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Article: Determination of vitamin B12 in multivitamin tablets and fermentation medium by high-performance liquid chromatography with fluorescence detection

TitleDetermination of vitamin B12 in multivitamin tablets and fermentation medium by high-performance liquid chromatography with fluorescence detection
Authors
Issue Date2000
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2000, v. 891 n. 2, p. 243-247 How to Cite?
AbstractA novel method for the determination of vitamin B12 by high- performance liquid chromatography with fluorescence detection is reported. The method was simple and highly sensitive with good precision. Vitamin B12 was analyzed by HPLC on a μBondapak C18 column (300 x 3.9 mm, 10 μm) with methanol-water (30:70) as mobile phase and fluorescence detection at 305 nm (with excitation at 275 nm). The calibration graph was linear from 1.000 to 100.0 ng ml-1 for vitamin B12 with a correlation coefficient of 0.998 (n = 6). The detection limit was 0.1 ng ml-1. The method was successfully applied to the determination of vitamin B12 in vitamin B12 tablets, multivitamin tablets and fermentation medium. The recovery was from 94 to 102% and the relative standard deviation was in the range of 1.8 to 4.1%. (C) 2000 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/178722
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorJiang, Yen_US
dc.date.accessioned2012-12-19T09:49:20Z-
dc.date.available2012-12-19T09:49:20Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Chromatography A, 2000, v. 891 n. 2, p. 243-247en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://hdl.handle.net/10722/178722-
dc.description.abstractA novel method for the determination of vitamin B12 by high- performance liquid chromatography with fluorescence detection is reported. The method was simple and highly sensitive with good precision. Vitamin B12 was analyzed by HPLC on a μBondapak C18 column (300 x 3.9 mm, 10 μm) with methanol-water (30:70) as mobile phase and fluorescence detection at 305 nm (with excitation at 275 nm). The calibration graph was linear from 1.000 to 100.0 ng ml-1 for vitamin B12 with a correlation coefficient of 0.998 (n = 6). The detection limit was 0.1 ng ml-1. The method was successfully applied to the determination of vitamin B12 in vitamin B12 tablets, multivitamin tablets and fermentation medium. The recovery was from 94 to 102% and the relative standard deviation was in the range of 1.8 to 4.1%. (C) 2000 Elsevier Science B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_US
dc.relation.ispartofJournal of Chromatography Aen_US
dc.subject.meshChromatography, High Pressure Liquid - Methodsen_US
dc.subject.meshCulture Media - Chemistryen_US
dc.subject.meshFermentationen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.subject.meshTablets - Chemistryen_US
dc.subject.meshVitamin B 12 - Analysisen_US
dc.subject.meshVitamins - Chemistryen_US
dc.titleDetermination of vitamin B12 in multivitamin tablets and fermentation medium by high-performance liquid chromatography with fluorescence detectionen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0021-9673(00)00724-Xen_US
dc.identifier.pmid11043784-
dc.identifier.scopuseid_2-s2.0-0034622676en_US
dc.identifier.hkuros61526-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034622676&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume891en_US
dc.identifier.issue2en_US
dc.identifier.spage243en_US
dc.identifier.epage247en_US
dc.identifier.isiWOS:000089286000004-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLi, HB=36066773900en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridJiang, Y=24605346600en_US

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