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Article: Perfusion culture of the diatom Nitzschia laevis for ultra-high yield of eicosapentaenoic acid

TitlePerfusion culture of the diatom Nitzschia laevis for ultra-high yield of eicosapentaenoic acid
Authors
KeywordsDiatom
Eicosapentaenoic Acid
High Yield
Nitzschia Laevis
Perfusion
Issue Date2002
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 2002, v. 38 n. 4, p. 523-529 How to Cite?
AbstractIn the development of high yield of eicosapentaenoic acid (EPA) by the diatom Nitzschia laevis, substrate limitation and toxic metabolite inhibition were the two principal obstacles. In the present study, a perfusion culture was developed to eliminate substrate (glucose) limitation and by-product inhibition simultaneously. Using an exponential feed strategy and manipulating glucose supply rate and by-product removal rate, the optimal glucose concentration in the feed medium was determined to be 50 g l-1. With this feed glucose concentration (S0 = 50 g l-1), the perfusion culture resulted to a cell dry weight concentration of 40 g l-1. Such a high cell density led to an EPA yield of 1112 mg l-1, which is the highest ever reported in microalgal cultures. In addition, the perfusion culture (at S0 = 50 g l-1) greatly reduced the amount of wastewater (broth) during cultures. Considering the simple set-up and operation, the proposed perfusion culture is potentially useful for large scale EPA production by N. laevis. © 2002 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178779
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWen, ZYen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:49:42Z-
dc.date.available2012-12-19T09:49:42Z-
dc.date.issued2002en_US
dc.identifier.citationProcess Biochemistry, 2002, v. 38 n. 4, p. 523-529en_US
dc.identifier.issn1359-5113en_US
dc.identifier.urihttp://hdl.handle.net/10722/178779-
dc.description.abstractIn the development of high yield of eicosapentaenoic acid (EPA) by the diatom Nitzschia laevis, substrate limitation and toxic metabolite inhibition were the two principal obstacles. In the present study, a perfusion culture was developed to eliminate substrate (glucose) limitation and by-product inhibition simultaneously. Using an exponential feed strategy and manipulating glucose supply rate and by-product removal rate, the optimal glucose concentration in the feed medium was determined to be 50 g l-1. With this feed glucose concentration (S0 = 50 g l-1), the perfusion culture resulted to a cell dry weight concentration of 40 g l-1. Such a high cell density led to an EPA yield of 1112 mg l-1, which is the highest ever reported in microalgal cultures. In addition, the perfusion culture (at S0 = 50 g l-1) greatly reduced the amount of wastewater (broth) during cultures. Considering the simple set-up and operation, the proposed perfusion culture is potentially useful for large scale EPA production by N. laevis. © 2002 Elsevier Science Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_US
dc.relation.ispartofProcess Biochemistryen_US
dc.rightsProcess Biochemistry. Copyright © Elsevier Ltd.-
dc.subjectDiatomen_US
dc.subjectEicosapentaenoic Aciden_US
dc.subjectHigh Yielden_US
dc.subjectNitzschia Laevisen_US
dc.subjectPerfusionen_US
dc.titlePerfusion culture of the diatom Nitzschia laevis for ultra-high yield of eicosapentaenoic aciden_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0032-9592(02)00174-7en_US
dc.identifier.scopuseid_2-s2.0-0037010767en_US
dc.identifier.hkuros80809-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037010767&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume38en_US
dc.identifier.issue4en_US
dc.identifier.spage523en_US
dc.identifier.epage529en_US
dc.identifier.isiWOS:000179438000007-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWen, ZY=14012373200en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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