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Article: Effect of mixing time on taxoid production using suspension cultures of Taxus chinensis in a centrifugal impeller bioreactor

TitleEffect of mixing time on taxoid production using suspension cultures of Taxus chinensis in a centrifugal impeller bioreactor
Authors
KeywordsCentrifugal Impeller Bioreactor
Mixing Time
Oxygen Transfer
Plant Cell Culture
Taxoid Production
Taxus Chinensis
Issue Date2002
PublisherSociety for Biotechnology, Japan. The Journal's web site is located at http://www.jstage.jst.go.jp/browse/jbb/_vols/-char/en
Citation
Journal Of Bioscience And Bioengineering, 2002, v. 94 n. 3, p. 244-250 How to Cite?
AbstractThe effects of fluid mixing on the cell growth and secondary metabolite production of plant cells were investigated in a low-shear centrifugal impeller bioreactor (CIB) system. Suspension cultures of Taxus chinensis cells producing taxuyunnanine C (Tc), a physiologically active secondary metabolite, were used as a model system for this investigation. The mixing time (t m) and volumetric oxygen transfer coefficient (k La) in the bioreactor were characterized at various cell densities and operating conditions. A constant t m of 5 s or 10 s was maintained during cultivation by adjusting the impeller agitation speed with no detrimental effect on the cultured cells. A higher cell density, Tc content and total Tc production were obtained under the shorter mixing time of 5 s. The favorable effect of more rapid mixing on Tc production was also confirmed when the Tc accumulation was significantly increased through culture elicitation using 100 μM methyl jasmonate (MJA). The lower Tc production at the longer t m of 10 s was mainly attributed to oxygen transfer limitation in the dead zones and larger cell aggregates resulting from poor mixing.
Persistent Identifierhttp://hdl.handle.net/10722/178769
ISSN
2015 Impact Factor: 1.964
2015 SCImago Journal Rankings: 0.709
References

 

DC FieldValueLanguage
dc.contributor.authorZhong, JJen_US
dc.contributor.authorPan, ZWen_US
dc.contributor.authorWang, ZYen_US
dc.contributor.authorWu, Jen_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorTakagi, Men_US
dc.contributor.authorYoshida, Ten_US
dc.date.accessioned2012-12-19T09:49:38Z-
dc.date.available2012-12-19T09:49:38Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Bioscience And Bioengineering, 2002, v. 94 n. 3, p. 244-250en_US
dc.identifier.issn1389-1723en_US
dc.identifier.urihttp://hdl.handle.net/10722/178769-
dc.description.abstractThe effects of fluid mixing on the cell growth and secondary metabolite production of plant cells were investigated in a low-shear centrifugal impeller bioreactor (CIB) system. Suspension cultures of Taxus chinensis cells producing taxuyunnanine C (Tc), a physiologically active secondary metabolite, were used as a model system for this investigation. The mixing time (t m) and volumetric oxygen transfer coefficient (k La) in the bioreactor were characterized at various cell densities and operating conditions. A constant t m of 5 s or 10 s was maintained during cultivation by adjusting the impeller agitation speed with no detrimental effect on the cultured cells. A higher cell density, Tc content and total Tc production were obtained under the shorter mixing time of 5 s. The favorable effect of more rapid mixing on Tc production was also confirmed when the Tc accumulation was significantly increased through culture elicitation using 100 μM methyl jasmonate (MJA). The lower Tc production at the longer t m of 10 s was mainly attributed to oxygen transfer limitation in the dead zones and larger cell aggregates resulting from poor mixing.en_US
dc.languageengen_US
dc.publisherSociety for Biotechnology, Japan. The Journal's web site is located at http://www.jstage.jst.go.jp/browse/jbb/_vols/-char/enen_US
dc.relation.ispartofJournal of Bioscience and Bioengineeringen_US
dc.subjectCentrifugal Impeller Bioreactoren_US
dc.subjectMixing Timeen_US
dc.subjectOxygen Transferen_US
dc.subjectPlant Cell Cultureen_US
dc.subjectTaxoid Productionen_US
dc.subjectTaxus Chinensisen_US
dc.titleEffect of mixing time on taxoid production using suspension cultures of Taxus chinensis in a centrifugal impeller bioreactoren_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1263/jbb.94.244en_US
dc.identifier.scopuseid_2-s2.0-0036399916en_US
dc.identifier.hkuros80812-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036399916&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume94en_US
dc.identifier.issue3en_US
dc.identifier.spage244en_US
dc.identifier.epage250en_US
dc.publisher.placeJapanen_US
dc.identifier.scopusauthoridZhong, JJ=7201488646en_US
dc.identifier.scopusauthoridPan, ZW=55467975300en_US
dc.identifier.scopusauthoridWang, ZY=9634425300en_US
dc.identifier.scopusauthoridWu, J=8846838700en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridTakagi, M=7402556966en_US
dc.identifier.scopusauthoridYoshida, T=7501314737en_US

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