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Article: Production and rapid extraction of lutein and the other lipid-soluble pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditions

TitleProduction and rapid extraction of lutein and the other lipid-soluble pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditions
Authors
Issue Date1999
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's website is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-4133
Citation
Nahrung - Food, 1999, v. 43 n. 2, p. 109-113 How to Cite?
AbstractChlorella protothecoides was successfully cultivated under mixotrophic and heterotrophic conditions. The maximum biomass concentrations of the alga grown at an initial glucose concentration of 40 g/l in the light and in the dark were 22.4 g/l and 17.2 g/l dry cells, respectively. A mechanical method using a new model of dispersing instrument, UltaTurrax T25, was developed for the extraction of the pigments from C. protothecoides cells. Lutein and the other lipid-soluble pigments were separated well by an HPLC system. The contents of lutein, α-carotene, β-carotene, chlorophyll a and chlorophyll b extracted by the mechanical method from the heterotrophic and mixotrophic C. protothecoides cells were 4.43, 0.32, 0.94, 43.76, 9.14 and 6.48, 0.55, 0.66, 56.75, 9.78 mg/g dry cells, respectively. The recoveries of the five pigments through the mechanical extraction were 97.5-99.6%. In contrast, no chlorophylls a and b were obtained through the alkali extraction.
Persistent Identifierhttp://hdl.handle.net/10722/178810
ISSN
2006 Impact Factor: 0.978
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShi, XMen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:49:52Z-
dc.date.available2012-12-19T09:49:52Z-
dc.date.issued1999en_US
dc.identifier.citationNahrung - Food, 1999, v. 43 n. 2, p. 109-113en_US
dc.identifier.issn0027-769Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/178810-
dc.description.abstractChlorella protothecoides was successfully cultivated under mixotrophic and heterotrophic conditions. The maximum biomass concentrations of the alga grown at an initial glucose concentration of 40 g/l in the light and in the dark were 22.4 g/l and 17.2 g/l dry cells, respectively. A mechanical method using a new model of dispersing instrument, UltaTurrax T25, was developed for the extraction of the pigments from C. protothecoides cells. Lutein and the other lipid-soluble pigments were separated well by an HPLC system. The contents of lutein, α-carotene, β-carotene, chlorophyll a and chlorophyll b extracted by the mechanical method from the heterotrophic and mixotrophic C. protothecoides cells were 4.43, 0.32, 0.94, 43.76, 9.14 and 6.48, 0.55, 0.66, 56.75, 9.78 mg/g dry cells, respectively. The recoveries of the five pigments through the mechanical extraction were 97.5-99.6%. In contrast, no chlorophylls a and b were obtained through the alkali extraction.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's website is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-4133-
dc.relation.ispartofNahrung - Fooden_US
dc.titleProduction and rapid extraction of lutein and the other lipid-soluble pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditionsen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1521-3803(19990301)43:2<109::AID-FOOD109>3.0.CO;2-Ken_US
dc.identifier.scopuseid_2-s2.0-0042635675en_US
dc.identifier.hkuros45421-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042635675&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume43en_US
dc.identifier.issue2en_US
dc.identifier.spage109en_US
dc.identifier.epage113en_US
dc.identifier.isiWOS:000079967400008-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridShi, XM=7402953728en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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