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Article: Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy

TitleDetermination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy
Authors
Issue Date2003
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 2003, v. 75 n. 10, p. 2440-2444 How to Cite?
AbstractA method has been established for the identification of proanthocyanidins and quantification of individual monoproanthocyanidins using liquid chromatography/electrospray ionization-mass spectrometric detection (LC/ESI-MSD) for raw grape products. The separated monoproanthocyanidins and oligoproanthocyanidins were individually analyzed and identified by their molecular ion peaks using LC/MS. Using HPLC/ESI-MSD, the proanthocyanidin monomers, (+)-catechin (C), (-)-epicatechin (EC), (-)-catechin gallate (CG), and (-)-epicatechin gallate (ECG) in grape products were successfully quantified by LC/MS/MS detection of protonated molecular ions and characteristic fragment ions for each component under the optimized low collision energy level of 20%. For the investigated concentration ranges of C (21.88-11 200 ng/mL), EC (21.10-10 800 ng/mL), CG (36.72-18 800 ng/mL), and ECG (39.84-20 400 ng/mL), good linearities (r 2 > 0.99) for standard curves were obtained. Validation of this method showed an accuracy that was well below 15% and precision (RSD) within 8% for the four compounds. The method proposed here is simple, sensitive, and allows a direct sample preparation procedure. This is the first method that enables the determination of individual monoproanthocyanidins in grape products without any solid-phase extraction.
Persistent Identifierhttp://hdl.handle.net/10722/178797
ISSN
2015 Impact Factor: 5.886
2015 SCImago Journal Rankings: 2.369
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, Qen_US
dc.contributor.authorWang, Men_US
dc.contributor.authorSimon, JEen_US
dc.date.accessioned2012-12-19T09:49:48Z-
dc.date.available2012-12-19T09:49:48Z-
dc.date.issued2003en_US
dc.identifier.citationAnalytical Chemistry, 2003, v. 75 n. 10, p. 2440-2444en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://hdl.handle.net/10722/178797-
dc.description.abstractA method has been established for the identification of proanthocyanidins and quantification of individual monoproanthocyanidins using liquid chromatography/electrospray ionization-mass spectrometric detection (LC/ESI-MSD) for raw grape products. The separated monoproanthocyanidins and oligoproanthocyanidins were individually analyzed and identified by their molecular ion peaks using LC/MS. Using HPLC/ESI-MSD, the proanthocyanidin monomers, (+)-catechin (C), (-)-epicatechin (EC), (-)-catechin gallate (CG), and (-)-epicatechin gallate (ECG) in grape products were successfully quantified by LC/MS/MS detection of protonated molecular ions and characteristic fragment ions for each component under the optimized low collision energy level of 20%. For the investigated concentration ranges of C (21.88-11 200 ng/mL), EC (21.10-10 800 ng/mL), CG (36.72-18 800 ng/mL), and ECG (39.84-20 400 ng/mL), good linearities (r 2 > 0.99) for standard curves were obtained. Validation of this method showed an accuracy that was well below 15% and precision (RSD) within 8% for the four compounds. The method proposed here is simple, sensitive, and allows a direct sample preparation procedure. This is the first method that enables the determination of individual monoproanthocyanidins in grape products without any solid-phase extraction.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_US
dc.relation.ispartofAnalytical Chemistryen_US
dc.subject.meshAnthocyanins - Analysis - Chemistryen_US
dc.subject.meshChromatography, Liquid - Methodsen_US
dc.subject.meshMass Spectrometry - Methodsen_US
dc.subject.meshProanthocyanidinsen_US
dc.subject.meshVitis - Chemistryen_US
dc.titleDetermination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energyen_US
dc.typeArticleen_US
dc.identifier.emailWang, M: mfwang@hku.hken_US
dc.identifier.authorityWang, M=rp00800en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ac0262311en_US
dc.identifier.pmid12918988-
dc.identifier.scopuseid_2-s2.0-0037953069en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037953069&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume75en_US
dc.identifier.issue10en_US
dc.identifier.spage2440en_US
dc.identifier.epage2444en_US
dc.identifier.isiWOS:000182944600033-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWu, Q=7404602467en_US
dc.identifier.scopusauthoridWang, M=7406691844en_US
dc.identifier.scopusauthoridSimon, JE=7403956069en_US

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