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Article: Water self-diffusion behavior in yeast cells studied by pulsed field gradient NMR

TitleWater self-diffusion behavior in yeast cells studied by pulsed field gradient NMR
Authors
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biophyschem
Citation
Biophysical Chemistry, 2003, v. 104 n. 1, p. 121-130 How to Cite?
AbstractThe water self-diffusion behavior in yeast cell water suspension was investigated by pulsed field gradient NMR techniques. Three types of water were detected, which differ according to the self-diffusion coefficients: bulk water, extracellular and intracellular water. Intracellular and extracellular water self-diffusion was restricted; the sizes of restriction regions were approximately 3 and 15-20 μm, respectively. The smallest restriction size was determined as inner cell size. This size and also cell permeability varied with the growth phase of yeast cell. Cell size increased, but permeability decreased with increasing growth time. The values of cell permeabilities P 1 d obtained from time dependence of water self-diffusion coefficient were in good agreement with the permeabilities obtained from the exchange rate constants P 1 eff. The values of P 1 eff were 7×10 -6, 1.2×10 -6 and 1.6×10 -6 m/s, and P 1 d were 6.3×10 -6, 8.4×10 -7, 1.5×10 -6 m/s for yeast cells incubated for 9 h (exponential growth phase), 24 h (end of exponential growth phase), and 48 h (stationary growth phase), respectively. © 2003 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178802
ISSN
2015 Impact Factor: 2.363
2015 SCImago Journal Rankings: 0.929
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSuh, KJen_US
dc.contributor.authorHong, YSen_US
dc.contributor.authorSkirda, VDen_US
dc.contributor.authorVolkov, VIen_US
dc.contributor.authorLee, CYen_US
dc.contributor.authorLee, CHen_US
dc.date.accessioned2012-12-19T09:49:50Z-
dc.date.available2012-12-19T09:49:50Z-
dc.date.issued2003en_US
dc.identifier.citationBiophysical Chemistry, 2003, v. 104 n. 1, p. 121-130en_US
dc.identifier.issn0301-4622en_US
dc.identifier.urihttp://hdl.handle.net/10722/178802-
dc.description.abstractThe water self-diffusion behavior in yeast cell water suspension was investigated by pulsed field gradient NMR techniques. Three types of water were detected, which differ according to the self-diffusion coefficients: bulk water, extracellular and intracellular water. Intracellular and extracellular water self-diffusion was restricted; the sizes of restriction regions were approximately 3 and 15-20 μm, respectively. The smallest restriction size was determined as inner cell size. This size and also cell permeability varied with the growth phase of yeast cell. Cell size increased, but permeability decreased with increasing growth time. The values of cell permeabilities P 1 d obtained from time dependence of water self-diffusion coefficient were in good agreement with the permeabilities obtained from the exchange rate constants P 1 eff. The values of P 1 eff were 7×10 -6, 1.2×10 -6 and 1.6×10 -6 m/s, and P 1 d were 6.3×10 -6, 8.4×10 -7, 1.5×10 -6 m/s for yeast cells incubated for 9 h (exponential growth phase), 24 h (end of exponential growth phase), and 48 h (stationary growth phase), respectively. © 2003 Elsevier Science B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biophyschemen_US
dc.relation.ispartofBiophysical Chemistryen_US
dc.subject.meshBiological Transport, Activeen_US
dc.subject.meshCell Membrane Permeability - Physiologyen_US
dc.subject.meshDiffusionen_US
dc.subject.meshMagnetic Resonance Spectroscopy - Methodsen_US
dc.subject.meshWater - Metabolismen_US
dc.subject.meshYeasts - Growth & Development - Physiologyen_US
dc.titleWater self-diffusion behavior in yeast cells studied by pulsed field gradient NMRen_US
dc.typeArticleen_US
dc.identifier.emailLee, CY: jettylee@hku.hken_US
dc.identifier.authorityLee, CY=rp01511en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0301-4622(02)00361-7en_US
dc.identifier.pmid12834832-
dc.identifier.scopuseid_2-s2.0-0038354507en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038354507&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume104en_US
dc.identifier.issue1en_US
dc.identifier.spage121en_US
dc.identifier.epage130en_US
dc.identifier.isiWOS:000184020500012-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridSuh, KJ=7202644758en_US
dc.identifier.scopusauthoridHong, YS=7403393492en_US
dc.identifier.scopusauthoridSkirda, VD=35580503700en_US
dc.identifier.scopusauthoridVolkov, VI=7402984029en_US
dc.identifier.scopusauthoridLee, CY=13104265200en_US
dc.identifier.scopusauthoridLee, CH=7410151030en_US

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