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Article: Effects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitro

TitleEffects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitro
Authors
Issue Date2002
Citation
Acta Biochimica Et Biophysica Sinica, 2002, v. 34 n. 6, p. 790-795 How to Cite?
AbstractIn order to study signal transduction in PACAP-stimulated GH release, effects of ovine PACAPs (oPACAPs) on cAMP synthesis and intracellular free Ca 2+ ([Ca 2+ ] i) levels in primarily cultured common carp (Cyprinus carpio) pituitary cells were investigated using radioimmunoassay (RIA) for cAMP and [Ca 2+ ] i Indo1/AM loading techniques. The main results obtained could be briefly summarized as follows: parallel with their GH-releasing effects, oPACAP-38 (0.01 nmol/L to 1 μmol/L) and oPACAP-27 (0.01 nmol/L to 1 μmol/L) were also effective in increasing cAMP release, cellular cAMP content, and total cAMP production in a dose-dependent manner in common carp pituitary cells. In addition to their cAMP synthesis, 0.5 μmol/L oPACAP-38 and 0.5 μmol/L oPACAP-27 were also effective in elevating [Ca 2+ ] i levels in common carp pituitary cells preloaded with the Ca 2+ -sensitive dye Indo-1; the stimulatory actions were dependent on the presence of extracellular Ca 2+ ([Ca 2+ ] e), as removal of [Ca 2+ ] e by adding the Ca 2+ chelator 4 mmol/L EGTA blocked the rise of [Ca 2+ ] i induced by oPACAP-38 and oPACAP-27, respectively. Besides, the rise in [Ca 2+ ] i induced by 0.5 μmol/L oPACAP-38 was blocked by treatment with 10 μmol/L nifedipine, an inhibitor of "L-type" voltage-sensitive Ca 2+ channels (VSCC); but 0.5 μmol/L oPACAP-38 was not effective in elevating [Ca 2+ ] i levels after the cells treated with Ca 2+ -sensitive dye Indo-1 were challenged with 10 μmol/L nifedipine. These results, taken as a whole, strongly suggest the involvement of the cAMP- and Ca 2+ -dependent pathways in PACAP-stimulated GH release in common carp.
Persistent Identifierhttp://hdl.handle.net/10722/178778
ISSN
2003 Impact Factor: 0.524
References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Den_US
dc.contributor.authorChu, MSen_US
dc.contributor.authorLee, KYen_US
dc.contributor.authorWong, AOen_US
dc.contributor.authorLin, HRen_US
dc.date.accessioned2012-12-19T09:49:42Z-
dc.date.available2012-12-19T09:49:42Z-
dc.date.issued2002en_US
dc.identifier.citationActa Biochimica Et Biophysica Sinica, 2002, v. 34 n. 6, p. 790-795en_US
dc.identifier.issn0582-9879en_US
dc.identifier.urihttp://hdl.handle.net/10722/178778-
dc.description.abstractIn order to study signal transduction in PACAP-stimulated GH release, effects of ovine PACAPs (oPACAPs) on cAMP synthesis and intracellular free Ca 2+ ([Ca 2+ ] i) levels in primarily cultured common carp (Cyprinus carpio) pituitary cells were investigated using radioimmunoassay (RIA) for cAMP and [Ca 2+ ] i Indo1/AM loading techniques. The main results obtained could be briefly summarized as follows: parallel with their GH-releasing effects, oPACAP-38 (0.01 nmol/L to 1 μmol/L) and oPACAP-27 (0.01 nmol/L to 1 μmol/L) were also effective in increasing cAMP release, cellular cAMP content, and total cAMP production in a dose-dependent manner in common carp pituitary cells. In addition to their cAMP synthesis, 0.5 μmol/L oPACAP-38 and 0.5 μmol/L oPACAP-27 were also effective in elevating [Ca 2+ ] i levels in common carp pituitary cells preloaded with the Ca 2+ -sensitive dye Indo-1; the stimulatory actions were dependent on the presence of extracellular Ca 2+ ([Ca 2+ ] e), as removal of [Ca 2+ ] e by adding the Ca 2+ chelator 4 mmol/L EGTA blocked the rise of [Ca 2+ ] i induced by oPACAP-38 and oPACAP-27, respectively. Besides, the rise in [Ca 2+ ] i induced by 0.5 μmol/L oPACAP-38 was blocked by treatment with 10 μmol/L nifedipine, an inhibitor of "L-type" voltage-sensitive Ca 2+ channels (VSCC); but 0.5 μmol/L oPACAP-38 was not effective in elevating [Ca 2+ ] i levels after the cells treated with Ca 2+ -sensitive dye Indo-1 were challenged with 10 μmol/L nifedipine. These results, taken as a whole, strongly suggest the involvement of the cAMP- and Ca 2+ -dependent pathways in PACAP-stimulated GH release in common carp.en_US
dc.languageengen_US
dc.relation.ispartofActa Biochimica et Biophysica Sinicaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCalcium Channel Blockers - Pharmacologyen_US
dc.subject.meshCarpsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshNeuropeptides - Pharmacologyen_US
dc.subject.meshNifedipine - Pharmacologyen_US
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptideen_US
dc.subject.meshPituitary Gland - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshSheepen_US
dc.subject.meshTime Factorsen_US
dc.titleEffects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitroen_US
dc.typeArticleen_US
dc.identifier.emailWong, AO: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AO=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12417926-
dc.identifier.scopuseid_2-s2.0-0036944502en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036944502&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume34en_US
dc.identifier.issue6en_US
dc.identifier.spage790en_US
dc.identifier.epage795en_US
dc.identifier.scopusauthoridXiao, D=45561966600en_US
dc.identifier.scopusauthoridChu, MS=7402457255en_US
dc.identifier.scopusauthoridLee, KY=26660356800en_US
dc.identifier.scopusauthoridWong, AO=7403147570en_US
dc.identifier.scopusauthoridLin, HR=7405571313en_US

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