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Article: Recent progress on immobilization of enzymes on molecular sieves for reactions in organic solvents

TitleRecent progress on immobilization of enzymes on molecular sieves for reactions in organic solvents
Authors
KeywordsEnzyme
Immobilization
Matrix
Mcm-41
Molecular Sieve
Zeolite
Issue Date2002
Citation
Applied Biochemistry And Biotechnology - Part A Enzyme Engineering And Biotechnology, 2002, v. 101 n. 2, p. 113-129 How to Cite?
AbstractEnzymes exhibit high selectivity and reactivity under normal conditions but are sensitive to denaturation or inactivation by pH and temperature extremes, organic solvents, and detergents. To extend the use of these biocatalysts for practical applications, the technology of immobilization of enzymes on suitable supports was developed. Recently, these immobilized biomolecules have been widely used and a variety of immobilization supports have been studied. The majority of these supports cover diverse kinds of materials such as natural or synthetic polyhydroxylic matrixes, porous inorganic carriers, and all kinds of functional polymers. Microporous molecular sieve, zeolite, has attracted extensive interest in research because of its distinctive physical properties and geochemistry. Recently, with the discovery of a new family of mesoporous molecular sieves, MCM-41, this series of materials shows great potential for various applications. Molecular sieves involve such a series of materials that can discriminate between molecules, particularly on the basis of size. As support materials, they offer interesting properties, such as high surface areas, hydrophobic or hydrophilic behavior, and electrostatic interaction, as well as mechanical and chemical resistance, making them attractive for enzyme immobilization. In this article, different types of molecular sieves used in different immobilization methods including physical adsorption on zeolite, entrapment in mesoporous and macroporous MCM series, as well as chemically covalent binding to functionalized molecular sieves are reviewed. Key factors affecting the application of this biotechnology are discussed systematically, and immobilization mechanisms combined with newly developed techniques to elucidate the interactions between matrixes and enzyme molecules are also introduced.
Persistent Identifierhttp://hdl.handle.net/10722/178770
ISSN
2015 Impact Factor: 1.606
2015 SCImago Journal Rankings: 0.575
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYan, AXen_US
dc.contributor.authorLi, XWen_US
dc.contributor.authorYe, YHen_US
dc.date.accessioned2012-12-19T09:49:39Z-
dc.date.available2012-12-19T09:49:39Z-
dc.date.issued2002en_US
dc.identifier.citationApplied Biochemistry And Biotechnology - Part A Enzyme Engineering And Biotechnology, 2002, v. 101 n. 2, p. 113-129en_US
dc.identifier.issn0273-2289en_US
dc.identifier.urihttp://hdl.handle.net/10722/178770-
dc.description.abstractEnzymes exhibit high selectivity and reactivity under normal conditions but are sensitive to denaturation or inactivation by pH and temperature extremes, organic solvents, and detergents. To extend the use of these biocatalysts for practical applications, the technology of immobilization of enzymes on suitable supports was developed. Recently, these immobilized biomolecules have been widely used and a variety of immobilization supports have been studied. The majority of these supports cover diverse kinds of materials such as natural or synthetic polyhydroxylic matrixes, porous inorganic carriers, and all kinds of functional polymers. Microporous molecular sieve, zeolite, has attracted extensive interest in research because of its distinctive physical properties and geochemistry. Recently, with the discovery of a new family of mesoporous molecular sieves, MCM-41, this series of materials shows great potential for various applications. Molecular sieves involve such a series of materials that can discriminate between molecules, particularly on the basis of size. As support materials, they offer interesting properties, such as high surface areas, hydrophobic or hydrophilic behavior, and electrostatic interaction, as well as mechanical and chemical resistance, making them attractive for enzyme immobilization. In this article, different types of molecular sieves used in different immobilization methods including physical adsorption on zeolite, entrapment in mesoporous and macroporous MCM series, as well as chemically covalent binding to functionalized molecular sieves are reviewed. Key factors affecting the application of this biotechnology are discussed systematically, and immobilization mechanisms combined with newly developed techniques to elucidate the interactions between matrixes and enzyme molecules are also introduced.en_US
dc.languageengen_US
dc.relation.ispartofApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnologyen_US
dc.subjectEnzymeen_US
dc.subjectImmobilizationen_US
dc.subjectMatrixen_US
dc.subjectMcm-41en_US
dc.subjectMolecular Sieveen_US
dc.subjectZeoliteen_US
dc.titleRecent progress on immobilization of enzymes on molecular sieves for reactions in organic solventsen_US
dc.typeArticleen_US
dc.identifier.emailYan, AX: ayan8@hku.hken_US
dc.identifier.authorityYan, AX=rp00823en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1385/ABAB:101:2:113en_US
dc.identifier.pmid12049202-
dc.identifier.scopuseid_2-s2.0-0036562137en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036562137&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume101en_US
dc.identifier.issue2en_US
dc.identifier.spage113en_US
dc.identifier.epage129en_US
dc.identifier.isiWOS:000175662900002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYan, AX=8621667000en_US
dc.identifier.scopusauthoridLi, XW=12773862200en_US
dc.identifier.scopusauthoridYe, YH=7401627480en_US

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