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- Publisher Website: 10.1002/(SICI)1521-3803(19990301)43:2<109::AID-FOOD109>3.0.CO;2-K
- Scopus: eid_2-s2.0-0042635675
- WOS: WOS:000079967400008
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Article: Production and rapid extraction of lutein and the other lipid-soluble pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditions
Title | Production and rapid extraction of lutein and the other lipid-soluble pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditions |
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Authors | |
Issue Date | 1999 |
Publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's website is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-4133 |
Citation | Nahrung - Food, 1999, v. 43 n. 2, p. 109-113 How to Cite? |
Abstract | Chlorella protothecoides was successfully cultivated under mixotrophic and heterotrophic conditions. The maximum biomass concentrations of the alga grown at an initial glucose concentration of 40 g/l in the light and in the dark were 22.4 g/l and 17.2 g/l dry cells, respectively. A mechanical method using a new model of dispersing instrument, UltaTurrax T25, was developed for the extraction of the pigments from C. protothecoides cells. Lutein and the other lipid-soluble pigments were separated well by an HPLC system. The contents of lutein, α-carotene, β-carotene, chlorophyll a and chlorophyll b extracted by the mechanical method from the heterotrophic and mixotrophic C. protothecoides cells were 4.43, 0.32, 0.94, 43.76, 9.14 and 6.48, 0.55, 0.66, 56.75, 9.78 mg/g dry cells, respectively. The recoveries of the five pigments through the mechanical extraction were 97.5-99.6%. In contrast, no chlorophylls a and b were obtained through the alkali extraction. |
Persistent Identifier | http://hdl.handle.net/10722/178810 |
ISSN | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Shi, XM | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:49:52Z | - |
dc.date.available | 2012-12-19T09:49:52Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Nahrung - Food, 1999, v. 43 n. 2, p. 109-113 | en_US |
dc.identifier.issn | 0027-769X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178810 | - |
dc.description.abstract | Chlorella protothecoides was successfully cultivated under mixotrophic and heterotrophic conditions. The maximum biomass concentrations of the alga grown at an initial glucose concentration of 40 g/l in the light and in the dark were 22.4 g/l and 17.2 g/l dry cells, respectively. A mechanical method using a new model of dispersing instrument, UltaTurrax T25, was developed for the extraction of the pigments from C. protothecoides cells. Lutein and the other lipid-soluble pigments were separated well by an HPLC system. The contents of lutein, α-carotene, β-carotene, chlorophyll a and chlorophyll b extracted by the mechanical method from the heterotrophic and mixotrophic C. protothecoides cells were 4.43, 0.32, 0.94, 43.76, 9.14 and 6.48, 0.55, 0.66, 56.75, 9.78 mg/g dry cells, respectively. The recoveries of the five pigments through the mechanical extraction were 97.5-99.6%. In contrast, no chlorophylls a and b were obtained through the alkali extraction. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's website is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-4133 | - |
dc.relation.ispartof | Nahrung - Food | en_US |
dc.title | Production and rapid extraction of lutein and the other lipid-soluble pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditions | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/(SICI)1521-3803(19990301)43:2<109::AID-FOOD109>3.0.CO;2-K | en_US |
dc.identifier.scopus | eid_2-s2.0-0042635675 | en_US |
dc.identifier.hkuros | 45421 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0042635675&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 43 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 109 | en_US |
dc.identifier.epage | 113 | en_US |
dc.identifier.isi | WOS:000079967400008 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Shi, XM=7402953728 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0027-769X | - |