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Article: Preparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography

TitlePreparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatography
Authors
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2003, v. 994 n. 1-2, p. 37-43 How to Cite?
AbstractHigh-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent system composed of n-hexane-methanol (2:1, v/v). The upper phase as the mobile phase was pumped into the column at a flow-rate of 2.0 ml min-1 in the tail-to-head elution mode. The fractions purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.2 mg squalene at 96% purity from 150 mg of the crude squalene (0.14% squalene) with 95% recovery. The separation of squalene by HSCCC was completed in 90 min. © 2003 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178804
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, HTen_US
dc.contributor.authorJiang, Yen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:49:50Z-
dc.date.available2012-12-19T09:49:50Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Chromatography A, 2003, v. 994 n. 1-2, p. 37-43en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://hdl.handle.net/10722/178804-
dc.description.abstractHigh-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was obtained from the microalga Thraustochytrium ATCC 26185 by extraction with organic solvents. The crude squalene was further separated using a waterless two-phase solvent system composed of n-hexane-methanol (2:1, v/v). The upper phase as the mobile phase was pumped into the column at a flow-rate of 2.0 ml min-1 in the tail-to-head elution mode. The fractions purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.2 mg squalene at 96% purity from 150 mg of the crude squalene (0.14% squalene) with 95% recovery. The separation of squalene by HSCCC was completed in 90 min. © 2003 Elsevier Science B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_US
dc.relation.ispartofJournal of Chromatography Aen_US
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.-
dc.subject.meshCountercurrent Distribution - Methodsen_US
dc.subject.meshEukaryota - Chemistryen_US
dc.subject.meshSqualene - Isolation & Purificationen_US
dc.titlePreparative separation and purification of squalene from the microalga Thraustochytrium ATCC 26185 by high-speed counter-current chromatographyen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0021-9673(03)00454-0en_US
dc.identifier.pmid12779217-
dc.identifier.scopuseid_2-s2.0-0038667517en_US
dc.identifier.hkuros80793-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038667517&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume994en_US
dc.identifier.issue1-2en_US
dc.identifier.spage37en_US
dc.identifier.epage43en_US
dc.identifier.isiWOS:000182743500003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLu, HT=7404843255en_US
dc.identifier.scopusauthoridJiang, Y=24605346600en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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