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Article: Determination of isoflavones in red clover and related species by high-performance liquid chromatography combined with ultraviolet and mass spectrometric detection

TitleDetermination of isoflavones in red clover and related species by high-performance liquid chromatography combined with ultraviolet and mass spectrometric detection
Authors
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2003, v. 1016 n. 2, p. 195-209 How to Cite?
AbstractHigh-performance liquid chromatography-UV-electrospray ionization-mass spectrometric detector (HPLC-UV-ESI-MSD) method for determination of isoflavones in red clover (Trifolium pratense L.) and related species has been developed. The separated isoflavones including aglycones, glycosides and glycoside malonates, were individually analyzed and identified by their molecular ions and characteristic fragment ion peaks using LC-MSD under MS and MS-MS mode, and in comparison with the standard isoflavones. A total of 31 isoflavones were detected in red clover. Several isoflavones were also identified for the first time in related species, T. repense L. (white clover), T. hybridum L. (alsike clover) and T. campestre Schreber (hop trefoil). Based on reversed phase HPLC, all 10 isoflavone aglycones, daidzein, formononetin, genistein, pseudobaptigenin, glycitein, calycosin, prunetin, biochanin A, irilone and pratensein in acidic hydrolyzed extracts were successfully separated within 40min and quantified individually by UV and MS detectors. For the 10 target compounds, the investigated concentrations ranged from ∼24 to ∼12500ng/ml for UV detection and ∼6 to ∼3125ng/ml for MS detection, and good linearities (r2>0.999 for UV and r2>0.99 for MS) for standard curves were achieved for each isoflavone. The accuracy and repeatability (n=10) were within 15% for these 10 compounds. This is the first method reported that enables the simultaneous quantitation of all 10 isoflavone aglycones in red clover and related species. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178815
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, Qen_US
dc.contributor.authorWang, Men_US
dc.contributor.authorSimon, JEen_US
dc.date.accessioned2012-12-19T09:49:54Z-
dc.date.available2012-12-19T09:49:54Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Chromatography A, 2003, v. 1016 n. 2, p. 195-209en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://hdl.handle.net/10722/178815-
dc.description.abstractHigh-performance liquid chromatography-UV-electrospray ionization-mass spectrometric detector (HPLC-UV-ESI-MSD) method for determination of isoflavones in red clover (Trifolium pratense L.) and related species has been developed. The separated isoflavones including aglycones, glycosides and glycoside malonates, were individually analyzed and identified by their molecular ions and characteristic fragment ion peaks using LC-MSD under MS and MS-MS mode, and in comparison with the standard isoflavones. A total of 31 isoflavones were detected in red clover. Several isoflavones were also identified for the first time in related species, T. repense L. (white clover), T. hybridum L. (alsike clover) and T. campestre Schreber (hop trefoil). Based on reversed phase HPLC, all 10 isoflavone aglycones, daidzein, formononetin, genistein, pseudobaptigenin, glycitein, calycosin, prunetin, biochanin A, irilone and pratensein in acidic hydrolyzed extracts were successfully separated within 40min and quantified individually by UV and MS detectors. For the 10 target compounds, the investigated concentrations ranged from ∼24 to ∼12500ng/ml for UV detection and ∼6 to ∼3125ng/ml for MS detection, and good linearities (r2>0.999 for UV and r2>0.99 for MS) for standard curves were achieved for each isoflavone. The accuracy and repeatability (n=10) were within 15% for these 10 compounds. This is the first method reported that enables the simultaneous quantitation of all 10 isoflavone aglycones in red clover and related species. © 2003 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_US
dc.relation.ispartofJournal of Chromatography Aen_US
dc.subject.meshChromatography, High Pressure Liquid - Methodsen_US
dc.subject.meshHydrolysisen_US
dc.subject.meshIsoflavones - Analysisen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSpectrometry, Mass, Electrospray Ionization - Methodsen_US
dc.subject.meshSpectrophotometry, Ultraviolet - Methodsen_US
dc.subject.meshTrifolium - Chemistryen_US
dc.titleDetermination of isoflavones in red clover and related species by high-performance liquid chromatography combined with ultraviolet and mass spectrometric detectionen_US
dc.typeArticleen_US
dc.identifier.emailWang, M: mfwang@hku.hken_US
dc.identifier.authorityWang, M=rp00800en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.chroma.2003.08.001en_US
dc.identifier.pmid14601839-
dc.identifier.scopuseid_2-s2.0-0141670634en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141670634&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1016en_US
dc.identifier.issue2en_US
dc.identifier.spage195en_US
dc.identifier.epage209en_US
dc.identifier.isiWOS:000185689900006-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridWu, Q=7404602467en_US
dc.identifier.scopusauthoridWang, M=7406691844en_US
dc.identifier.scopusauthoridSimon, JE=7403956069en_US

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