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Article: The interplay of collagen IV, tumor necrosis factor-α, gelatinase B (matrix metalloprotease-9), and tissue inhibitor of metalloproteases-1 in the basal lamina regulates sertoli cell-tight junction dynamics in the rat testis

TitleThe interplay of collagen IV, tumor necrosis factor-α, gelatinase B (matrix metalloprotease-9), and tissue inhibitor of metalloproteases-1 in the basal lamina regulates sertoli cell-tight junction dynamics in the rat testis
Authors
Issue Date2003
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2003, v. 144 n. 1, p. 371-387 How to Cite?
AbstractDuring spermatogenesis, preleptotene and leptotene spermatocytes must translocate across the blood-testis barrier formed by inter-Sertoli cell-tight junctions (TJs) from the basal compartment of the seminiferous epithelium adjacent to the basement membrane to the adluminal compartment at stages VIII-IX for further development. Because of the close proximity between extracellular matrix (ECM) that constitutes the basement membrane and the blood-testis barrier, we sought to investigate the role of ECM in Sertoli cell TJ dynamics. When Sertoli cells were cultured in vitro to initiate the assembly of the Sertoli cell TJ-permeability barrier, the presence of an anticollagen IV antibody indeed perturbed the barrier. Because ECM is known to maintain a pool of cytokines and TNFα has been shown to regulate TJ dynamics in other epithelia, we investigated whether TNFα can regulate Sertoli cell TJ function via its effects on collagen α3(IV) and other proteins that maintain the homeostasis of ECM. As expected, recombinant TNFα perturbed the Sertoli cell TJ-barrier assembly in vitro dose dependently. TNFα also inhibited the timely induction of occludin, which is known to associate with the Sertoli cell TJ-barrier assembly. Furthermore, TNFα induced the expression of Sertoli cell collagen α3(IV), gelatinase B (matrix metalloprotease-9, MMP-9) and tissue inhibitor of metalloproteases-1 but not gelatinase A (matrix metalloprotease-2), and promoted the activation of pro-MMP-9. These results thus suggest that the activated MMP-9 induced by TNFα is used to cleave the existing collagen network in the ECM, thereby perturbing the TJ-barrier. This in turn creates a negative feedback that causes TNFα to induce collagen α3(IV) and tissue inhibitor of metalloproteases-1 expression so as to replenish the collagen network in the disrupted TJ-barrier and limit the activity of MMP-9. Taken collectively, these observations strengthen the notion that ECM is involved in the regulation of junction dynamics in addition to its structural role in the testis.
Persistent Identifierhttp://hdl.handle.net/10722/178786
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSiu, MKYen_US
dc.contributor.authorLee, WMen_US
dc.contributor.authorCheng, CYen_US
dc.date.accessioned2012-12-19T09:49:44Z-
dc.date.available2012-12-19T09:49:44Z-
dc.date.issued2003en_US
dc.identifier.citationEndocrinology, 2003, v. 144 n. 1, p. 371-387en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/178786-
dc.description.abstractDuring spermatogenesis, preleptotene and leptotene spermatocytes must translocate across the blood-testis barrier formed by inter-Sertoli cell-tight junctions (TJs) from the basal compartment of the seminiferous epithelium adjacent to the basement membrane to the adluminal compartment at stages VIII-IX for further development. Because of the close proximity between extracellular matrix (ECM) that constitutes the basement membrane and the blood-testis barrier, we sought to investigate the role of ECM in Sertoli cell TJ dynamics. When Sertoli cells were cultured in vitro to initiate the assembly of the Sertoli cell TJ-permeability barrier, the presence of an anticollagen IV antibody indeed perturbed the barrier. Because ECM is known to maintain a pool of cytokines and TNFα has been shown to regulate TJ dynamics in other epithelia, we investigated whether TNFα can regulate Sertoli cell TJ function via its effects on collagen α3(IV) and other proteins that maintain the homeostasis of ECM. As expected, recombinant TNFα perturbed the Sertoli cell TJ-barrier assembly in vitro dose dependently. TNFα also inhibited the timely induction of occludin, which is known to associate with the Sertoli cell TJ-barrier assembly. Furthermore, TNFα induced the expression of Sertoli cell collagen α3(IV), gelatinase B (matrix metalloprotease-9, MMP-9) and tissue inhibitor of metalloproteases-1 but not gelatinase A (matrix metalloprotease-2), and promoted the activation of pro-MMP-9. These results thus suggest that the activated MMP-9 induced by TNFα is used to cleave the existing collagen network in the ECM, thereby perturbing the TJ-barrier. This in turn creates a negative feedback that causes TNFα to induce collagen α3(IV) and tissue inhibitor of metalloproteases-1 expression so as to replenish the collagen network in the disrupted TJ-barrier and limit the activity of MMP-9. Taken collectively, these observations strengthen the notion that ECM is involved in the regulation of junction dynamics in addition to its structural role in the testis.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.rightsEndocrinology. Copyright © The Endocrine Society.-
dc.subject.meshAnimalsen_US
dc.subject.meshCollagen Type Iv - Genetics - Physiologyen_US
dc.subject.meshExtracellular Matrix - Physiologyen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshMatrix Metalloproteinase 2 - Geneticsen_US
dc.subject.meshMatrix Metalloproteinase 9 - Analysis - Genetics - Physiologyen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshRecombinant Proteins - Pharmacologyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSertoli Cells - Chemistry - Ultrastructureen_US
dc.subject.meshSpermatozoa - Chemistryen_US
dc.subject.meshTestis - Chemistry - Ultrastructureen_US
dc.subject.meshTight Junctions - Physiologyen_US
dc.subject.meshTissue Inhibitor Of Metalloproteinase-1 - Genetics - Physiologyen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Genetics - Pharmacology - Physiologyen_US
dc.titleThe interplay of collagen IV, tumor necrosis factor-α, gelatinase B (matrix metalloprotease-9), and tissue inhibitor of metalloproteases-1 in the basal lamina regulates sertoli cell-tight junction dynamics in the rat testisen_US
dc.typeArticleen_US
dc.identifier.emailSiu, MKY: mkysiu@hkucc.hku.hken_US
dc.identifier.emailLee, WM: hrszlwm@hku.hken_US
dc.identifier.authoritySiu, MKY=rp00275en_US
dc.identifier.authorityLee, WM=rp00728en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/en.2002-220786en_US
dc.identifier.pmid12488366-
dc.identifier.scopuseid_2-s2.0-0037230154en_US
dc.identifier.hkuros114329-
dc.identifier.hkuros81138-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037230154&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume144en_US
dc.identifier.issue1en_US
dc.identifier.spage371en_US
dc.identifier.epage387en_US
dc.identifier.isiWOS:000180176100046-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSiu, MKY=24924018400en_US
dc.identifier.scopusauthoridLee, WM=24799156600en_US
dc.identifier.scopusauthoridCheng, CY=7404797787en_US

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