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Article: Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after saponification

TitleIsolation and purification of lutein from the microalga Chlorella vulgaris by extraction after saponification
Authors
KeywordsChlorella Vulgaris
Extraction
Lutein
Purification
Saponification
Issue Date2002
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 2002, v. 50 n. 5, p. 1070-1072 How to Cite?
AbstractA simple and efficient method for the isolation and purification of lutein from the microalga Chlorella vulgaris was developed. Crude lutein was obtained by extraction with dichloromethane from the microalga after saponification. Partition values of lutein in the two-phase system of ethanol-water-dichloromethane at different ratios were measured by HPLC so as to assist the determination of an appropriate condition for washing water-soluble impurities in the crude lutein. Partition values of lutein in another two-phase system of ethanol-water-hexane at different ratios were also measured by HPLC for determining the condition for removing fat-soluble impurities. The water-soluble impurities in the crude lutein were removed by washing with 30% aqueous ethanol, and the fat-soluble impurities were removed by extraction with hexane. The final purity of lutein obtained was 90-98%, and the yield was 85-91%.
Persistent Identifierhttp://hdl.handle.net/10722/178784
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_US
dc.contributor.authorJiang, Yen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:49:44Z-
dc.date.available2012-12-19T09:49:44Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2002, v. 50 n. 5, p. 1070-1072en_US
dc.identifier.issn0021-8561en_US
dc.identifier.urihttp://hdl.handle.net/10722/178784-
dc.description.abstractA simple and efficient method for the isolation and purification of lutein from the microalga Chlorella vulgaris was developed. Crude lutein was obtained by extraction with dichloromethane from the microalga after saponification. Partition values of lutein in the two-phase system of ethanol-water-dichloromethane at different ratios were measured by HPLC so as to assist the determination of an appropriate condition for washing water-soluble impurities in the crude lutein. Partition values of lutein in another two-phase system of ethanol-water-hexane at different ratios were also measured by HPLC for determining the condition for removing fat-soluble impurities. The water-soluble impurities in the crude lutein were removed by washing with 30% aqueous ethanol, and the fat-soluble impurities were removed by extraction with hexane. The final purity of lutein obtained was 90-98%, and the yield was 85-91%.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_US
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_US
dc.subjectChlorella Vulgarisen_US
dc.subjectExtractionen_US
dc.subjectLuteinen_US
dc.subjectPurificationen_US
dc.subjectSaponificationen_US
dc.titleIsolation and purification of lutein from the microalga Chlorella vulgaris by extraction after saponificationen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/jf010220ben_US
dc.identifier.pmid11853482-
dc.identifier.scopuseid_2-s2.0-0037180967en_US
dc.identifier.hkuros72002-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037180967&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume50en_US
dc.identifier.issue5en_US
dc.identifier.spage1070en_US
dc.identifier.epage1072en_US
dc.identifier.isiWOS:000173995400020-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLi, HB=39461695700en_US
dc.identifier.scopusauthoridJiang, Y=24605346600en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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