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- Publisher Website: 10.1021/jf010220b
- Scopus: eid_2-s2.0-0037180967
- PMID: 11853482
- WOS: WOS:000173995400020
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Article: Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after saponification
Title | Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after saponification |
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Authors | |
Keywords | Chlorella Vulgaris Extraction Lutein Purification Saponification |
Issue Date | 2002 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau |
Citation | Journal Of Agricultural And Food Chemistry, 2002, v. 50 n. 5, p. 1070-1072 How to Cite? |
Abstract | A simple and efficient method for the isolation and purification of lutein from the microalga Chlorella vulgaris was developed. Crude lutein was obtained by extraction with dichloromethane from the microalga after saponification. Partition values of lutein in the two-phase system of ethanol-water-dichloromethane at different ratios were measured by HPLC so as to assist the determination of an appropriate condition for washing water-soluble impurities in the crude lutein. Partition values of lutein in another two-phase system of ethanol-water-hexane at different ratios were also measured by HPLC for determining the condition for removing fat-soluble impurities. The water-soluble impurities in the crude lutein were removed by washing with 30% aqueous ethanol, and the fat-soluble impurities were removed by extraction with hexane. The final purity of lutein obtained was 90-98%, and the yield was 85-91%. |
Persistent Identifier | http://hdl.handle.net/10722/178784 |
ISSN | 2021 Impact Factor: 5.895 2020 SCImago Journal Rankings: 1.203 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Jiang, Y | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:49:44Z | - |
dc.date.available | 2012-12-19T09:49:44Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Journal Of Agricultural And Food Chemistry, 2002, v. 50 n. 5, p. 1070-1072 | en_US |
dc.identifier.issn | 0021-8561 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178784 | - |
dc.description.abstract | A simple and efficient method for the isolation and purification of lutein from the microalga Chlorella vulgaris was developed. Crude lutein was obtained by extraction with dichloromethane from the microalga after saponification. Partition values of lutein in the two-phase system of ethanol-water-dichloromethane at different ratios were measured by HPLC so as to assist the determination of an appropriate condition for washing water-soluble impurities in the crude lutein. Partition values of lutein in another two-phase system of ethanol-water-hexane at different ratios were also measured by HPLC for determining the condition for removing fat-soluble impurities. The water-soluble impurities in the crude lutein were removed by washing with 30% aqueous ethanol, and the fat-soluble impurities were removed by extraction with hexane. The final purity of lutein obtained was 90-98%, and the yield was 85-91%. | en_US |
dc.language | eng | en_US |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau | en_US |
dc.relation.ispartof | Journal of Agricultural and Food Chemistry | en_US |
dc.subject | Chlorella Vulgaris | en_US |
dc.subject | Extraction | en_US |
dc.subject | Lutein | en_US |
dc.subject | Purification | en_US |
dc.subject | Saponification | en_US |
dc.title | Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after saponification | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1021/jf010220b | en_US |
dc.identifier.pmid | 11853482 | - |
dc.identifier.scopus | eid_2-s2.0-0037180967 | en_US |
dc.identifier.hkuros | 72002 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037180967&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 50 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 1070 | en_US |
dc.identifier.epage | 1072 | en_US |
dc.identifier.isi | WOS:000173995400020 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Li, HB=39461695700 | en_US |
dc.identifier.scopusauthorid | Jiang, Y=24605346600 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0021-8561 | - |