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Article: Leakage of cellular materials from Saccharomyces cerevisiae by ohmic heating

TitleLeakage of cellular materials from Saccharomyces cerevisiae by ohmic heating
Authors
KeywordsCell Wall
Electroporation
Ohmic Heating
Permeability
Saccharomyces Cerevisiae
Issue Date2002
PublisherHan'gug Mi'saengmul Saengmyeong Gong Haghoe. The Journal's web site is located at http://www.jmb.or.kr/
Citation
Journal Of Microbiology And Biotechnology, 2002, v. 12 n. 2, p. 183-188 How to Cite?
AbstractThe ohmic heating of foods for sterilization provides a shorter come-up time compared to conventional thermal processes. The electric fields as well as the heat generated by ohmic heating facilitate germicidal effects. In the present study, the effect of ohmic heating on the structure and permeability of the cell membrane of yeast cells, Saccharomyces cerevisae, isolated from Takju (a traditional Korean rice-beer), was investigated. The ohmic heating was found to translocate intracellular protein materials out of the cell wall, and the amount of exuded protein increased significantly as the electric field increased from 10 to 20 V/cm. As higher frequencies were applied, more materials were exuded. Compared to conventional heating, more amounts of proteins and nucleic acids were exuded when these cells were treated with ohmic heating. The molecular weights of the major exuded proteins ranged from 14 kDa to 18 kDa, as analyzed by Tricine-SDS PAGE. A TEM study also confirmed the leakage of cellular materials, thus indicating irreversible damage to the cell wall by ohmic heating. It was, therefore, concluded that the electric fields generated by ohmic heating induced electroporation, causing irreversible damage to the yeast cell wall and promoting the translocation of intracellular materials.
Persistent Identifierhttp://hdl.handle.net/10722/178761
ISSN
2015 Impact Factor: 1.685
2015 SCImago Journal Rankings: 0.548
References

 

DC FieldValueLanguage
dc.contributor.authorYoon, SWen_US
dc.contributor.authorLee, CYJen_US
dc.contributor.authorKim, KMen_US
dc.contributor.authorLee, CHen_US
dc.date.accessioned2012-12-19T09:49:36Z-
dc.date.available2012-12-19T09:49:36Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Microbiology And Biotechnology, 2002, v. 12 n. 2, p. 183-188en_US
dc.identifier.issn1017-7825en_US
dc.identifier.urihttp://hdl.handle.net/10722/178761-
dc.description.abstractThe ohmic heating of foods for sterilization provides a shorter come-up time compared to conventional thermal processes. The electric fields as well as the heat generated by ohmic heating facilitate germicidal effects. In the present study, the effect of ohmic heating on the structure and permeability of the cell membrane of yeast cells, Saccharomyces cerevisae, isolated from Takju (a traditional Korean rice-beer), was investigated. The ohmic heating was found to translocate intracellular protein materials out of the cell wall, and the amount of exuded protein increased significantly as the electric field increased from 10 to 20 V/cm. As higher frequencies were applied, more materials were exuded. Compared to conventional heating, more amounts of proteins and nucleic acids were exuded when these cells were treated with ohmic heating. The molecular weights of the major exuded proteins ranged from 14 kDa to 18 kDa, as analyzed by Tricine-SDS PAGE. A TEM study also confirmed the leakage of cellular materials, thus indicating irreversible damage to the cell wall by ohmic heating. It was, therefore, concluded that the electric fields generated by ohmic heating induced electroporation, causing irreversible damage to the yeast cell wall and promoting the translocation of intracellular materials.en_US
dc.languageengen_US
dc.publisherHan'gug Mi'saengmul Saengmyeong Gong Haghoe. The Journal's web site is located at http://www.jmb.or.kr/en_US
dc.relation.ispartofJournal of Microbiology and Biotechnologyen_US
dc.subjectCell Wallen_US
dc.subjectElectroporationen_US
dc.subjectOhmic Heatingen_US
dc.subjectPermeabilityen_US
dc.subjectSaccharomyces Cerevisiaeen_US
dc.titleLeakage of cellular materials from Saccharomyces cerevisiae by ohmic heatingen_US
dc.typeArticleen_US
dc.identifier.emailLee, CYJ: jettylee@hku.hken_US
dc.identifier.authorityLee, CYJ=rp01511en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0036098172en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036098172&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume12en_US
dc.identifier.issue2en_US
dc.identifier.spage183en_US
dc.identifier.epage188en_US
dc.publisher.placeKorea, Republic ofen_US
dc.identifier.scopusauthoridYoon, SW=13407062300en_US
dc.identifier.scopusauthoridLee, CYJ=13104265200en_US
dc.identifier.scopusauthoridKim, KM=7409318084en_US
dc.identifier.scopusauthoridLee, CH=7410151030en_US

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