12-19T09:49:57Z-
dc.date.available2012-12-19T09:49:57Z-
dc.date.issued1991en_US
dc.identifier.citationPlant And Cell Physiology, 1991, v. 32 n. 1, p. 25-31en_US
dc.identifier.issn0032-0781en_US
dc.identifier.urihttp://hdl.handle.net/10722/178825-
dc.description.abstract1-Aminocyclopropane-l-carboxylate (ACC) synthase from apple fruits was purified over 5,000-fold by conventional column chromatography. By immunizing mice with this partially purified enzyme preparation, 8 hybridoma lines producing monoclonal antibodies against apple ACC synthase were isolated. While all 8 clones immunoprecipitated native ACC synthase, only two clones recognized the putative (48 kDa) ACC synthase on Western blots. When a partially purified ACC synthase preparation was incubated with S-adenosyl-L-[carboxyl- 14C]methionine (AdoMet), only one radioactive protein of 48 kDa was detected on sodium dodecyl sulfate-poly-acrylamide gel electrophoresis. This radioactive protein was specifically immunoprecipitated by the monoclonal antibodies, indicating that apple ACC synthase is specifically radiolabeled by its substrate AdoMet, as is tomato ACC synthase. Thus, the monoclonal antibodies recognized both native and AdoMet-inactivated forms of ACC synthase. While these antibodies failed to im-munoprecipitate ACC synthase isolated from ripe tomato fruits, ripe avocado fruits or auxin-treated mungbean hypocotyls, they were effective in immunoprecipitating the enzyme isolated from ripe pear fruits. © 1991 The Japanese Society of Plant Physilogists(JSPP).en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://pcp.oxfordjournals.org/en_US
dc.relation.ispartofPlant and Cell Physiologyen_US
dc.subject1-Aminocyclopropane-L-Carboxylate Synthaseen_US
dc.subjectEthyleneen_US
dc.subjectFruit Ripeningen_US
dc.subjectMalus Sylvestris Millen_US
dc.subjectMonoclonal Antibodyen_US
dc.titleMonoclonal antibodies against apple 1-aminocyclopropane-l-carboxylate synthaseen_US
dc.typeArticleen_US
dc.identifier.emailYip, WK: wkyip@hkucc.hku.hken_US
dc.identifier.authorityYip, WK=rp00833en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0343695462en_US
dc.identifier.volume32en_US
dc.identifier.issue1en_US
dc.identifier.spage25en_US
dc.identifier.epage31en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridDong, JG=55477984500en_US
dc.identifier.scopusauthoridYip, WK=7102784428en_US
dc.identifier.scopusauthoridYang, SF=16479591400en_US

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